Stability of ionic complexes prepared from plasmid DNA and self-aggregated chitosan nanoparticles

Lee, Kuen Yong

doi:10.1007/bf03218494

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…Spherical polymer nanoparticles have attracted considerable attention because of their unique properties different from those of the bulk materials (e.g, electrical, optical, thermal, mechanical and chemical characteristics). [9][10][11][12] To date, diverse synthetic ways to prepare polymer nanoparticles have been continuously developed. In particular, microemulsion polymerization has become one of the most extensively used methods.…”

Section: Polymer Nanostructuresmentioning

confidence: 99%

Versatile strategies for fabricating polymer nanomaterials with controlled size and morphology

et al. 2008

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The development of reliable synthetic routes to polymer nanomaterials with well-defined size and morphology is a critical research topic in contemporary materials science. The ability to generate nanometer-sized polymer materials can offer unprecedented, interesting insights into the physical and chemical properties of the corresponding materials. In addition, control over shape and geometry of polymer nanoparticles affords versatile polymer nanostructures, encompassing nanospheres, core-shell nanoparticles, hollow nanoparticles, nanorods/ fibers, nanotubes, and nanoporous materials. This review summarizes a diverse range of synthetic methods (broadly, hard template synthesis, soft template synthesis, and template-free synthesis) for fabricating polymer nanomaterials. The basic concepts and significant issues with respect to the synthetic strategies and tools are briefly introduced, and the examples of some of the outstanding research are highlighted. Our aim is to present a comprehensive review of research activities that concentrate on fabrication of various kinds of polymer nanoparticles.

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Section: Polymer Nanostructuresmentioning

confidence: 99%

Versatile strategies for fabricating polymer nanomaterials with controlled size and morphology

et al. 2008

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“…Especially chitosan has recently been studied as a novel carrier material in drug or gene delivery systems. [1][2][3][4][5][6][7][8][9][10] But in such applications, chitosan has shown some inherent limitations of, for instance, the lack of water solubility 11 and the need of linker for higher drug loading. Various chemical modifications of chitosans have thus been adopted in order to widen their uses in various medical applications.…”

Section: Introductionmentioning

confidence: 99%

Regioselective succinylation and gelation behavior of glycol chitosan

et al. 2008

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Chitosan is normally acylated and subsequently conjugated with drugs for biomedical applications. This study examined the relationship between the succinylation and gelation behaviors of glycol chitosan. Glycol chitosan was acylated with succinic anhydride under a wide variety of reaction conditions, such as different molar ratios of succinic anhydride to glucosamine, different methanol content in the reaction media, and different reaction temperatures. Among these reaction parameters, the methanol content in the solvent played an important role in determining the regioseletive succinylating site. N-succinylation and N-N cross-linking occurred regardless of the reaction conditions. However, O-succinylation was observed under specific conditions, i.e. a methanol content > 0.6 (v/v) and a reaction temperature > 25 o C. O-succinylation accelerated the N-O cross-linking of glycol chitosan, and led to gelation. The N-succinylated glycol chitosans were water-soluble, whereas the N-and O-succinylated glycol chitosans formed a gel. These physico-chemical structural differences in the succinylated glycol chitosans would definitely influence subsequent drug-conjugation reactions and consequently the drug loading and release kinetics.

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“…11 For amplification or multiplication of FA effects on a molecular level, a single polymeric chain may be conjugated with multiple ligands to enhance the binding affinity of the targeting moiety. 12 Polyvalent interactions can be much stronger than monovalent interactions, and they can provide the basis for mechanisms of both agonizing and antagonizing biological interactions that are fundamentally different from those available in monovalent systems. [13][14][15] Especially, ligand-receptor interactions are important in cancer targeting to create high local concentrations of drugs around tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of folic acid linked poly(L-glutamate) nanoparticles for cancer targeting

Lee

2006

Macromol. Res.

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Nanoparticles of Poly(L-glutamic acid) (PG) conjugated to the anticancer drug paclitaxel and targeted moiety folic acid (FA) were synthesized and characterized in vitro. The nanoparticles were designed to take advantage of FA targeting to folate receptor (FR) positive cancer cells. The chemical composition of the conjugate was characterized by 1 H-NMR, FTIR and UV/vis spectroscopy. The selective cytotoxicity of the FA-PG-paclitaxel conjugates was evaluated in FR positive cancer cells. The interaction of the conjugate was visualized by fluorescence microscopy with results confirming the successful preparation of the conjugate and the production of nanoparticles of about 200-300 nm in diameter. The amount of paclitaxel conjugated to FA-PG was 25% by weight. Cellular uptake of the conjugate was FA dependent, and the conjugate uptake was mediated specifically by the folate receptor. These results demonstrate the improved selective toxicity and effective delivery of an anticancer drug into FR bearing cells in vitro.

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Stability of ionic complexes prepared from plasmid DNA and self-aggregated chitosan nanoparticles

Cited by 8 publications

References 17 publications

Versatile strategies for fabricating polymer nanomaterials with controlled size and morphology

Versatile strategies for fabricating polymer nanomaterials with controlled size and morphology

Regioselective succinylation and gelation behavior of glycol chitosan

Preparation and characterization of folic acid linked poly(L-glutamate) nanoparticles for cancer targeting

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