Stabilization of Glucose in Blood Specimens: Mechanism of Delay in Fluoride Inhibition of Glycolysis

Mikesh, Leann M.; Bruns, David E.

doi:10.1373/clinchem.2007.102160

Cited by 94 publications

(60 citation statements)

References 5 publications

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“…While laboratories have improved the measurement procedures for plasma glucose, loss of glucose from blood samples before analysis remains a threat to accuracy of results. The traditional use of sodium fluoride (NaF) alone is unsatisfactory as it does not begin to inhibit glycolysis until 90-120 min have elapsed after blood is collected [2,3]. The failure of NaF to properly control glycolysis leads to falsely low plasma glucose concentrations and, potentially, failure to detect diabetes.…”

mentioning

confidence: 99%

Stabilization of glucose in blood samples: out with the old, in with the new

Gambino

Bruns

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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confidence: 99%

Stabilization of glucose in blood samples: out with the old, in with the new

Gambino

Bruns

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Glucolysis is known to cause falling glucose concentrations in blood samples after collection, and antiglycolytic agents can be added in order to maintain glucose levels [19,20,21]. Such antiglycolytic agents can interfere with cellular functions, and are therefore not used during studies of activated blood cells.…”

Section: Discussionmentioning

confidence: 99%

“…Wilcoxon signed rank test revealed that endogenous insulin concentration after incubation at 37 ºC for 6 hours was significantly reduced from baseline 48 (36-94) to 23 (18)(19)(20)(21)(22)(23)(24)(25)(26)(27) pmol/L (p=0.03) (Figure 2). …”

Section: Insulin Concentrationsmentioning

confidence: 98%

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Reduction of glucose and insulin concentrations during in vitro incubation of human whole blood at different temperatures

Beitland

Opdahl²,

Aspelin³

et al. 2013

J Diab Res Clin Met

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Background: Incubation of human whole blood at body temperature has been used in numerous studies without addition of glucose and insulin. The purpose of the study was to examine glucose concentration changes during in vitro incubation of human whole blood at different temperatures, and whether it was affected by addition of insulin and bacterial endotoxin. We also wanted to quantify changes in endogenous insulin concentrations during incubation for six hours at 37 ºC. Methods: Young, healthy and fasting males donated whole blood. Glucose concentrations were compared at baseline and after six hours incubation at 37 ºC, 22 ºC or 0 ºC, in aliquots with and without addition of insulin and bacterial endotoxin. Glucose data are presented as mean (± 1 standard deviation). Endogenous insulin concentrations in aliquots without addition were measured at baseline and after six hours incubation at 37 ºC, data are shown as median (interquartile range). Results: Glucose concentration at baseline was 5.3 (± 0.6) mmol/L. After incubation for six hours at different temperatures, the glucose level at 37 ºC was 1.0 (± 0.5) mmol/L (p<0.01), at 22 ºC 3.0 (± 0.6) mmol/L (p<0.01), and at 0 ºC 5.4 (± 0.7) mmol/L (p=0.95). The decline in glucose concentration seemed to be independent of addition of insulin and bacterial endotoxin. Endogenous insulin levels decreased from baseline 48 (36-94) pmol/L to 23 (18-27) pmol/L (p=0.03) during six hours incubation at 37 ºC. Conclusions: Glucose concentration was markedly reduced during in vitro incubation of whole blood from healthy volunteers for six hours at 37 ºC and 22 ºC, but was maintained at 0 ºC. Endogenous insulin level after six hours incubation of whole blood at 37 ºC was more than halved compared to baseline. During in vitro studies of glucose and/or insulin effects lasting for hours, measures must be undertaken to maintain stable glucose and/or insulin concentrations.

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“…•송정한 4 •윤여민 5 •박해일 6 •성문우 7 •황동희 8 •김현수 9 •김정호 10 •이봉숙 11 •황유성 12 Background : Accurate measurement of blood glucose concentrations is essential for defining diabetes, and the minimization of ex vivo glycolysis has been recommended. Recent guidelines advocate two kinds of methods for sample collection and processing: either the sodium fluoride (NaF) method or immediate refrigeration using a serum separation tube (SST).…”

mentioning

confidence: 99%