Stability, sterility, coagulation, and immunologic studies of salmon coagulation proteins with potential use for mammalian wound healing and cell engineering

Laidmäe, Ivo; McCormick, Margaret E.; Herod, Julia; Pastore, Jennifer J.; Salum, Tiit; Sawyer, Evelyn S.; Janmey, Paul A.; Uibo, Raivo

doi:10.1016/j.biomaterials.2006.07.035

Cited by 22 publications

(39 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differences between salmon and mammalian fibrin may be attributed to species-specific variations in the fibrinogen sequence. Fibrinogen is a large glycoprotein containing pairs of three subunit chains Aα, Bβ, and γ. Fibrinogen chains are heavily glycosylated, and differences in carbohydrate modifications between salmon and mammalian fibrinogens may underlie some of the discrepancies between species, including variations in charge and solubility [20,24]. The salmon genome has recently been sequenced and comparison of the predicted amino acid sequences of salmon and mammalian (human and bovine) fibrinogen reveals discrepancies in the location of RGD sequences within the chains.…”

Section: Discussionmentioning

confidence: 99%

“…Salmon fibrin matches the mechanical characteristics of CNS tissue [20,22] and when used to treat rats with dorsal hemisection spinal cord injuries promotes greater locomotor functional recovery, density of serotonergic fibers caudal to the lesion site, and recovery of bladder function than mammalian fibrin [23]. Salmon fibrin has been developed as a human therapeutic and has passed numerous toxicity and immunogenicity tests [24,25]. Although salmon fibrin is an effective scaffold to treat CNS injury [23], it degrades rapidly in vivo (~7 days) and thus is unlikely to provide long-term support for transplanted hNSPCs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering

et al. 2016

View full text Add to dashboard Cite

Human neural stem/progenitor cells (hNSPCs) are good candidates for treating central nervous system (CNS) trauma since they secrete beneficial trophic factors and differentiate into mature CNS cells; however, many cells die after transplantation. This cell death can be ameliorated by inclusion of a biomaterial scaffold, making identification of optimal scaffolds for hNSPCs a critical research focus. We investigated the properties of fibrin-based scaffolds and their effects on hNSPCs and found that fibrin generated from salmon fibrinogen and thrombin stimulates greater hNSPC proliferation than mammalian fibrin. Fibrin scaffolds degrade over the course of a few days in vivo, so we sought to develop a novel scaffold that would retain the beneficial properties of fibrin but degrade more slowly to provide longer support for hNSPCs. We found combination scaffolds of salmon fibrin with interpenetrating networks (IPNs) of hyaluronic acid (HA) with and without laminin polymerize more effectively than fibrin alone and generate compliant hydrogels matching the physical properties of brain tissue. Furthermore, combination scaffolds support hNSPC proliferation and differentiation while significantly attenuating the cell-mediated degradation seen with fibrin alone. HNSPCs express two fibrinogen-binding integrins, αVβ1 and α5β1, and several laminin binding integrins (α7β1, α6β1, α3β1) that can mediate interaction with the scaffold. Lastly, to test the ability of scaffolds to support vascularization, we analyzed human cord blood-derived endothelial cells alone and in co-culture with hNSPCs and found enhanced vessel formation and complexity in co-cultures within combination scaffolds. Overall, combination scaffolds of fibrin, HA, and laminin are excellent biomaterials for hNSPCs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Intraperitoneal injections of salmon fibrin in both rats and rabbits resulted in no negative effects regarding coagulation or cross-reactivity. After repeated administration of salmon fibrin, animals produced antibodies against the salmon proteins, but did not produce antibodies that cross-reacted with host proteins [33, 46]. Also, there was no effect in coagulation tests, a result that was also found in studies of swine that had been treated with salmon fibrin [47, 48].…”

Section: Discussionmentioning

confidence: 99%

“…Compared to mammalian fibrin, salmon fibrin promotes more neurite growth, shows a slower degradation profile, and is non-toxic in animal models [26]. Further, salmon fibrin clots readily at temperatures as low as 0°C, while human fibrinogen activated by thrombin clots slowly below 23°C [33]; this difference provides an advantage in situations where hypothermia must be used. A swine aortotomy model demonstrated salmon fibrin dressing to be effective in controlling severe bleeding [34].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The potential for salmon fibrin and thrombin to mitigate pain subsequent to cervical nerve root injury

et al. 2011

Self Cite

View full text Add to dashboard Cite

Nerve root compression is a common cause of radiculopathy and induces persistent pain. Mammalian fibrin is used clinically as a coagulant but presents a variety of risks. Fish fibrin is a potential biomaterial for neural injury treatment because it promotes neurite outgrowth, is non-toxic, and clots readily at lower temperatures. This study administered salmon fibrin and thrombin following nerve root compression and measured behavioral sensitivity and glial activation in a rat pain model. Fibrin and thrombin each significantly reduced mechanical allodynia compared to injury alone (p<0.02). Painful compression with fibrin exhibited allodynia that was not different from sham for any day using stimulation by a 2 g filament; allodynia was only significantly different (p<0.043) from sham using the 4 g filament on days 1 and 3. By day 5, responses for fibrin treatment decreased to sham levels. Allodynia following compression with thrombin treatment were unchanged from sham at any time point. Macrophage infiltration at the nerve root and spinal microglial activation were only mildly modified by salmon treatments. Spinal astrocytic expression decreased significantly with fibrin (p<0.0001) but was unchanged from injury responses for thrombin treatment. Results suggest that salmon fibrin and thrombin may be suitable biomaterials to mitigate pain.

show abstract

Natural Biomaterials from Biodiversity for Healthcare Applications

Insuasti-Cruz

Suárez-Jaramillo

Mena

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

Natural biomaterials originating during the growth cycles of all living organisms have been used for many applications. They span from bioinert to bioactive materials including bioinspired ones. As they exhibit an increasing degree of sophistication, natural biomaterials have proven suitable to address the needs of the healthcare sector. Here the different natural healthcare biomaterials, their biodiversity sources, properties, and promising healthcare applications are reviewed. The variability of their properties as a result of considered species and their habitat is also discussed. Finally, some limitations of natural biomaterials are discussed and possible future developments are provided as more natural biomaterials are yet to be discovered and studied.

show abstract

Stability, sterility, coagulation, and immunologic studies of salmon coagulation proteins with potential use for mammalian wound healing and cell engineering

Cited by 22 publications

References 22 publications

Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering

Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering

The potential for salmon fibrin and thrombin to mitigate pain subsequent to cervical nerve root injury

Natural Biomaterials from Biodiversity for Healthcare Applications

Contact Info

Product

Resources

About