Stabilization of Pores in Lipid Bilayers by Anisotropic Inclusions

Fošnarič, Miha; Kralj‐Iglič, Veronika; Bohinc, Klemen; Iglič, A.; May, Sylvio

doi:10.1021/jp035035a

Cited by 73 publications

(70 citation statements)

References 58 publications

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“…However, it cannot capture all details of complex membrane systems. For instance, IMM1-torus does not account for membrane deformations and lipid repacking (which, in principle, might be included using elasticity theory [55, 56] or mean field theory [57]) and it does not allow the shape of pore to change. Therefore, it cannot be used to study events that lead toward pore formation (such as, membrane thinning, expansion of the lipid headgroups and bending of the bilayer) or pore formation per se.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptides bind more strongly to membrane pores

Mihajlovic¹,

Lazaridis²

2010

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Antimicrobial peptides (AMPs) are small, usually cationic peptides, which permeabilize bacterial membranes. Understanding their mechanism of action might help design better antibiotics. Using an implicit membrane model, modified to include pores of different shapes, we show that four AMPs (alamethicin, melittin, a magainin analogue, MG-H2, and piscidin 1) bind more strongly to membrane pores, consistent with the idea that they stabilize them. The effective energy of alamethicin in cylindrical pores is similar to that in toroidal pores, whereas the effective energy of the other three peptides is lower in toroidal pores. Only alamethicin intercalates into the membrane core; MG-H2, melittin and piscidin are located exclusively at the hydrophobic/hydrophilic interface. In toroidal pores, the latter three peptides often bind at the edge of the pore, and are in an oblique orientation. The calculated binding energies of the peptides are correlated with their hemolytic activities. We hypothesize that one distinguishing feature of AMPs may be the fact that they are imperfectly amphipathic which allows them to bind more strongly to toroidal pores. An initial test on a melittin-based mutant seems to support this hypothesis.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial peptides bind more strongly to membrane pores

Mihajlovic¹,

Lazaridis²

2010

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…55,56 While it was previously acknowledged that membrane composition and shape are interdependent, 34,57−59 the orientational ordering model provides a unified explanation of the above feature, and has been reviewed extensively elsewhere. 37,45,60,61 Figure 5 presents some of these nanostructures, with buds and nanovesicles of the erythrocyte membrane and nanotubules observed in urothelial cancer cells.…”

Section: Deviatoric Elasticity May Stabilize Anisotropic Nanostructuresmentioning

confidence: 99%

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Kralj‐Iglič¹

2012

IJN

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Abstract:Membranous nanostructures, such as nanovesicles and nanotubules, are an important pool of biological membranes. Recent results indicate that they constitute cell-cell communication systems and that cancer development is influenced by these systems. Nanovesicles that are pinched off from cancer cells can move within the circulation and interact with distant cells. It has been suggested and indicated by experimental evidence that nanovesicles can induce metastases from the primary tumor in this way. Therefore, it is of importance to understand better the mechanisms of membrane budding and vesiculation. Here, a theoretical description is presented concerning consistently related lateral membrane composition, orientational ordering of membrane constituents, and a stable shape of nanovesicles and nanotubules. It is shown that the character of stable nanostructures reflects the composition of the membrane and the intrinsic shape of its constituents. An extension of the fluid mosaic model of biological membranes is suggested by taking into account curvature-mediated orientational ordering of the membrane constituents on strongly anisotropically curved regions. Based on experimental data for artificial membranes, a possible antimetastatic effect of plasma constituents via mediation of attractive interaction between membranous structures is suggested. This mediated attractive interaction hypothetically suppresses nanovesiculation by causing adhesion of buds to the mother membrane and preventing them from being pinched off from the membrane.

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“…Incorporation of nonphospholipid substances into planar lipid bilayers changes U br as well. The effect is a consequence of the surfactant molecular shape acting to change the spontaneous curvature of the membrane, which is especially important during the defects formation process [12], [49], [50].…”

Section: Discussionmentioning

confidence: 99%

“…The exact molecular mechanisms of the phenomenon are still not fully elucidated; however, it is believed that "pores" in the cell membrane are mostly formed by the rearrangement of lipid molecules. Therefore, artificial lipid bilayer structures in the form of planar lipid bilayers or lipid bilayer vesicles can be used as a model for experimental [10], [11] and theoretical studies of electroporation [12].…”

mentioning

confidence: 99%

A System for the Determination of Planar Lipid Bilayer Breakdown Voltage and Its Applications

Kramar

Miklavčič

Lebar

2009

IEEE Trans.on Nanobioscience

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Abstract-In this paper, we focus on measurement principles used in electroporation studies on planar lipid bilayers. In particular, we point out the voltage-clamp measurement principle that has great importance when the breakdown voltage of a planar lipid bilayer is under consideration; however, it is also appropriate for the determination of other planar lipid bilayer electrical properties such as resistance and capacitance. A new experimental system that is based on the voltage-clamp measurement principle is described. With the use of a generator that can generate arbitrary-type signals, many specific shapes of a voltage signal could be generated, and therefore, the experimental system is appropriate for a broad spectrum of measurements.

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Stabilization of Pores in Lipid Bilayers by Anisotropic Inclusions

Cited by 73 publications

References 58 publications

Antimicrobial peptides bind more strongly to membrane pores

Antimicrobial peptides bind more strongly to membrane pores

Stability of membranous nanostructures: a possible key mechanism in cancer progression

A System for the Determination of Planar Lipid Bilayer Breakdown Voltage and Its Applications

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