Stabilization of α-chymotrypsin at air–water interface through surface binding to gold nanoparticle scaffolds

Jordan, Brian J.; Hong, Rui; Gider, Basar; Hill, Jason; Emrick, Todd; Rotello, Vincent M.

doi:10.1039/b603980d

Cited by 41 publications

(29 citation statements)

References 30 publications

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“…Stability of the gold solutions can be achieved by the introduction of molecules that interact with AuNPs through bonding and/or electrostatic interactions. 58,59 For this purpose, various biological probes have been used for the stabilization and/or functionalisation of colloidal AuNPs; these include nucleic acids, enzymes, receptors, lectins, antibodies and superantigens. [60][61][62][63][64]…”

Section: Colloidal Aunps Stabilizationmentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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Gold nanoparticles (AuNPs) provide excellent platforms for the development of colorimetric biosensors as they can be easily functionalised, displaying different colours depending on their size, shape and state of aggregation. In the last decade, a variety of biosensors have been developed to exploit the extent of colour changes as nano-particles (NPs) either aggregate or disperse, in the presence of analytes. Of critical importance to the design of these methods is that the behaviour of the systems has to be reproducible and predictable. Much has been accomplished in understanding the interactions between a variety of substrates and AuNPs, and how these interactions can be harnessed as colorimetric reporters in biosensors. However, despite these developments, only a few biosensors have been used in practice for the detection of analytes in biological samples. The transition from proof of concept to market biosensors requires extensive long-term reliability and shelf life testing, and modification of protocols and design features to make them safe and easy to use by the population at large. Developments in the next decade will see the adoption of user friendly biosensors for point-of-care and medical diagnosis as innovations are brought to improve the analytical performances and usability of the current designs. This review discusses the mechanisms, strategies, recent advances and perspectives for the use of AuNPs as colorimetric biosensors.

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Section: Colloidal Aunps Stabilizationmentioning

confidence: 99%

Gold nanoparticle-based colorimetric biosensors

et al. 2018

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“…Further studies demonstrated that nanoparticle-protein complexation can considerably stabilize the bound proteins against denaturation at the air/water interface. [26] Specific biomacromolecular interactions such as streptavidin/biotin complementarity (K a $ 10 14 M…”

Section: Review Articlementioning

confidence: 99%

Applications of Nanoparticles in Biology

2008

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“…[2b,33] To examine the potential role of nanoparticle stabilisation of the functional protein in this work, we have generated and examined the impact of cellular exposure to particle formulations in which the protein was bound through a reductively [7b,27] or pH [4b,26] -cleavable linker, particle types (9) and (10). The pH or reductant-initiated cleavage of protein from these particles was initially confirmed (Figure S5 in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Engineering Cytochrome‐Modified Silica Nanoparticles To Induce Programmed Cell Death

Huang

Davies

Davis

2013

Chemistry A European J

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A low native membrane permeability and ineffective access to the cellular cytosol, together with aggressive proteolytic degradation, often severely hampers the practical application of any therapeutic protein or antibody. Through engineering the charging profile of mesoporous silica nanoparticles, cellular uptake and subsequent subcellular distribution can be controlled. We show herein that programmed cell death can subsequently be induced across a population of cancer cells with remarkable efficacy on conjugating a specific caspase-cascade-activating cytochrome to such cytosol-accessing particles.

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Stabilization of α-chymotrypsin at air–water interface through surface binding to gold nanoparticle scaffolds

Abstract: Gold nanoparticles stabilize chymotrypsin (ChT) against denaturation at the air-water interface through catenation and preferential localization of the nanoparticles at the airwater interface with concomitant decrease in interfacial energy.

Cited by 41 publications

References 30 publications

Gold nanoparticle-based colorimetric biosensors

Gold nanoparticle-based colorimetric biosensors

Applications of Nanoparticles in Biology

Engineering Cytochrome‐Modified Silica Nanoparticles To Induce Programmed Cell Death

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