Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage

Berry, Luke J.; Sheil, Barbara; Garratt, Luke W.; Sly, Peter D.

doi:10.1016/j.jcf.2010.06.002

Cited by 12 publications

(6 citation statements)

References 18 publications

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“…Differences in storing times can have effects on mediator concentrations. For one of the principle parameters, IL-8, this question has been assessed previously by Berry et al finding that storage at temperatures as applied to our samples did not influence IL-8 concentrations for up to 6 years [19]. Additionally, for most mediators assessed within our trial population the longer stored samples resulted significantly higher and/or significantly more often positive for the mediators than samples from our healthy controls.…”

Section: Limitationssupporting

confidence: 54%

“…However, in our cohort concentration differences between CF patients and controls did not reach statistical significance because of relatively high variance of values. Thereby, we exclude storage effects on IL-8 levels, which were proven stable in BAL-fluid for up to 6 years at − 80°C [19], as stored in our CF cohort. IL-8 concentrations might increase with age of subjects, as reported for induced sputum for both CF patients and non-CF controls (r = 0.41; p b 0.05) [31].…”

Section: Il-8mentioning

confidence: 99%

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Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Beiersdorf

Schien

Hentschel

et al. 2013

Journal of Cystic Fibrosis

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Section: Limitationssupporting

confidence: 54%

Section: Il-8mentioning

confidence: 99%

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Beiersdorf

Schien

Hentschel

et al. 2013

Journal of Cystic Fibrosis

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“…Following induction of general anaesthesia, a flexible bronchoscopy through a laryngeal mask airway was performed. BAL was collected in the right middle lobe and lingula (or worst affected lobe on chest CT scans) and sent for microbiological processing to test for the presence of bacteria, fungi and viruses and for analysis of markers of inflammation (20)(21)(22).…”

Section: Bronchoscopy Bal Collection and Processingmentioning

confidence: 99%

Changing Prevalence of Lower Airway Infections in Young Children with Cystic Fibrosis

Breuer

Schultz

Turkovic

et al. 2019

Am J Respir Crit Care Med

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Rationale: Historical studies suggest that airway infection in cystic fibrosis (CF) initiates with Staphylococcus aureus and Haemophilus influenzae with later emergence of Pseudomonas aeruginosa. Aspergillus species are regarded as relatively infrequent, late occurring infections. Objectives: To assess the prevalence and change in prevalence of early lower airway infections in a modern cohort of children with CF. Methods: All infants diagnosed with CF after newborn screening, participating in the AREST-CF cohort study between 2000-2018, were included. Participants prospectively underwent bronchoalveolar lavage (BAL) at 3-6 months,1 year and annually up to 6 years of age. Lower airway infection prevalence was described. Changes in prevalence patterns were assessed longitudinally using generalized estimating equations controlling for age and repeated visits. Measurements and Main Results: A total of 380 infants underwent 1,759 BALs. The overall prevalence and median age of first acquisition of the most common infections were: S. aureus 11%, 2.5yrs, P. aeruginosa 8%, 2.4yrs, Aspergillus species 11%, 3.2yrs, H. influenzae 9%, 3.1yrs. During the study, a significant decrease in prevalence of P. aeruginosa (p<0.001) and S. aureus (p<0.001) was observed with significant change towards more aggressive treatment. Prevalence of Aspergillus infections did not significantly change (p=0.669). Conclusion: Aspergillus species and P. aeruginosa are commonly present in the lower airways from infancy. The decrease in prevalence of P. aeruginosa and S. aureus since 2000, coinciding with more aggressive therapeutic approach, has resulted in Aspergillus becoming the most commonly isolated pathogen in young children. Further research is warranted to understand the implication of these findings.

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“…Inflammatory markers, neutrophil count, interleukin-8 and neutrophil elastase were measured in the pooled second and third BALF aspirates, as previously described [21]. Expression of MMP-1, MMP-2, MMP-7, MMP-9, TIMP-1 and TIMP-2 were measured using sandwich ELISA (RayBiotech, Norcross, GA, USA) (detection limits 24 pg·mL , respectively) in the pooled aspirate.…”

Section: Balf Processingmentioning

confidence: 99%

Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis

et al. 2015

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Neutrophil elastase is the most significant predictor of bronchiectasis in early-life cystic fibrosis; however, the causal link between neutrophil elastase and airway damage is not well understood. Matrix metalloproteinases (MMPs) play a crucial role in extracellular matrix modelling and are activated by neutrophil elastase. The aim of this study was to assess if MMP activation positively correlates with neutrophil elastase activity, disease severity and bronchiectasis in young children with cystic fibrosis.Total MMP-1, MMP-2, MMP-7, MMP-9, tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-1 levels were measured in bronchoalveolar lavage fluid collected from young children with cystic fibrosis during annual clinical assessment. Active/pro-enzyme ratio of MMP-9 was determined by gelatin zymography. Annual chest computed tomography imaging was scored for bronchiectasis.A higher MMP-9/TIMP-1 ratio was associated with free neutrophil elastase activity. In contrast, MMP-2/TIMP-2 ratio decreased and MMP-1 and MMP-7 were not detected in the majority of samples. Ratio of active/pro-enzyme MMP-9 was also higher in the presence of free neutrophil elastase activity, but not infection. Across the study cohort, both MMP-9/TIMP-1 and active MMP-9 were associated with progression of bronchiectasis.Both MMP-9/TIMP-1 and active MMP-9 increased with free neutrophil elastase and were associated with bronchiectasis, further demonstrating that free neutrophil elastase activity should be considered an important precursor to cystic fibrosis structural disease. @ERSpublications In young children with CF, activation of MMP-9 by free NE may provide one mechanism driving structural lung disease http://ow.ly/KArB5

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Stability of interleukin 8 and neutrophil elastase in bronchoalveolar lavage fluid following long-term storage

Abstract: Our data support the stability of IL-8 in BAL stored at -80 ° C for prolonged periods. NE in BAL decreases with storage and should be assayed as soon as practical after collection.

Cited by 12 publications

References 18 publications

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Changing Prevalence of Lower Airway Infections in Young Children with Cystic Fibrosis

Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis

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