Stability of plasma electrolytes in Barricor and PST II tubes under different storage conditions

Balbás, Luis Alfredo Bautista; Amaro, Marta Segovia; Rioja, Rubén Gómez; Martín, María José Alcaide; Soto, Antonio Buño

doi:10.11613/bm.2017.024

Cited by 15 publications

(5 citation statements)

References 6 publications

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“…However, higher concentrations of K in the tubes with a barrier than in the barrier-free LiH tubes may be due to prolonged cell contact, but further investigation is needed to explain the underlying cause. In a recent study on sample stability, the basal K concentrations in Barricor plasma were lower than the K concentrations in Plasma Separator (PST) plasma in contrast to these findings ( 20 ). Therefore, it is necessary to evaluate these Barricor tubes in different settings to clarify the net effects.…”

Section: Discussionmentioning

confidence: 88%

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

et al. 2017

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IntroductionAlthough serum-providing blood tubes with a barrier are still widely used due to their significant advantages, the use of blood tubes with a barrier to provide plasma is becoming widespread. We compared 22 analytes in a BD Vacutainer® Barricor LH Plasma tube for local clinical validation of this new lithium heparin tube with a barrier.Materials and methodsSamples from 44 volunteers were collected in different tubes (Becton Dickinson and Company): Z tube without additive (reference), clot-activator tube with gel (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with barrier (Barricor). Analyte concentrations in different tubes were compared with the reference tube. All tubes were also evaluated according to additional testing (different centrifugation durations, blood-sampling techniques and individual differences).ResultsAspartate aminotransferase (AST), glucose (Glc), potassium (K), lactate dehydrogenase (LD), sodium (Na), and total protein (TP) had a significant bias in Barricor (9.19%, - 3.24%, - 4.88%, 21.60%, - 0.40%, 5.03%, respectively) relative to the reference tube. There was no statistical difference between different centrifugation durations and individual differences for AST, K and LD in LiH and/or Barricor (P > 0.05). There was a significant bias for LD between LiH and Barricor in terms of blood-sampling techniques (21.2% and 12.4%, respectively).ConclusionsRecently, the use of plasma has become prominent due to some of its advantages. In this study, plasma AST, K, LD, Glc and TP levels in Barricor were clinically different in comparison to serum. The results of additional tests showed that higher levels of LD in Barricor did not result from haemolysis, and they might be related to other factors including number of platelets, cellular fragility, or functional environment.

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Section: Discussionmentioning

confidence: 88%

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

et al. 2017

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“…According to the manufacturer, the quality of plasma is improved, the centrifugation time is shorter (3 min vs. 10 min with BD Vacutainer® lithium heparin tubes) and post centrifugation analyte concentrations are more stable in samples that have been stored in the primary tube. At this time, some studies have been published to verify if these tubes are an effective alternative to gel barrier tubes ( 3 - 5 ). Nevertheless, these publications concern mainly the correlations between results obtained using different tubes for biochemical parameters, particularly cardiac markers, without stability studies ( 3 , 4 ).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, these publications concern mainly the correlations between results obtained using different tubes for biochemical parameters, particularly cardiac markers, without stability studies ( 3 , 4 ). The one study which has investigated different storage conditions relates only to electrolytes ( 5 ). Given this lack of data, we aimed to compare results for nine biochemical tests from the BD Vacutainer® lithium heparin tubes vs. the BD Barricor™ plasma tubes and to determine plasma stability of analytes after centrifugation according to our routine laboratory practice.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Barricor™ vs. lithium heparin tubes for selected routine biochemical analytes and evaluation of post centrifugation stability

Dupuy¹,

Badiou²,

Daubin³

et al. 2018

Biochemia Medica

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IntroductionObtaining suitable results unaffected by pre- or postanalytical phases is pivotal for clinical chemistry service. We aimed comparison and stability of nine biochemical analytes after centrifugation using Barricor™ plasma tubes with mechanical separator vs standard Vacutainer® lithium heparin tubes.Materials and methodsWe collected samples on six healthy volunteers and nine patients from intensive care units into 6 mL plastic Vacutainer® lithium heparin tubes and 5.5 mL plastic Barricor™ plasma tubes. All tubes were centrifuged within 30 minutes after venipuncture. First, we compared results of nine biochemical analytes from lithium heparin tubes with Barricor™ tubes for each analyte using Passing-Bablok and Bland-Altman analyses. Second, we calculated the difference of analyte concentrations between baseline and time intervals in tubes stored at + 4 °C. Based on the total change limit we calculated the maximum allowable concentrations percentage change from baseline.ResultsThe majority of correlation coefficients were close to 0.99 indicating good correlation in the working range. Bland-Altman analyses showed an acceptable concordance for all analytes. In consequence, the Barricor™ tube might be an alternative to regular lithium heparin tube. Stability with this new generation tube is improved for eight analytes (except for aspartate aminotransferase) in comparison with regular lithium heparin tubes.ConclusionsBy using Barricor™ tubes and prompt centrifugation, supplemental analysis or re-analysis for eight analytes including alanine aminotransferase, alkaline phosphatase, C-reactive protein, high sensitivity troponin T, lactate dehydrogenase, NT-pro BNP, potassium and sodium could be performed within 72 h of specimen collection.

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“…As described in literature, serum displays a better sample stability compared to plasma ( 4 ). This is mostly attributed to blood cells remaining in plasma in higher concentration than in serum.…”

Section: Discussionmentioning

confidence: 86%

“…In recent years, only few BD Barricor tube evaluations were performed, mostly encompassing samples from healthy participants ( 4 , 7 , 13 - 15 ). Nevertheless, to the best of our knowledge, this is the first study evaluating BD Barricor tubes in relation to BD RST tubes in terms of analytes’ comparability and long-term stability of 25 analytes from in-patient samples.…”

Section: Discussionmentioning

confidence: 99%

Test results comparison and sample stability study

Mandić

Lukić

et al. 2020

Biochem. med. (Online)

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Introduction: The aim was to evaluate the BD Barricor tubes by comparison with the BD Rapid Serum Tubes (RST) through measuring 25 analytes and monitoring sample stability after 24 hours and 7 days. Materials and methods: Samples of 52 patients from different hospital departments were examined. Blood was collected in BD RST and BD Barricor tubes (Becton, Dickinson and Company, Franklin Lakes, USA). Analytes were measured by Beckman Coulter AU 480 (Beckman Coulter, Brea, USA), Dimension EXL (Siemens Healthcare Diagnostics, Newark, USA) and ARCHITECT i2000SR (Abbott Diagnostics, Lake Forest, USA). Between-tube comparison for each analyte was performed, along with testing analyte stability after storing samples at 4 °C. Results: BD Barricor tubes showed unacceptable bias compared to BD RST tubes for potassium (K) (- 4.5%) and total protein (4.4%). Analyte stability after 24 hours was acceptable in both tested tubes for most of analytes, except for glucose, aspartate aminotransferase (AST) and lactate dehydrogenase (LD) in BD Barricor and free triiodothyronine in BD RST sample tubes. Analyte stability after 7 days was unacceptable for sodium, K, calcium, creatine kinase isoenzyme MB, AST, LD and troponin I in both samples; additionally for glucose, alkaline phosphatase and albumin in BD Barricor. Conclusion: All analytes, except K and total protein, can be measured interchangeably in BD RST and BD Barricor tubes, applying the same reference intervals. For most of the analytes, sample re-analysis can be performed in both tubes after 24 hours and 7 days, although BD RST tubes show better 7-day analytes stability over BD Barricor tubes.

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Stability of plasma electrolytes in Barricor and PST II tubes under different storage conditions

Cited by 15 publications

References 6 publications

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

Comparison of Barricor™ vs. lithium heparin tubes for selected routine biochemical analytes and evaluation of post centrifugation stability

Test results comparison and sample stability study

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