Stabilization and particle morphology of prune dwarf virus

Halk, Edward L.; Fulton, Robert W.

doi:10.1016/0042-6822(78)90389-6

Cited by 24 publications

(12 citation statements)

References 25 publications

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“…The segments of the genome are encapsidated by a single coat protein (CP) into three isometric particles with a diameter of 19–26 nm. The virus particles contain 14% nucleic acid and 86% protein and the CP is about 24 kDa (Brunt et al, 1996; Halk and Fulton, 1978). …”

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Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

Öztürk

Çevik

2015

The Plant Pathology Journal

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Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84–99% to 81–100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

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Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

Öztürk

Çevik

2015

The Plant Pathology Journal

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“…National Pickling) cotyledons following essentially the method reported by Halk & Fulton (1978) for the purification of prune dwarf virus. First-strand cDNA was synthesized from unfractionated virion RNA by priming with random hexamers.…”

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Nucleotide sequence of apple mosaic ilarvirus RNA 4

Sánchez‐Navarro

Pallás

1994

Journal of General Virology

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The complete nucleotide sequence of apple mosaic ilarvirus RNA 4 was obtained from cloned cDNAs and direct sequencing of the 5'-terminal RNA region. The sequence is 891 nucleotides long and can encode a protein of 226 amino acids (M r 25171) that, by analogy to alfalfa mosaic virus (A1MV) and tobacco streak virus (TSV), should correspond to the coat protein (CP). Database comparisons showed that no significant similarity to other proteins was apparent. Analysis of the CP sequence revealed a putative 'zinc finger' domain and a region rich in basic residues at the amino-terminal portion of the protein, similar to that of TSV. The secondary structure proposed for the 3'-terminal region ofRNA 4 shows the presence of three hairpin structures flanked by the tetranucleotide AUGC that are highly similar to those previously described in the RNA 4 species from A1MV and TSV. These results support the idea that both features (metal-binding domain and highly conserved hairpin structures) are characteristics of ilarviruses and are probably involved in the peculiar 'genome activation' phenomenon described in these viruses.

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“…Oval or bacilliform particles have been found for members and possible members of the ilarvirus group, i.e. alfalfa mosaic (Gibbs et al, 1963), black raspberry latent (Converse and Lister, 1969), citrus leaf rugose and citrus variegation (Gonsalves and Garnsey, 1976), elm mottle (Jones and Mayo, 1973), lilac ring mottle (Van der Meet et al, 1976), prune dwarf (Halk and Fulton, 1978), Prunus necrotic ringspot (Lister and Saksena, 1976), rose mosaic (Basit and Francki, 1972), and Tulare apple mosaic (Fulton, 1967). In our tests the SpLV did not react with antisera to the ilarviruses of apple mosaic, elm mottle, lilac ring mottle, Prunus necrotic ringspot and tobacco streak, but ilarviruses in general are poorly immunogenic and there are only some serological relationships between members within subgroups.…”

Section: Discussionmentioning

confidence: 98%

Spinach latent virus, a new ilarvirus seed-borne in Spinacia oleracea

Bos¹,

Huttinga²,

Maat³

1980

Netherlands Journal of Plant Pathology

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During 1977 and 1978 an apparently new virus was isolated from samples of 12 out of 142 lots of spinach seed from a number of countries which did not produce symptoms in spinach. In one sample seed infection was over 50~. The virus was later found to be identical with a virus encoded GE36, earlier presumed to have been isolated from apple and pear (Van der Meer, 1968;Maat and Vink, 1971). It has now been further characterized and named spinach latent virus.Seventeen out of 36 plant species tested were susceptible, most without producing symptoms.Myzuspersicae did not transmit the virus, but rates of seed transmission in artificial hosts were high: 53 ~ in Celosia cristata, over 90~ in Chenopodium quinoa, 30~ in Nicotiana rustica, 72~ in N. tabaeum 'Samsun', 90% in 'White Burley' and 94~ in 'Xanthi'. Infection is in the embryo.The virus can also be detected in dry seeds. Purified virus contained three components sedimenting at 87, 98 and 108 S. In the electron microscope the virus particles were irregularly spherical and c. 27 nm in diameter. They were hard to detect in crude plant sap. Some particles were bacilliform. The buoyant density in cesium sulphate was 1.269 x 103 kg.m-a. Five RNA components were detected having relative molecular masses of 1.30, 1.18, 0.91, 0.35 and 0.27 x 106, respectively. To induce infection the three largest components are required plus the two smallest components or the coat protein. Molecular mass of the coat protein subunit was 28000. These characters are typical of ilarviruses.The virus proved weakly immunogenic. An antiserum with a titre of 64 was produced. No serological differences could be found between the type isolate from spinach and GE36. The virus did not react with antisera to any of 36 spherical viruses and alfalfa mosaic virus.Additional keyword: GE36 virus.

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Stabilization and particle morphology of prune dwarf virus

Cited by 24 publications

References 25 publications

Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

Genetic Diversity in the Coat Protein Genes of Prune dwarf virus Isolates from Sweet Cherry Growing in Turkey

Nucleotide sequence of apple mosaic ilarvirus RNA 4

Spinach latent virus, a new ilarvirus seed-borne in Spinacia oleracea

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