Stability of piperacillin sodium in the presence of tazobactam sodium in 5% dextrose and normal saline injections

Mathew, Mary; Gupta, V. Das; Bethea, C.

doi:10.1111/j.1365-2710.1994.tb00700.x

Cited by 12 publications

(6 citation statements)

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“…In the latter cases, we used the MIC test strip and the SensiTitre results were confirmed. These results suggest that the majority of issues for both methods were represented by piperacillin/tazobactam, which is known to be unstable [ 31 ]. Details of AST results for each antibiotic are presented in Table 6 .…”

Section: Resultsmentioning

confidence: 99%

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Giordano

Piccoli

Brucculeri

et al. 2018

BioMed Research International

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Rapid identification of bloodstream pathogens by MALDI-TOF MS and the recently introduced rapid antimicrobial susceptibility testing (rAST) directly from positive blood cultures allow clinicians to promptly achieve a targeted therapy, especially for multidrug resistant microorganisms. In the present study, we propose a comparison between phenotypical rASTs performed in light-scattering technology (Alfred 60AST, Alifax®) and fluorescence in situ hybridization (Pheno™, Accelerate) directly from positive blood cultures, providing results in 4–7 hours. Blood samples from 67 patients admitted to the Azienda Ospedaliero-Universitaria Pisana were analyzed. After the direct MALDI-TOF MS identification, the rAST was performed at the same time both on Alfred 60AST and Pheno. Alfred 60AST provided qualitative results, interpreted in terms of clinical categories (SIR). Pheno provided identification and MIC values for each antibiotic tested. Results were compared to the broth microdilution assay (SensiTitre™, Thermo Fisher Scientific), according to EUCAST rules. Using Alfred 60AST, an agreement was reached, 91.1% for Gram-negative and 95.7% for Gram-positive bacteria, while using Pheno, the agreement was 90.6% for Gram-negative and 100% for Gram-positive bacteria. Both methods provided reliable results; Alfred 60AST combined with MALDI-TOF MS proved itself faster and cheaper. Pheno provided identification and MIC determination in a single test and, although more expensive, may be useful whenever MIC value is necessary and where MALDI-TOF MS is not present.

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Section: Resultsmentioning

confidence: 99%

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Giordano

Piccoli

Brucculeri

et al. 2018

BioMed Research International

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“…Administration by continuous or intermittent infusion was at the discretion of the treating physician. Patients treated by intermittent infusion received PIP/TAZ 4/0.5 g infused over 30 min every 6 h. Those treated by continuous infusion received a loading dose of PIP/TAZ 4/0.5 g over 30 min, followed by 4/0.5 g infused over 360 min every 6 h. Once dissolved, PIP/TAZ remains stable for 24-120 h at room temperature [12][13][14]. However, in our UCI new intravenous admixtures were prepared every 6 h. Each dose of PIP/TAZ was diluted in 100 mL of 0.9% NaCl.…”

Section: Methodsmentioning

confidence: 99%

Clinical cure of ventilator-associated pneumonia treated with piperacillin/tazobactam administered by continuous or intermittent infusion

Lorente¹,

Jiménez²,

Martı́n³

et al. 2009

International Journal of Antimicrobial Agents

121

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“…As Mathew et al (1994) reported that tazobactam appeared to have a slight adverse effect on the stability of piperacillin, a stability assay of piperacillin in plasma in the presence of tazobactam was carried out. Table 5 shows the stability data corresponding to 2 months, as all the results were satisfactory and only the latest ones are presented.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Quantitation and stability of piperacillin and tazobactam in plasma and ultrafiltrate from patients undergoing continuous venovenous hemofiltration by HPLC

Arzuaga

Isla

Rodríguez-Gascón

et al. 2005

Biomedical Chromatography

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Simple and reproducible HPLC methods for the determination of piperacillin and tazobactam have been developed and a complete stability study carried out. The method for piperacillin plasma samples consisted of protein precipitation with methanol using penicillin G as internal standard. No sample preparation was needed for ultrafiltrate samples. Tazobactam sample preparation involved protein precipitation with acetonitrile and the removal of lipids with dichloromethane. Piperacillin separation was performed on a microBondapack C(18) column (300 x 3.9, 10 microm) and tazobactam on a Novapack C(18) column (150 x 3.9, 4 microm) with UV detection set at 229 and 225 nm, respectively. The mobile phase consisted of phosphate buffer-acetonitrile, delivered at 1.5 mL[sol ]min. Calibration curves determination coefficients were >or=0.999 and response factors CV% < 5%. Intra- and inter-assay precision and accuracy of the quality control and limit of quantification were satisfactory. Plasma and ultrafiltrate samples were stable at -20 and -80 degrees C for 2 months and after three freeze-thaw cycles. In the chromatographic rack, tazobactam ultrafiltrate samples were stable for 24 h and plasma samples for 12 h, piperacillin ultrafiltrate samples for 8 h, but plasma samples for only 4 h. Storage of piperacillin samples at 4 degrees C until analysis is recommended. Piperacillin was stable in the presence of tazobactam.

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Stability of piperacillin sodium in the presence of tazobactam sodium in 5% dextrose and normal saline injections

Cited by 12 publications

References 0 publications

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

A Prospective Evaluation of Two Rapid Phenotypical Antimicrobial Susceptibility Technologies for the Diagnostic Stewardship of Sepsis

Clinical cure of ventilator-associated pneumonia treated with piperacillin/tazobactam administered by continuous or intermittent infusion

Quantitation and stability of piperacillin and tazobactam in plasma and ultrafiltrate from patients undergoing continuous venovenous hemofiltration by HPLC

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