Stabilization of SARS-CoV-2 RNA in wastewater via rapid RNA extraction

Torabi, Soroosh; Amirsoleimani, Atena; Banadaki, Mohammad Dehghan; Strike, William; Rockward, Alexus; Noble, Ann; Liversedge, Matthew; Keck, James W.; Berry, Scott M.

doi:10.1016/j.scitotenv.2023.162992

Cited by 8 publications

(2 citation statements)

References 18 publications

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“…There are several challenges that could create a barrier to entry for WBE use by rural communities [ 11 , 13 ]. In general, rural communities are isolated from environmental laboratory services which can impact their ability to obtain timely results on samples collected [ 14 , 15 ]. There also has been a limited amount of studies that instituted WBE in rural communities [ 13 , [15] , [16] , [17] , [18] , [19] ].…”

Section: Introductionmentioning

confidence: 99%

Fate-and-transport modeling of SARS-CoV-2 for rural wastewater-based epidemiology application benefit

Bognich,

Howell,

Butler

2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

Fate-and-transport modeling of SARS-CoV-2 for rural wastewater-based epidemiology application benefit

Bognich,

Howell,

Butler

2024

Heliyon

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“…The methods for SARS-CoV-2 WBE vary in efficiency and sensitivity due to local settings and to different combinations of wastewater sampling strategies and RNA extraction and preservation techniques (Le 2023 ; Li et al 2023a ; Feng et al 2023 ; Torabi et al 2023 ). Nonetheless, they have been adopted successfully in several countries on all continents with human settlements (Street et al 2020 ; Ahmed et al 2020 ; Gonzalez et al 2020 ; Arora et al 2020 ; Agrawal et al 2021b , a ; Weidhaas et al 2021 ; Bar-Or et al 2021 ; Hasan et al 2021 ; Saththasivam et al 2021 ; Carrillo-Reyes et al 2021 ; Wu et al 2021 ; Yaniv et al 2021 ; Giraud-Billoud et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

20-Month monitoring of SARS-CoV-2 in wastewater of Curitiba, in Southern Brazil

Belmonte-Lopes,

Barquilha,

Kozak

et al. 2023

Environ Sci Pollut Res

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The COVID-19 pandemic resulted in the collapse of healthcare systems and led to the development and application of several approaches of wastewater-based epidemiology to monitor infected populations. The main objective of this study was to carry out a SARS-CoV-2 wastewater based surveillance in Curitiba, Southern Brazil Sewage samples were collected weekly for 20 months at the entrance of five treatment plants representing the entire city and quantified by qPCR using the N1 marker. The viral loads were correlated with epidemiological data. The correlation by sampling points showed that the relationship between the viral loads and the number of reported cases was best described by a cross-correlation function, indicating a lag between 7 and 14 days amidst the variables, whereas the data for the entire city presented a higher correlation (0.84) with the number of positive tests at lag 0 (sampling day). The results also suggest that the Omicron VOC resulted in higher titers than the Delta VOC. Overall, our results showed that the approach used was robust as an early warning system, even with the use of different epidemiological indicators or changes in the virus variants in circulation. Therefore, it can contribute to public decision-makers and health interventions, especially in vulnerable and low-income regions with limited clinical testing capacity. Looking toward the future, this approach will contribute to a new look at environmental sanitation and should even induce an increase in sewage coverage rates in emerging countries. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-023-27926-x.

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