Stability of valeriana-type iridoid glycosides from rhizomes of Nardostachys jatamansi and their protection against H2O2-induced oxidative stress in SH-SY5Y cells

Serum albumin exposure enhances cell invasiveness and paclitaxel resistance in human neuroblastoma cells, with attenuation by valeriana‐type iridoid glycosides

“…Previously, we have found a potent antioxidant capacity of six valeriana‐type iridoid glycosides, isolated from rhizomes of N. jatamansi 12 . Two compounds, patrisophoroside (a trivial name for patrinoside‐aglycone‐11‐ O ‐[ β ‐ D ‐glucopyranosyl‐(1 → 2)‐ β ‐ D ‐glucopyranoside) and patrinalloside (Figure 3A), were selected based on their antioxidant potency.…”

“…SB431542, a TGFβ receptor I inhibitor (Tocris Bioscience); LY294002, a PI3K inhibitor (Sigma‐Aldrich); and the iridoid glycosides, patrisophoroside and patrinalloside, obtained from N. jatamansi were used if applicable. One of the iridoid glycosides, patrisophoroside, is trivially named for patrinoside‐aglycone‐11‐ O ‐[ β ‐ d ‐glucopyranosyl‐(1 → 2)‐ β ‐ d ‐glucopyranoside that was recently isolated in the previous study 12 . Details on reagents used in the present study are in Table S1.…”

“…One of the iridoid glycosides, patrisophoroside, is trivially named for patrinoside-aglycone-11-O-[β-dglucopyranosyl-(1 → 2)β-d-glucopyranoside that was recently isolated in the previous study. 12 Details on reagents used in the present study are in Table S1.…”

“…This is accompanied by increases in the level of matrix metalloprotease (MMP) 9, the metastatic invasiveness of neuroblastoma cells, and resistance to an anti‐cancer agent, paclitaxel. Furthermore, valeriana‐type iridoid glycosides recently isolated from Nardostachys jatamansi that have been recently found to be potent antioxidants, 12 patrinoside‐aglycone‐11‐ O ‐[ β ‐ d ‐glucopyranosyl‐(1 → 2)‐ β ‐ d ‐glucopyranoside (herein trivially named as patrisophoroside) and patrinalloside, substantially inhibited such effects of albumin. These results suggest that exposure to serum albumin may expedite tumor progression in neuroblastoma cells, with potential therapeutic implications for patrisophoroside and patrinalloside.…”

Benzophenone Rhamnosides and Chromones from Hypericum seniawinii Maxim.

“…The compound (0.5 mg) was treated with 3 M hydrochloric acid (0.5 mL) at 90 °C for 2 h. After neutralization with 3 M ammonium hydroxide, the reactants were dried by evaporation of the solvent. l -cysteine methyl ester (0.5 mg) and pyridine (0.2 mL) were added, and then stirred at 60 °C for 1 h. Finally, phenyl isothiocyanate solution (0.5 mL) was added and stirred at 60 °C for 1 h. [ 27 ]. The residue of each sample was subjected to analytical HPLC (with MeCN/H 2 O (25:75,1.0 mL/min) using a C18 RP column (4.6 mm × 250 mm, 5 μm, Thermo Fisher Scientific Inc., Waltham, MA, USA).…”