Stability of plant vacuolar membranes under the conditions of osmotic stress and influence of redox agents

Nurminsky, V. N.; Озолина, Н. В.; Nesterkina, I. S.; Колесникова, Е. В.; Korzun, A. M.; Chernyshov, Michael Yu.; Tikhonov, N. V.; Tarkov, M. S.; Salyaev, R. K.

doi:10.1134/s1990747811020048

Cited by 4 publications

(3 citation statements)

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“…More intensive degradation of the vacuoles took place under hypoosmotic stress, when the half-life was 22% of the check result. Under hyperosmotic stress it was 72% (Nurminsky et al 2011).…”

Section: Resultsmentioning

confidence: 98%

“…The content of diene conjugates was measured by the method (Vladimirov and Archakov 1972). The effect of osmotic stress upon the membrane stability was assessed with the aid of our installation, which allowed us to obtain series of computer images [time-lapse video recording, frame rate 0.1 min −1 (1 frame per 10 min)] reflecting the dynamics of the process of vacuolar destruction (Nurminsky et al 2011). We used computer data processing and plotted the dependence of the number of retained vacuoles (%) with respect to time and assessed the half-life (T 1/2 ) of the isolated vacuoles, during which 50% of the vacuoles are destroyed.…”

Section: Stress Conditionsmentioning

confidence: 99%

“…The H + -ATPase activity was measured by quantifying the inorganic phosphate (P i ) released from ATP. The content of P i released was measured by the Lowry-Lawrence method modified by Skulachev (Nikulina 1965) and was expressed as a ratio of the concentration of P i to the concentration of proteins per 1 h (µmol P i (mg of protein) −1 h −1 ). The mass of proteins was assessed by Bradford's method (1976) (Bradford 1976) with the use of bovine serum albumin as the standard.…”

Section: Isolation Of Membrane Materialsmentioning

confidence: 99%

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Role of Plasmalemma Microdomains (Rafts) in Protection of the Plant Cell Under Osmotic Stress

et al. 2021

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Lipid-protein microdomains (presumably rafts) of the plasmalemma isolated from the beetroots subjected to hyperosmotic stress and hypoosmotic stress were studied. In these microdomains, the variations in the composition of total lipids, sterols, and fatty acids were observed. These variations differed under hypo-and hyperosmotic types of stress. We presumed that such variations were bound up with different strategies, which are probably related to protecting the cell from osmotic stress. One of the protection tendencies might be related, in our opinion, to credible growth of the content of such lipids as sterols and sterol esters, which are considered as raft-forming. Under osmotic stress, these lipids can contribute to the formation of both new raft structures and new membrane contacts of plasmalemma with intracellular organelles. Another protection tendency may be bound up with the redistribution of membrane phospholipids and phosphoglycerolipids possibly to stabilize the membrane's lamellar structure, which is ensured by credible growth of the content of such lipids as phosphatidylcholines, phosphatidylinositols, and digalactosyldiacylglycerol. The participation of lipid-protein microdomains in the adaptive mechanisms of plant cells may, in our opinion, also be bound up with the redistribution of membrane sterols, which (redistribution) in a number of variants may provoke credible growth in the content of cholesterol or "anti-stress" sterols (campesterol and stigmasterol). So, according to our results, the variations in the content of the plasmalemma lipid-protein microdomains take place under osmotic stress. These variations may influence the functioning of plasmalemma and take part in the adaptive mechanisms of plant cells.

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Section: Resultsmentioning

confidence: 98%

Section: Stress Conditionsmentioning

confidence: 99%