This paper investigates the Luenberger observer design problem for non-autonomous control semilinear evolution equations with disturbances in Banach spaces. Then, the practicalstabilization problem of the system is solved, yielding a compensator based on the Luenberger observer by using integral inequalities of the Gronwall type. Sufficient conditions of the controller and observer problem are satisfied, we show that the proposed controller with estimatedstate feedback from the proposed practical Luenberger observer will achieve global practical stabilization. We develop novel ideas and techniques, which present the further development of mathematical control theory. Furthermore, an example is given to show the applicability of our theoretical results.