Stability of lactate dehydrogenase in plasma at different temperatures: post-analytical storage

López, Marta Gómez; Roca, N. Rodríguez; Velasco, Manuela Simón; Martín, María José Alcaide; Soto, Antonio Buño; Rioja, Rubén Gómez

doi:10.1515/almed-2020-0077

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

2020

DOI: 10.1515/almed-2020-0077

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Stability of lactate dehydrogenase in plasma at different temperatures: post-analytical storage

Marta Gómez López

N. Rodríguez Roca

Manuela Simón Velasco

et al.

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2024

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Cited by 2 publications

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The stability of 65 biochemistry analytes in plasma, serum, and whole blood

Zhou,

Fabros,

Lam

et al. 2024

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives The pre-analytical stability of various biochemical analytes requires careful consideration, as it can lead to the release of erroneous laboratory results. There is currently significant variability in the literature regarding the pre-analytical stability of various analytes. The aim of this study was to determine the pre-analytical stability of 65 analytes in whole blood, serum and plasma using a standardized approach. Methods Blood samples were collected from 30 healthy volunteers (10 volunteers per analyte) into five vacutainers; either SST, Li-heparin, K2-EDTA, or Na-fluoride/K-oxalate. Several conditions were tested, including delayed centrifugation with storage of whole blood at room temperature (RT) for 8 h, delayed centrifugation with storage of whole blood at RT or 4 °C for 24 h, and immediate centrifugation with storage of plasma or serum at RT for 24 h. Percent deviation (% PD) from baseline was calculated for each analyte and compared to the maximum permissible instability (MPI) derived from intra- and inter-individual biological variation. Results The majority of the analytes evaluated remained stable across all vacutainer types, temperatures, and timepoints tested. Glucose, potassium, and aspartate aminotransferase, among others, were significantly impacted by delayed centrifugation, having been found to be unstable in whole blood specimens stored at room temperature for 8 h. Conclusions The data presented provides insight into the pre-analytical variables that impact the stability of routine biochemical analytes. This study may help to reduce the frequency of erroneous laboratory results released due to exceeded stability and reduce unnecessary repeat phlebotomy for analytes that remain stable despite delayed processing.

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The stability of 65 biochemistry analytes in plasma, serum, and whole blood

Zhou,

Fabros,

Lam

et al. 2024

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

show abstract

Add-on testing: stability assessment of 63 biochemical analytes in centrifuged and capped samples stored at 16 °C

Nielsen,

Ladefoged,

Madsen

2024

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives Integration of add-on testing in high-scale automated clinical laboratories constitute a valuable instrument not only for the clinicians and the general patient care, but also for the laboratory itself. Knowledge on sample quality and analytical stability upon storage is necessary to be able to offer add-on testing. The objectives of this study were to examine the analytical stability of 63 biochemical analytes in plasma and urine samples stored at 16 °C. Methods Samples were collected by professional laboratory technicians, analyzed at automated analyzers and stored in their primary, capped tube without separator for 10, 12, 16, 20 or 24 h at 16 °C. Stability was assessed by inspecting mean concentration of samples at baseline and examining if (A) mean concentration over time violated limits of bias, or if (B) individual sample concentrations violated limits of total error. Results The majority of the 63 analytes were stable for up to 24 h of storage. Few of the analytes were only suitable for add-on testing for 4, 6, 10, 12, 16 or 20 h of storage. One analyte, P-lactate dehydrogenase, was not found suitable for add-on testing when stored at 16 °C. Conclusions Due to the increasing number of intelligent solutions for high-scale clinical laboratories, add-on testing has come to stay. Loss of stability could not be demonstrated for the majority of analytes after 10, 12, 16, 20 or 24 h of storage. This feature of analytical stability suggests that add-on testing is an acceptable tool for these analytes.

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Stability of lactate dehydrogenase in plasma at different temperatures: post-analytical storage

Cited by 2 publications

References 5 publications

The stability of 65 biochemistry analytes in plasma, serum, and whole blood

The stability of 65 biochemistry analytes in plasma, serum, and whole blood

Add-on testing: stability assessment of 63 biochemical analytes in centrifuged and capped samples stored at 16 °C

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Product

Resources

About

Stability of lactate dehydrogenase in plasma at different temperatures: post-analytical storage

Cited by 2 publications

References 5 publications

The stability of 65 biochemistry analytes in plasma, serum, and whole blood

The stability of 65 biochemistry analytes in plasma, serum, and whole blood

Add-on testing: stability assessment of 63 biochemical analytes in centrifuged and capped samples stored at 16 °C

Contact Info

Product

Resources

About

Add-on testing: stability assessment of 63 biochemical analytes in centrifuged and capped samples stored at 16 °C