Stability of solutions of antineoplastic agents during preparation and storage for in vitro assays

Bosanquet, Andrew G.

doi:10.1007/bf00451642

Cited by 16 publications

(6 citation statements)

References 94 publications

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“…The mean IDs0s (1 h/3 d) for ACNU (47.2/ 46.2/xg/ml) were more than twice that of BCNU (20.4/18.6/xg/ml). This is in agreement with the study conducted by Bosanquet [20]. In culture medium supplemented with FCS he found the half-life of ACNU was 17.7 min and of BCNU 15 rain.…”

Section: Discussionsupporting

confidence: 81%

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The MTT assay for chemosensitivity testing of human tumors of the central nervous system

et al. 1992

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The aim of this study was to optimize the experimental conditions of the MTT assay for primary cultures of human brain tumors. This assay is based on the mitochondrial reduction of MTT-(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) salt to formazan crystals by living cells. Formazan can be quantified spectrophotometrically. This assay measures the antimetabolic and, by using an adequate recovery period for the cells, also the antiproliferative effects of cytotoxic drugs. Our results suggest the following experimental design for its application as an chemosensitivity assay for human brain tumors: 1-h drug exposure followed by a seven days recovery period without drugs. Then tumor cells are incubated 4 hours with 1 mg MTT/ml and final absorbance readings are performed at 550 nm and 630 nm as test and reference wavelengths respectively. In this way, the assay seems to be a reliable and simple method for rapid chemosensitivity testing in human brain tumors.

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Section: Discussionsupporting

confidence: 81%

“…However, mitomycin C and mitoxantrone showed a significant increase of their in vitro cytotoxicity, almost to a factor of 5, by prolonging the time of incubation. Both drugs have half-lifes in excess of two weeks [20]. Additionally cell cycle specific sensitivity could play another important role.…”

Section: Discussionmentioning

confidence: 99%

The MTT assay for chemosensitivity testing of human tumors of the central nervous system

et al. 1992

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“…E. coli -derived ASNase is known to retain activity for two days at room temperature and seven days when refrigerated [19, 20]. A related form of ASNase (derived from Erwinia carotovora ) was shown to lose activity after freezing [12].…”

Section: Discussionmentioning

confidence: 99%

E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic ActivityIn Vitrofor Canine and Feline Lymphoma after Cold Storage

Wypij

Pondenis

2013

Veterinary Medicine International

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Background. L-asparaginase is effective in treating canine and feline lymphoma, however chemotherapy poses a significant financial cost to veterinary clients, limiting therapy for many pets. Single dose vials result in significant drug wastage, and drug shortages limit consistent availability for pets. Hypothesis. E. coli-derived asparaginase retains enzymatic and antineoplastic activity in canine and feline lymphoma cells after cold storage. Methods. E. coli-derived asparaginase was cold-stored: refrigeration (7–14 days) and freezing (14 days–six months, one to three freeze/thaw cycles). Enzymatic activity of asparaginase was measured via a modified asparagine assay. Effects of cold-stored asparaginase on cell proliferation and cytotoxicity were measured in feline (MYA-1, F1B) and canine (17–71, OSW) lymphoma cells. Results. Cold-stored E. coli-derived asparaginase retains antineoplastic activity in all four cell lines tested. Cold-stored E. coli-derived L-asparaginase depletes asparagine and retains enzymatic activity. Duration of refrigeration, duration of freezing, and number of freeze-thaw cycles have minimal effect on asparaginase enzyme activity. Conclusions and Clinical Importance. This study establishes a scientific basis for long-term cold storage of reconstituted E. coli-derived asparaginase that may result in better utilization of limited drug resources and improve financial feasibility of E. coli-derived asparaginase as a therapeutic option for pets with lymphoma.

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“…According to an extensive review of Bosanquet [25], AC-NU and streptozotocin, the drugs most closely related to MCNU, as well as BCNU are stable at -70 ° C, which has been the condition of storage in our experiments. Besides that, incubation of the cells was performed prior to plating on agar in order to avoid any interference of the drug with the agar layer [19].…”

Section: Discussionmentioning

confidence: 99%

Sensitivity of human malignant intracranial tumors against MCNU in vitro in comparison to ACNU and BCNU

Tonn¹,

Schönmayr²,

Kraemer³

1990

J Neuro-Oncol

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An in vitro chemosensitivity test was carried out in 50 specimen of human malignant intracranial tumors. Aim of the study was to evaluate the proportion of sensitivity against MCNU (Ranomustine) in comparison to ACNU and BCNU. 47 tests were evaluable. Mean viability of the specimen was 83.3 +/- 18.7%, mean plating efficiency was 0.068 +/- 0.051%. 9/47 settings revealed sensitivity against MCNU in vitro (ACNU: 10/47; BCNU: 16/46). There was no advantage of MCNU concerning age or sex of the patients. Brain metastases seemed to be slightly more frequent sensitive against MCNU than primary brain tumors. Cross resistance between ACNU, BCNU and MCNU was rather high. The results of this in vitro series do not encourage a clinical trial of MCNU as an alternative to the commonly used nitrosoureas.

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Stability of solutions of antineoplastic agents during preparation and storage for in vitro assays

Cited by 16 publications

References 94 publications

The MTT assay for chemosensitivity testing of human tumors of the central nervous system

The MTT assay for chemosensitivity testing of human tumors of the central nervous system

E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic ActivityIn Vitrofor Canine and Feline Lymphoma after Cold Storage

Sensitivity of human malignant intracranial tumors against MCNU in vitro in comparison to ACNU and BCNU

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