Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Abstract

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5-50 µg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 µM ONOO -or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

Elucidation of Structures of New Ellagitannins From Plants of Euphorbiaceous

“…Recently, researches carried out by scientists of Bioorganic Chemistry of the Academy of Sciences of Uzbekistan, have shown prosperity of use of natural phenolic compounds as an antiviral, antioxidant, anti-tumor agents [14][15][16][17][18][19][20]. It was found that these compounds have high biological activity, which manifests itself in suppressing the activity of various viral infections, redox enzymes and etc.…”

Effects of hydrolysable tannins on native and artificial biological membranes

“…Alteration in membrane con ductance caused by tannin application can be one of the factors increasing the leakage current. In earlier studies of the effects of this tannin type on erythrocyte ghosts, relative hydrophobicity of tannin was reported [33]. Here we study effects of Rutan and its hydrolys able tannin on artificial membranes.…”

Study of acute genotoxic potential of an aqueous extract of Schinus terebinthifolius Raddi: an in vivo micronucleus assay

“…There is speculation that this effect may be related to the presence of tannins in the Schinus terebinthifolius Raddi [48][49]. According to Olchowik and colleagues (2012) [50], the tannins are classified into two main groups, which structures are very different, although all of them have polyhydroxy phenols molecules or their derivatives. First group consists in hydrolysable tannins (such as tannic acid), commonly used for tanning leather and the second one contains other types of tannins, found in greater quantity and of greater importance in food, being called condensed tannins, which basic structure are related to the structure of catechin and 3', 4', 5, 7 -hydroxy-flavonoid [50].…”