Stability of Tetrahydrocannabinols I

Garrett, Edward R.; Tsau, Josef

doi:10.1002/jps.2600631016

Cited by 34 publications

(9 citation statements)

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“…Δ 9 -Tetrahydrocannabinol (THC), the primary psychoactive cannabis constituent and predominant OF analyte after smoking, degrades when exposed to air (6), acid (7), increased temperature, and light (8-9) and also adsorbs readily to glass, plastic, and precipitant material (10). There are few data on cannabinoid stability in OF.…”

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confidence: 99%

Cannabinoid Stability in Authentic Oral Fluid after Controlled Cannabis Smoking

et al. 2012

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BACKGROUND Defining cannabinoid stability in authentic oral fluid (OF) is critically important for result interpretation. There are few published OF stability data, and of those available, all employed fortified synthetic OF solutions or elution buffers; none included authentic OF following controlled cannabis smoking. METHODS An expectorated OF pool and a pool of OF collected with Quantisal™ devices were prepared for each of 10 participants. Δ9-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) stability in each of 10 authentic expectorated and Quantisal-collected OF pools were determined after storage at 4 °C for 1 and 4 weeks and at −20 °C for 4 and 24 weeks. Results within ±20% of baseline concentrations analyzed within 24 h of collection were considered stable. RESULTS All Quantisal OF cannabinoid concentrations were stable for 1 week at 4 °C. After 4 weeks at 4 °C, as well as 4 and 24 weeks at −20 °C, THC was stable in 90%, 80%, and 80% and THCCOOH in 89%, 40%, and 50% of Quantisal samples, respectively. Cannabinoids in expectorated OF were less stable than in Quantisal samples when refrigerated or frozen. After 4 weeks at 4 and −20 °C, CBD and CBN were stable in 33%–100% of Quantisal and expectorated samples; by 24 weeks at −20 °C, CBD and CBN were stable in ≤44%. CONCLUSIONS Cannabinoid OF stability varied by analyte, collection method, and storage duration and temperature, and across participants. OF collection with a device containing an elution/stabilization buffer, sample storage at 4 °C, and analysis within 4 weeks is preferred to maximize result accuracy.

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confidence: 99%

Cannabinoid Stability in Authentic Oral Fluid after Controlled Cannabis Smoking

et al. 2012

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“…THC is a brown, oily resin that is viscous and sticky at ambient conditions and hardens upon refrigeration, suggesting that the glass transition temperature (T g ) is below 258C, necessitating its storage at relatively low temperatures for stability purposes. Indeed, T g of the drug was well below room temperature as determined by van Drooge et al 2 The stability of THC has been a subject of numerous investigations [3][4][5][6][7][8][9][10][11][12] and it has been reported that the drug is susceptible to decomposition by light, heat, and oxidation. Storage leads to a cumulative decrease in THC content through its oxidation to cannabinol (CBN), which is the most widely recognized thermo-oxidative degradation product of THC.…”

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confidence: 99%

Polymeric systems for amorphous Δ9-tetrahydrocannabinol produced by a hot-melt method. Part I: Chemical and thermal stability during processing

Munjal

Stodghill

ElSohly

et al. 2006

Journal of Pharmaceutical Sciences

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“…the reversed-phase HPLC system, and the eluate was collected over the range for a 51% acetonitrile-water solvent system that would collect both compounds. The collected fraction was dried under nitrogen in a water bath (50"), reconstituted in 50 fil of chloroform, and analyzed by GLC, using flame ionization as previously described (2,3). …”

Section: Minmentioning

confidence: 99%