Stability of proteins: Temperature, pressure and the role of the solvent

Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, and fruits, can transmit noroviruses. We inactivated feline calicivirus, a surrogate for the nonculturable norovirus, in cell culture medium and mineral water by heat and high hydrostatic pressure. Incubation at ambient pressure and 75°C for 2 min as well as treatment at 450 MPa and 15°C for 1 min inactivated more than 7 log 10 PFU of calicivirus per ml in cell culture medium or mineral water. The heat and pressure time-inactivation curves obtained with the calicivirus showed tailing in the logarithmic scale. Modeling by nth-order kinetics of the virus inactivation was successful in predicting the inactivation of the infective virus particles. The developed model enables the prediction of the calicivirus reduction in response to pressures up to 500 MPa, temperatures ranging from 5 to 75°C, and various treatment times. We suggest high pressure for processing of foods to reduce the health threat posed by noroviruses.

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“…MICROBIOL. (44). As a consequence, tertiary and quaternary protein structures are disrupted and the protein partly unfolds.…”

mentioning

confidence: 99%

Predictive Model for Inactivation of Feline Calicivirus, a Norovirus Surrogate, by Heat and High Hydrostatic Pressure

Buckow

Isbarn

Knorr

et al. 2008

Appl Environ Microbiol

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“…The volume changes, 0 V  , which broadly follow the published volume changes of protein unfolding (Scharnagl, Reif et al 2005), are generally greater for isolated (-51 to -71 ml/mol) than for membrane (-14 --39 ml/mol) samples. Both the pressure and volume features agree with the general notion that membranes protect and stabilize integral membrane proteins.…”

Section: Evaluation the B850 Chromophore-binding Hydrogen Bond Energiesmentioning

confidence: 63%

“…The phenomenological basis of protein stability against pressure is well established (Silva and Weber 1993;Boonyaratanakornkit, Park et al 2002;Scharnagl, Reif et al 2005;Meersman, Dobson et al 2006). In a minimalist version of thermodynamic modelling just two global protein states, native (N) and denatured (D), are assumed.…”

Section: Thermodynamic Aspects Of Protein Stability Against Pressurementioning

confidence: 99%

“…T o d a t e m o s t l y o n l y w a t e r -s o l u b l e g l o b u l a r p r o t e i n s h a v e b e e n s t u d i e d u n d e r h i g h pressures, with the result that functional protein structures cover just a narrow region in the pressure-temperature phase diagram close to physiological temperatures (Silva and Weber 1993;Boonyaratanakornkit, Park et al 2002;Scharnagl, Reif et al 2005;Meersman, Dobson et al 2006). The photosynthetic LH membrane complexes were first investigated under high pressure in (Freiberg, Ellervee et al 1993).…”

mentioning

confidence: 99%

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Estimating Hydrogen Bond Energy in Integral Membrane Chromoproteins by High Hydrostatic Pressure Optical Spectroscopy

Kangur¹,

Olsen²,

Hunter³

et al. 2012

Protein Structure

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“…Protein stability is directly correlated with the ability of water in the hydration layer to fluctuate among different equilibrium structure [19]. Viral vaccines are highly sensitive, respond differently to physical stresses, commonly it can be affected differently by solvents, pH, and ionic strength of extreme temperatures (heat/cold) [20].…”

Section: Preparation and Quality Control Of Tcalrv-bmentioning

confidence: 99%

Formulation, Efficacy and Immunogenicity Studies of a Liquid State Rabies Vaccine with Magnesium Chloride as Stabilizer

Selvaraj¹,

Rajendran²

2015

J Vaccines Vaccin

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Propagation of fixed rabies virus strain in vero cell line, ultra filtration of rabies viral harvest, betapropiolactone inactivation subsequent purification, total protein nitrogen quantification, Rapid Fluorescent focus inhibition test, MgCl 2 based liquid rabies vaccine formulation (TCALRV-B) and immune response analysis. The vero cell derived PV 11 rabies viral titers were found that 10 -5.3 , ultra filtrated, betapropiolactone inactivated and viral protein qualities were within the range (WHO). The total protein concentration and PN 2 was 577.6, 0.26 mg/ml respectively. The host cellular protein and residual cellular DNA was 16 ng/single human doses and below 100 pg/ml respectively it reveals within the limit. The RFFIT titer of the TCALRV-B possessed higher immune response (8 IU/ml) of rabies neutralizing antibodies on 14 th on 21 st day the titer was four fold increasing even after 90 th day it reveals its immunopotency. The TCALRV-B contained all the quality attributes to fulfill the regulatories although it contained potency, immunogenicity and safety as in vivo study shows lesser immunogenic during the booster doses. This may be due to the unadsorption of rabies viral proteins by MgCl 2 as adjuvant.

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Stability of proteins: Temperature, pressure and the role of the solvent

Cited by 165 publications

References 120 publications

Predictive Model for Inactivation of Feline Calicivirus, a Norovirus Surrogate, by Heat and High Hydrostatic Pressure

Predictive Model for Inactivation of Feline Calicivirus, a Norovirus Surrogate, by Heat and High Hydrostatic Pressure

Estimating Hydrogen Bond Energy in Integral Membrane Chromoproteins by High Hydrostatic Pressure Optical Spectroscopy

Formulation, Efficacy and Immunogenicity Studies of a Liquid State Rabies Vaccine with Magnesium Chloride as Stabilizer

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