Stability problems in the hydrogen production by dark fermentation: Possible causes and solutions

Castelló, Elena; Ferraz-Junior, Antonio Djalma Nunes; Andreani, Cristiane Lurdes; Anzola-Rojas, Mélida del Pilar; Borzacconi, Liliana; Buitrón, Germán; Carrillo‐Reyes, Julián; Gomes, Simone Damasceno; Maintinguer, Sandra Imaculada; Moreno‐Andrade, Iván; Palomo-Briones, Rodolfo; Razo-Flores, Elı́as; Schiappacasse-Dasati, María; Tapia-Venegas, Estela; Valdez‐Vazquez, Idania; Vesga-Baron, Alejandra; Zaiat, Marcelo; Etchebehere, Claudia

doi:10.1016/j.rser.2019.109602

Cited by 167 publications

(56 citation statements)

References 169 publications

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“…As it has been highlighted in a recent excellent review paper ( Castelló et al, 2020 ), hydrogen-producing microbial communities show (i) a relatively low diversity in comparison to methanogenic communities and (ii) tendencies to change, which is reflected in the instability of hydrogen production. It is thought that microbial communities of low diversity are less stable and have limited rebuilding capabilities.…”

Section: Discussionmentioning

confidence: 99%

“…Symbiotic interactions between bacteria resulting from proper proportions between bacteria are determined by (i) the source of microorganisms and the selection it undergoes; (ii) operating conditions such as bioreactor construction, packing material and substrate concentration that are crucial for the continuous, stable process of hydrogen production ( Castelló et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, a decrease of substrate concentration to 8.5 g COD of molasses/L inhibited the development of LAB in the microbial community (PBR2) that supports our current (PBR4) and previous ( Detman et al, 2020 ) observations that sucrose stimulates LAB in DF consortia. Other factors preventing biomass accumulation and overproduction of SCFAs or alcohols are the HRT and the working volume that, together with the proper substrate concentration, ensure effective substrate utilization and gas release ( Castelló et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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This study describes the dynamics and complexity of microbial communities producing hydrogen-rich fermentation gas from sugar-beet molasses in five packed-bed reactors (PBRs). The bioreactors constitute a part of a system producing hydrogen from the by-products of the sugar-beet industry that has been operating continuously in one of the Polish sugar factories. PBRs with different working volumes, packing materials, construction and inocula were tested. This study focused on analysis (based on 16S rRNA profiling and shotgun metagenomics sequencing) of the microbial communities selected in the PBRs under the conditions of high (>100 cm3/g COD of molasses) and low (<50 cm3/g COD of molasses) efficiencies of hydrogen production. The stability and efficiency of the hydrogen production are determined by the composition of dark fermentation microbial communities. The most striking difference between the tested samples is the ratio of hydrogen producers to lactic acid bacteria. The highest efficiency of hydrogen production (130–160 cm3/g COD of molasses) was achieved at the ratios of HPB to LAB ≈ 4:2.5 or 2.5:1 as determined by 16S rRNA sequencing or shotgun metagenomics sequencing, respectively. The most abundant Clostridium species were C. pasteurianum and C. tyrobutyricum. A multiple predominance of LAB over HPB (3:1–4:1) or clostridia over LAB (5:1–60:1) results in decreased hydrogen production. Inhibition of hydrogen production was illustrated by overproduction of short chain fatty acids and ethanol. Furthermore, concentration of ethanol might be a relevant marker or factor promoting a metabolic shift in the DF bioreactors processing carbohydrates from hydrogen-yielding toward lactic acid fermentation or solventogenic pathways. The novelty of this study is identifying a community balance between hydrogen producers and lactic acid bacteria for stable hydrogen producing systems. The balance stems from long-term selection of hydrogen-producing microbial community, operating conditions such as bioreactor construction, packing material, hydraulic retention time and substrate concentration. This finding is confirmed by additional analysis of the proportions between HPB and LAB in dark fermentation bioreactors from other studies. The results contribute to the advance of knowledge in the area of relationships and nutritional interactions especially the cross-feeding of lactate between bacteria in dark fermentation microbial communities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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“…Dark-fermentation is considered a promising alternative to traditional hydrogen production methods, due to the low estimated production costs [107]. Hydrogen-producing bacteria can be classified as spore-forming obligate anaerobic bacteria, non-sporulating anaerobes and, facultative bacteria (Figure 6) [108]. The Clostridium bacteria, belonging the spore-forming obligate anaerobic microorganisms, are considered the most efficient bacteria in hydrogen production; the fermentation can be both acetatetype (3) and butyrate-type (4) [108].…”

Section: Dark-fermentationmentioning

confidence: 99%

Main Hydrogen Production Processes: An Overview

et al. 2021

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Due to its characteristics, hydrogen is considered the energy carrier of the future. Its use as a fuel generates reduced pollution, as if burned it almost exclusively produces water vapor. Hydrogen can be produced from numerous sources, both of fossil and renewable origin, and with as many production processes, which can use renewable or non-renewable energy sources. To achieve carbon neutrality, the sources must necessarily be renewable, and the production processes themselves must use renewable energy sources. In this review article the main characteristics of the most used hydrogen production methods are summarized, mainly focusing on renewable feedstocks, furthermore a series of relevant articles published in the last year, are reviewed. The production methods are grouped according to the type of energy they use; and at the end of each section the strengths and limitations of the processes are highlighted. The conclusions compare the main characteristics of the production processes studied and contextualize their possible use.

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“…The DF can be conducted at different scales, using different types of bioreactors, such as continuously stirred tank reactors (CSTR), up-flow anaerobic sludge bed (UASB) reactors, anaerobic filter (AF) reactors, and fixed-bed reactors (FBR) [4]; each configuration presents particular features and dynamics, biohydrogen yields, and dominant bacterial species [5]. The DF can also be exploited in cascade reactor arrangements, for example coupling DF to AD in two-stage AD (TSAD) [6].…”

Section: Introductionmentioning

confidence: 99%

Monitoring the Physiological State in the Dark Fermentation of Maize/Grass Silage Using Flow Cytometry and Electrooptic Polarizability Measurements

et al. 2020

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Dark fermentation (DF), a key biohydrogen-producing process, is generally operated as a black-box, by monitoring different operative macroscopic process parameters without evaluating or tracking the physiology of the biotic phase. The biotic phase in DF is constituted by a large variety of microorganisms, mainly fermentative bacteria. The present study uses two (electro)optical techniques, flow cytometry (FC) and frequency-dependent polarizability anisotropy (FDPA) measurements, to gain insights into the physiology of open mixed consortia throughout the DF process. The mixed consortia for DF were obtained from a methanogenic sludge, selecting spore-forming bacteria by means of an acid treatment. Then, DF systems with and without pH control were studied, using as substrate a mixture of maize and grass silage (9:1 w/w). Over the course of fermentation, the butyric pathway was dominant in both systems, and relevant titers of acetate, formate, and ethanol were detected; while hydrogen yields amounted to 20.80 ± 0.05 and 17.08 ± 0.05 NmL/gVS under pH-regulated and non-regulated conditions, respectively. The cytometric pattern analysis of the culture together with microscopic observations made it possible, over the course of fermentation, to identify and track the predominant morphologies in play (i.e., free spore, rod-shaped, and endospore, which are typical of Clostridium spp.). Furthermore, the use of the fluorescent dye DiBAC 4 (3) in FC and FDPA measurements provided similar information regarding the physiological state (PS) of the mixed consortia during the different phases of the culture.

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Stability problems in the hydrogen production by dark fermentation: Possible causes and solutions

Cited by 167 publications

References 169 publications

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Main Hydrogen Production Processes: An Overview

Monitoring the Physiological State in the Dark Fermentation of Maize/Grass Silage Using Flow Cytometry and Electrooptic Polarizability Measurements

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