“…One of the major constraints in this method has been the heavy load of microorganisms, which rapidly putrefy the tissue during storage before processing. The widely used methods such as heat sterilization (Giri et al, 2000), acid stabilization (Cai et al, 1995), and fermentation (Kherrati et al, 1998;Rao et al, 1996) could not be adopted in our scheme of protein retrieval because these methods either inactivate tissue proteinases or do not ensure complete microbial safety (Haapapuro et al, 1997). Thus, the need was felt for standardizing a suitable method for decontamination of poultry viscera, without inactivating the tissue proteinases.…”