Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions

Çuhadar, Serap; Atay, Ayşenur; Köseoğlu, Mehmet; Dirican, Ahmet; Hür, Aysel

doi:10.11613/bm.2012.023

Cited by 73 publications

(64 citation statements)

References 15 publications

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“…This might be due to a range of studies applied different test for comparing sample stability study e.g. repeated measure ANOVA, 14 One way ANOVA, 15 Friedman test 16 and paired sample Student's ttest. As we want to find out difference between 0.5 hours to 24 hours and 0.5 hour to 48 hours sample we had applied paired sample Student's ttest also verified with repeated measure ANNOVA.…”

Section: Discussionmentioning

confidence: 99%

Study of specimen stability for biochemical parameters

2020

IJCBR

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Introduction: The blood sample is collected and transported to the laboratory for analysis, during this time the samples bares many extra-analytical factors prior to analysis. Contact time of plasma or serum with the blood cells is one of these factors because of ongoing cell metabolism as well as active and passive movement of analytes in cellular compartments. Aim and Objective: The aim of our study is to determine the stability of different seventeen biochemical parameters in primary collection (without separation of serum/plasma) and Secondary collection (with separation of serum/plasma) tube. Materials and Methods: Hundred patients' venous blood sample were collected in plain vacutainer and fluoride vacutainer (primary tube), set of the seventeen biochemical analytes like sodium, potassium, ionized calcium, chloride, glucose, creatinine, urea, uric acid, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, total protein, albumin, bilirubin, triglyceride, total cholesterol and HDL-cholesterol tests were analysed. Once the tests were carried out from primary tube, approximately half serum was separated into two aliquots (secondary collection tube). All tubes were stored at 2 to 8° C. After 24 hr and 48 hr again same sate of tests were performed on the same equipment. Statistical analysis was done after that. Results: Out of seventeen analytes most analytes remained stable in both primary and secondary tubes up to 48 hr at 2 to 8° C, these includes chloride, creatinine, urea, uric acid, total bilirubin, total protein, albumin. alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Plasma glucose decreased in both primary and secondary collection tube but concentration is within significant change limit (SCL). While serum potassium is increase 28.84% at 24 hr and 75.58% at 48 hr; concentration is also out of SCL range in primary collection tube even though samples stored at 2 -8⁰ C. Conclusion: Most biochemical analytes will remain stable for 48 hr at 2 -8⁰ C, only few analytes like plasma glucose and serum potassium requires stringent control before serum-clot separation.

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Section: Discussionmentioning

confidence: 99%

Study of specimen stability for biochemical parameters

2020

IJCBR

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“…(35). In their study the authors have assessed the stability of some most common biochemical parameters in serum separator tubes with or without gel barrier subjected to various storage conditions.…”

Section: Preanalytical Phase Studies Published In This Issue Of Biochmentioning

confidence: 99%

Preanalytical phase – a continuous challenge for laboratory professionals

Šimundić¹,

Lippi

2012

Biochem Med

118

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Preanalytical phase is the most vulnerable part of the total testing process and is considered to be among the greatest challenges to the laboratory professionals. However, preanalytical activities, management of unsuitable specimens and reporting policies are not fully standardized, nor harmonized worldwide. Several standards related to blood sampling and sample transportation and handling are available, but compliance to those guidelines is low, especially outside the laboratory and if blood sampling is done without the direct supervision of the laboratory staff. Furthermore, for some most critical procedures within the preanalytical phase, internationally accepted guidelines and recommendations as well as related quality measures are unfortunately unavailable. There is large heterogeneity in the criteria for sample rejection, the diff erent strategies by which unacceptable samples are managed, processed and test results reported worldwide. Management of unacceptable specimens warrants therefore immediate harmonization. Alongside the challenging and long road of patient safety, preanalytical phase off ers room for improvement, and Editors at Biochemia Medica Journal definitely hope to continue providing a respective mean for reporting studies on diff erent preanalytical phase topics. With pleasure and delight we invite potential future authors to submit their articles examining the quality of various preanalytical activities to Biochemia Medica. We will keep nurturing this topic as our prominent feature and by this we hope to be able to deliver valid evidence for some future guidelines and recommendations.

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“…The rate of decrease in plain tubes is sensitive to the clot contact time and temperature. Because glycolysis occurs in the cellular part of the blood, complete barrier formation of the gel separator tube and storage in the refrigerator protects glucose concentrations (−2.1%, clinically insignificant) for up to 36 h of storage 33 . To ease the effect of glycolysis, NaF addition into the tube for its inhibitory effect on enolase, an enzyme in the glycolytic pathway, has been recommended, however, during the first 1-2 h the effect was found as inadequate 34 .…”

Section: Glucosementioning

confidence: 99%

Preanalytical variables and factors that interfere with the biochemical parameters: A review

uhadar¹

2013

OA Biotechnology

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IntroductionThe total testing process, which begins with the order of the physician, includes the preanalytical, analytical and postanalytical phases and ends with the results ready for interpretation. To obtain a reliable test result requires detection of all steps however, to control the preanalytical stage is such a complex way that much of the steps are human dependent and out of the laboratory's control thus occupies the most erroneous part of the total testing. The worst side is that the error in that stage often becomes apparent in the analytical or postanalytical phase.The aim is to highlight the multifactorial human dependent erroneous stage that affects the biochemical routine test results, which can be easily prevented with awareness and laboratory staff education. We will concentrate on the preanalytical errors which include specimen type selection, blood collection, blood collection equipments and the factors interfering with biochemical tests. ConclusionThe quality of the analytical phase increased due to the interest, tight control and following the quality procedures both internal and external established by the medical laboratories and manufacturers.

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Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions

Cited by 73 publications

References 15 publications

Study of specimen stability for biochemical parameters

Study of specimen stability for biochemical parameters

Preanalytical phase – a continuous challenge for laboratory professionals

Preanalytical variables and factors that interfere with the biochemical parameters: A review

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