Tomato (Solanum lycopersicum L.) seeds and skins, which are the wastes of the tomato processing industry, are often regarded as animal feed in everyday life. In this study, tomato seed oil was obtained by Soxhlet extractor. The oil yield of tomato seeds was approximately 20.8%. The oils in the transparent, amber and opaque glass bottles were exposed to sunlight from June to September. The sample intake was made every 20 days and this procedure was repeated 4 times. Each sample was analyzed for fatty acid composition in gas chromatography. The minimum and maximum rates of the major fatty acids in all of the oil samples were 54.12-55.72%, 21.68-22.35%, 11.92-12.55%, 6.18-6.63% and 2.39-2.62% for linoleic (C18:2n6c), oleic (C18:1n9c), palmitic (C16:0), stearic (C18:0) and α-linolenic acids (C18:3n3), respectively. The results showed that there was no highly variation between the fatty acid rates in all samples.