Stabilization of gelatin films by crosslinking with genipin

Bigi, Adriana; Cojazzi, Gianna; Panzavolta, Silvia; Roveri, Norberto; Rubini, Katia

doi:10.1016/s0142-9612(02)00235-1

Cited by 590 publications

(518 citation statements)

References 31 publications

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“…1) is a chemical crosslinker, with very low toxicity, obtained from the natural occurring product geniposide that is extracted from Gardenia jasminoides fruits. Genipin has attracted great attention due to its ability to crosslink polymer chains containing primary amines, namely proteins such as gelatin [10][11][12] and polysaccharides such as chitosan [10,13]. The cytotoxicity of genipin is ca.…”

Section: Introductionmentioning

confidence: 99%

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

2013

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Gold gelatin hydrogel nanocomposites crosslinked with genipin have been prepared, and the effect of citrate capped Au nanoparticles (NPs) as nanofillers in the crosslinking and swelling of gelatin and release of a model drug (methylene blue) from gelatin nanocomposites have been investigated. The citrate-capped Au NPs prevented the crosslinking reaction between the gelatin and genipin and resulted in less crosslinked hydrogels. Although less crosslinked, the Au gelatin nanocomposites swelled less than the unfilled crosslinked gelatin. The gelatin composites were optically active and thermo-sensitive in a temperature range acceptable for living cells. In vitro release studies demonstrated that the irradiation of the composite gels with monochromatic green light (10532 nm, 100 mW) increases the release of the encapsulated methylene blue, most likely due to the photothermal effect of Au nanoparticles. This opens the possibility to explore the application of these nanocomposites as carriers in remotely controlled light-triggered drug release.

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Section: Introductionmentioning

confidence: 99%

Effects of Au nanoparticles on thermoresponsive genipin-crosslinked gelatin hydrogels

2013

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“…To further reduce the cytotoxicity, a more benign cross-linking method could be used such as photo-crosslinker, genipin or natural phenolic compounds. [19][20][21] To form square arrayed holes throughout nanofiber membranes, we first immersed membranes into liquid nitrogen to make the materials brittle (below the glass transition temperature) and then created holes with a punch. Following our established protocols, we expanded nanofiber membranes with square arrayed holes in a mold generated by 3D printer using a modified gas foaming technique (Fig.…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional nanofiber scaffolds with arrayed holes for engineering skin tissue constructs

Xie

Carlson

et al. 2017

MRS Communications

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Three-dimensional (3D) scaffolds composed of poly(ε-caprolactone) and gelatin nanofibers were fabricated by a combination of electrospinning and modified gas-foaming. Arrayed holes throughout the scaffold were created using a punch under cryo conditions. The crosslinking with glutaraldehyde vapor improved the water stability of the scaffolds. Cell spheroids of green fluorescent protein-labeled human dermal fibroblasts were prepared and seeded into the holes. It was found that the fibroblasts adhered well on the surface of nanofibers and migrated into the scaffolds due to the porous structures. The 3D nanofiber scaffolds may hold great potential for engineering tissue constructs for various applications.

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“…Natural crosslinkers, such as genipin, have already shown improved biocompatibility over glutaraldehyde in tissue fixation [135][136][137]. Other groups have demonstrated incorporation of genipin into natural ECM hydrogels [73,138] and natural electrospun nanofibers [53].…”

Section: Future Perspectivementioning

confidence: 99%

“…Laminin Peripheral nerves [43] Genipin Skin Nerves Cartilage [53,136] [73] [135] Silk/fibroin Skin Ligament Mineralized osteodentin [53] [54] [92] Chitin/chitosan Cardiac muscle Bone [66] [67]…”

Section: Introductionmentioning

confidence: 99%