Stabilization of enzyme immunoassays for atrazine

Dankwardt, Andrea; Müller, Jutta; Hock, Bertold

doi:10.1016/s0003-2670(97)00607-7

Cited by 22 publications

(11 citation statements)

References 30 publications

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“…Polychlorinated biphenyls (PCBs) are found throughout the food chain (fish extracts, clams, milk) and Bender and Sadik [148] produced an electrochemical immunosensor to detect A2.4.5 Modes of Analysis Using Biosensors 271 Artificial sweeteners [131] Alcohol dehydrogenase (ADH), glycerokinase (GK), glycerol-3-phosphate oxidase (GPO), phospholipase D (PL), choline oxidase (COx), a-chymotrypsin (CT) them. Dankwardt and Hock [149] reviewed the use of immunoassays to detect pesticides in foods and described the use of immunosensors. Marine toxins have become of increasing interest in recent years, as the incidence of outbreaks has been rising.…”

Section: Immunoassaysmentioning

confidence: 99%

Sensors and Food Quality

Connell

Guilbault

2001

Sensors Update

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Production processes require quality control. The quality of food going to the consumer must always be assured. Traditionally this was performed by taking a sample and sending it to a remote laboratory. While this is successful for most samples, this was not always the case. Having remote laboratories means that there could be a significant lag-time between sampling and results. Some unstable samples may have changed or the source material could have been already used/processed by the time they are analyzed. Sensor-based systems, especially those incorporated within flow injection analyzers, can overcome this problem. As the systems can be small and inexpensive relative to other automated analyzers, they can easily be fitted onsite. With the sensors fitted within the production process, a nearly instant reading can be achieved. The advantages and disadvantages of physical, chemical, and biosensors and their applicability to food analysis are discussed. Particular attention is focused on the improvements made to the underlying technology used in biosensor construction.

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Section: Immunoassaysmentioning

confidence: 99%

Sensors and Food Quality

Connell

Guilbault

2001

Sensors Update

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“…Particular attention has been paid to immunochemical methods reported for those EDCs rated as high to medium risk of exposure for the human and wildlife population, as occurs with APEs, PE, BPA, PCBs, PCDDs and PCDFs. We will not go through the immunochemical determination of pesticides with suspicious ED activity, since the reader may find other reviews dealing with this topic [21,24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

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This paper is a review with more than 100 references discussing the immunochemical methods reported in the literature for the most important man-made chemicals with suspected endocrine disrupting activity. Details regarding immunizing hapten design, antibody production, and the features (limit of detection, dynamic range, specificity) of the most important immunochemical methods developed (ELISA, FIIA, immunosorbents, immunosensors, etc.) are presented for important environmental pollutants such as bisphenol A, phthalates, alkylphenol polyethoxylates, alkylphenols, polychlorinated biphenyl compounds, and dioxins. Availability of commercial reagents and methods is reported.

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“…Therefore, some additives were tested for their stabilizing effect and interference on antigen immobilized and antigen‐antibody interaction in the microwell plates. In order to protect of immobilized antigen, sugars (sucrose), polymers (PVP, PVA, PEG, etc), gelatin, or BSA were added during the drying process and storage (Dankwardt, Müller, & Hock, ).…”

Section: Resultsmentioning

confidence: 99%

Development and optimization of an immunoassay for the detection of Hg(II) in lake water

Jin

et al. 2019

Food Science & Nutrition

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In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH 3 Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.

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Stabilization of enzyme immunoassays for atrazine

Cited by 22 publications

References 30 publications

Sensors and Food Quality

Sensors and Food Quality

Immunochemical determination of xenobiotics with endocrine disrupting effects

Development and optimization of an immunoassay for the detection of Hg(II) in lake water

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