Stability of oxidative phosphorylation and structural changes of mitochondria in ischemic rat liver

Boime, Irving; Smith, Ellen E.; Hunter, F. Edmund

doi:10.1016/0003-9861(68)90079-9

Cited by 33 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, our answers to the questions raised in the introduction are: a) In the initial stage of ischaemia the decrease in aldolase activity indicates a breakdown of aerobic glycolysis and the simultaneous start of lactic acid fermentation.These observations are in agreement with the findings of de Duve, Hohorst, Boime, Brosnan and others [9,14,4,5,3,35].…”

Section: Discussionsupporting

confidence: 94%

“…Changes in mitochondrial respiration has been described by Boime and Daniel [4,7,8], and that, about the macroerg phosphates and tissue ions, by De Duve and Brosnan [9,5]. The results are, more contradictory as on the above bases; a 10 to 90 min period was found to be the interval, in which the irreversible lesion of the liver develops.…”

Section: Discussionmentioning

confidence: 72%

“…Farkouh and Eisenbach agree that the inorganic phosphorus level rises during ischaemia. Almost all of these authors mention an increase in the lactate content of the tissue [4,7,10,11,35].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of ischaemia on the enzyme activity of the hepatic tissue

1977

View full text Add to dashboard Cite

The lactate and pyruvate levels, as well as acid and alkaline phosphatase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, glutaminic acid-oxalacetic acid transaminase and aldolase levels of rat liver homogenizates were measured at 24 degrees C and 38 degrees C during 120 min ischaemia from 0 to the 120th min. With the exception of transaminase and aldolase, the other enzymes were also enzyme-histochemically studied. The early lesions of the liver can be detected, both the quantitative laboratory tests and enzyme histochemical studies. The deviations from normal, observed at 24 degrees C between the 60th and 100th min, and at 38 degrees C between the 30th and 60th min, might be signs of irreversible lesions. Fractionated study of the liver homogenizate improves the assessability of enzyme determinations. In the course of "warm" ischaemia, the liver lysosomal lesions are early symptoms. Parallel to the breakdown of aerobic glycolysis lactic acid, fermentation, and an active pentose phosphate cycle can be detected. Quantitative testing of the liver homogenizate and enzyme histochemical observation of the hepatic tissue, might be a suitable method for the assessment of ischaemic liver lesions.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 72%

See 1 more Smart Citation

Effect of ischaemia on the enzyme activity of the hepatic tissue

1977

View full text Add to dashboard Cite

show abstract

“…As a result, dBSA came to be widely included during both preparation of mitochondria and in the media used for their study, usually without assessment of function in the absence of dBSA, thus obscuring the contribution of NEFA to pathological states. As in the present work, early studies of mitochondria isolated from ischemic liver in the absence of dBSA documented substantial mitochondrial dysfunction that could be entirely reversed by inclusion of dBSA (6,7). Mitochondria isolated in the absence of dBSA from ischemic heart displayed increased rates of proton leak that were corrected by dBSA (9).…”

Section: Discussionmentioning

confidence: 64%

Accumulation of nonesterified fatty acids causes the sustained energetic deficit in kidney proximal tubules after hypoxia-reoxygenation

Feldkamp¹,

Kribben²,

Roeser³

et al. 2006

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

Kidney proximal tubules exhibit decreased ATP and reduced, but not absent, mitochondrial membrane potential (Deltapsi(m)) during reoxygenation after severe hypoxia. This energetic deficit, which plays a pivotal role in overall cellular recovery, cannot be explained by loss of mitochondrial membrane integrity, decreased electron transport, or compromised F1F0-ATPase and adenine nucleotide translocase activities. Addition of oleate to permeabilized tubules produced concentration-dependent decreases of Deltapsi(m) measured by safranin O uptake (threshold for oleate = 0.25 microM, 1.6 nmol/mg protein; maximal effect = 4 microM, 26 nmol/mg) that were reversed by delipidated BSA (dBSA). Cell nonesterified fatty acid (NEFA) levels increased from <1 to 17.4 nmol/mg protein during 60- min hypoxia and remained elevated at 7.6 nmol/mg after 60 min reoxygenation, at which time ATP had recovered to only 10% of control values. Safranin O uptake in reoxygenated tubules, which was decreased 85% after 60-min hypoxia, was normalized by dBSA, which improved ATP synthesis as well. dBSA also almost completely normalized Deltapsi(m) when the duration of hypoxia was increased to 120 min. In intact tubules, the protective substrate combination of alpha-ketoglutarate + malate (alpha-KG/MAL) increased ATP three- to fourfold, limited NEFA accumulation during hypoxia by 50%, and lowered NEFA during reoxygenation. Notably, dBSA also improved ATP recovery when added to intact tubules during reoxygenation and was additive to the effect of alpha-KG/MAL. We conclude that NEFA overload is the primary cause of energetic failure of reoxygenated proximal tubules and lowering NEFA substantially contributes to the benefit from supplementation with alpha-KG/MAL.

show abstract

“…Surgical operations were performed under anesthesia (33 mg of sodium pentobarbital/kg) . Mitochondria were isolated from samples of liver tissue (about 3 g ) , as described by Boime et al (4) and were subjected (3 times) to freezing and thawing before 'measuring enzyme activities. LM-HBDH ( S ) , protein in mitochondria1 suspensions ( 6 ) , blood sugar (7), total ketone bodies (8,9), acetoacetate (8,9 ) , and free fatty acids in plasma (10) were determined as described in the respective references.…”

mentioning

confidence: 99%

Decreased Activity of 3-Hydroxybutyrate Dehydrogenase in Diabetic Liver Mitochondria

Roldán

Castillo

Boveris

et al. 1971

Experimental Biology and Medicine

View full text Add to dashboard Cite

According to Boveris et al. ( l ) , pancreatectomy decreased the rate of oxidation of 3-hydroxybutyrate by isolated rat liver mitochondria. This observation was consistent with a previous one by Schaefer and Nagel ( 2 ) showing that insulin administration to normal rats increased the activity of 3-hydroxybutyrate dehydrogenase (D-3-hydroxybutyrate : NAD oxidoreductase; EC 1.1.1.30) in liver mitochondria (LM-HBDH). The purpose of the present study was first, to confirm the eventual diminution of LM-HBDH after pancreatectomy, which was implicit in Boveris et al. (1) result, and second, to establish whether the growth hormone secretion of the hypophysis affects the activity of LM-HBDH in parallel with the action on carbohydrate metabolism, antagonistic to that of insulin [Houssay ( 3 ) ] . On these grounds, we investigated the effect of pancreatectomy, hypophysectomy, insulin, and growth hormone on LM-HBDH in the dog, The metabolic state of animals was monitored by measuring blood sugar, ketone bodies, and free fatty acids in plasma.Materials and Methods. Male and female dogs weighing 8-12 kg were used. The animals were separated into six experimental groups namely, (I) normal dogs after laparotomy ( N ) ; (11) depancreatized dogs, 2 days after operation (P); (111) same as (P) treated with insulin for 3 days after pan-

show abstract

Stability of oxidative phosphorylation and structural changes of mitochondria in ischemic rat liver

Cited by 33 publications

References 24 publications

Effect of ischaemia on the enzyme activity of the hepatic tissue

Effect of ischaemia on the enzyme activity of the hepatic tissue

Accumulation of nonesterified fatty acids causes the sustained energetic deficit in kidney proximal tubules after hypoxia-reoxygenation

Decreased Activity of 3-Hydroxybutyrate Dehydrogenase in Diabetic Liver Mitochondria

Contact Info

Product

Resources

About