Stabilization of Oxidative Stress 1 Year after Kidney Transplantation: Effect of Calcineurin Immunosuppressives

Vostalová, Jitka; Galandáková, Adéla; Svobodová, Alena Rajnochová; Kajabová, Markéta; Schneiderka, Petr; Zapletalová, Jana; Štrebl, Pavel; Zadrazil, J

doi:10.3109/0886022x.2012.699874

Cited by 13 publications

(12 citation statements)

References 32 publications

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“…To determine the effect of immunosuppression choices in this drastic reduction of oxidative stress after kidney transplant, Vostálová et al [30] followed 70 patients after kidney transplant. The patients were divided into two groups: those receiving cyclosporine and those receiving tacrolimus.…”

Section: Kidney Transplant and Oxidative Stressmentioning

confidence: 99%

Chronic kidney disease, kidney transplantation and oxidative stress: a new look to successful kidney transplantation

Tabriziani

Lipkowitz

Vuong

2017

Clinical Kidney Journal

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Oxidative stress plays a key role in the pathophysiological process of uremia and its complications, particularly in cardiovascular disease. The level of oxidative stress markers is known to increase as chronic kidney disease progresses and correlates significantly with the level of renal function. Hemodialysis and peritoneal dialysis are major modes of renal replacement therapy for end-stage renal disease patients, but unfortunately they are also accompanied by increased oxidative stress. Successful kidney transplantation, however, results in near normalization of the antioxidant status and lipid metabolism by eliminating free radicals despite the surge of oxidative stress caused by the surgical procedure and ischemic injury to the organ during the operation. This success is associated with both improved renal function, reduced cardiovascular complications and overall improved morbidity and mortality. Measuring oxidative stress markers such as malondialdehyde is promising in predicting allograft survival and delayed graft function.

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Section: Kidney Transplant and Oxidative Stressmentioning

confidence: 99%

Chronic kidney disease, kidney transplantation and oxidative stress: a new look to successful kidney transplantation

Tabriziani

Lipkowitz

Vuong

2017

Clinical Kidney Journal

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“…Studies have demonstrated that oxidative stress is also an important intracellular messenger, activating various intracellular signaling pathways (16,17) and mediating cell stress responses and injury responses. Oxidative stress is an important pathogenic factor influencing the recovery of short-and long-term function following renal transplantation (18)(19)(20). The progression of CAN may be alleviated according to the characteristics of its different stages and the application of suitable antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of curcumin against cyclosporine A‑induced rat nephrotoxicity

et al. 2018

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This study explored the potential value of curcumin, a natural product, in the protection of CsA‑induced nephrotoxicity. The aim of the present study was to investigate the effects of curcumin on Cyclosporine A (CsA)‑induced renal oxidative stress and determine the potential underlying molecular mechanisms of the renal protective effects of Cur. HK‑2 human renal cells were co‑treated with CsA and various doses of Cur. Cell survival rate was determined by an MTT assay, total cellular protein was collected and oxidative stress in cell homogenates was evaluated by determining the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px) and catalase (CAT), the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), and total antioxidant capacity. Furthermore, Bcl‑2 and Bcl‑2‑associated X (Bax) protein expression was measured by western blot analysis. In addition, a CsA‑induced nephrotoxicity (CAN) rat model was also established. Renal function was analyzed by measuring creatinine (Crea) and blood urea nitrogen (BUN) in the serum of rats, and histopathological examination was performed on renal tissues using hematoxylin and eosin staining, periodic acid‑Schiff staining and nuclear factor‑κB (NF‑κB) immunostaining. The results demonstrated that treatment of HK‑2 cells with CsA significantly increased ROS and MDA levels, and decreased the activities of SOD, GSH‑Px and CAT, compared with the control group. However, these effects of CsA were dose‑dependently improved by treatment with Cur. In addition, Cur treatment increased Bcl‑2 and decreased Bax protein in HK‑2 cells, compared with cells treated with CsA alone. In the CAN rat model CsA (30 mg/kg) treatment significantly elevated serum Crea levels and BUN, but lowered endogenous Crea clearance rate, compared with the control group. Co‑administration of Cur with CsA significantly reversed the effects of CsA on serum Crea levels, BUN and Crea clearance rate (Ccr). Additionally, Cur alleviated CsA‑induced renal cell injury, as less vacuolar degeneration of glomerular cells was observed compared with the CsA alone group. In conclusion, Cur may increase renal antioxidant capacity and reduce the Bax/Bcl‑2 ratio, subsequently improving CsA‑induced renal failure and renal tubular deformation and cell vacuolization.

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“…Improved renal function after kidney transplant may be associated with decreased oxidative stress, but the relation between immunosuppressive therapy and decreased oxidative stress is unclear. 20 The different results of these studies may be attributed to different biomarkers for oxidative stress. The evaluation of biomolecules that are modified by glutathione oxidation, such as lipid peroxidation products (thiobarbituric acid reactive substances) or advanced oxidation protein products, may be more sensitive than the evaluation of antioxidant enzyme activity or total antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

“…The evaluation of biomolecules that are modified by glutathione oxidation, such as lipid peroxidation products (thiobarbituric acid reactive substances) or advanced oxidation protein products, may be more sensitive than the evaluation of antioxidant enzyme activity or total antioxidant capacity. 20 The enzyme PON is sensitive to oxidants and is inactivated by lipid peroxides. Therefore, increased oxidative stress may decrease PON activity and impair the antioxidant activities of HDL in dialysis patients.…”

Section: Discussionmentioning

confidence: 99%

“…5,[13][14][15] However, there are few studies that compare the effect of calcineurin inhibitors on oxidant and an antioxidant system indices in kidney transplant recipients. [16][17][18][19][20] The purpose of this prospective study was to evaluate the effects of the calcineurin inhibitors tacrolimus and cyclosporine on cardiovascular disease risk factors, including high-sensitivity CRP (hsCRP), homocysteine, malondialdehyde (MDA, a lipid peroxidation biomarker of oxidative stress), and activity of PON and arylesterase (components of the antioxidant system), in kidney transplant recipients.…”

mentioning

confidence: 99%

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Kahvecioğlu

Güllülü²,

Dirican³

2014

Exp Clin Transplant

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Objectives: Cardiovascular disease is a common cause of morbidity and mortality in patients with chronic kidney failure, before and after a kidney transplant. Oxidation of lipoproteins that contain apolipoprotein B may contribute to the initiation of atherosclerosis. Paraoxonase may prevent cardiovascular disease. We compared the effects of different calcineurin inhibitors on risk factors for cardiovascular disease in kidney transplant recipients. Materials and Methods: In 16 kidney transplant recipients, treatment included tacrolimus in 8 patients and cyclosporine in 8 patients. Hemoglobin, glucose, renal function, lipid parameters, high-sensitivity C-reactive protein, homocysteine, malondialdehyde, paraoxonase activity, and arylesterase activity were measured before transplant and at 1, 6, and 12 months after the transplant. Results: The levels of homocysteine and malondialdehyde did not change significantly in patients who received either tacrolimus or cyclosporine. The high-sensitivity C-reactive protein was decreased (tacrolimus group, 1 mo) and increased (cyclosporine group, 6 and 12 mo) after the kidney transplant. Paraoxonase activity was increased (tacrolimus group, 1 mo). Arylesterase activity was increased (tacrolimus group, 1, 6, and 12 mo; cyclosporine group, 1 and 6 mo). The percentage of change in arylesterase activity was higher at 12 months in the tacrolimus than in the cyclosporine group. Conclusions: Tacrolimus may be more effective than cyclosporine in improving risk factors for cardiovascular disease after kidney transplant.

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Stabilization of Oxidative Stress 1 Year after Kidney Transplantation: Effect of Calcineurin Immunosuppressives

Cited by 13 publications

References 32 publications

Chronic kidney disease, kidney transplantation and oxidative stress: a new look to successful kidney transplantation

Chronic kidney disease, kidney transplantation and oxidative stress: a new look to successful kidney transplantation

Protective effect of curcumin against cyclosporine A‑induced rat nephrotoxicity

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