Stabilized and Immobilized <i>Bacillus subtilis</i> Arginase for the Biobased Production of Nitrogen‐Containing Chemicals

Könst, Paul; Turras, Pedro M. C. C. D.; Franssen, M.C.R.; Scott, Elinor L.; Sanders, J.P.M.

doi:10.1002/adsc.201000034

Cited by 37 publications

(20 citation statements)

References 56 publications

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“…High efficient bacteria degrading aquatic pollutants as the key to the bioremediation technology has been researched for many years [10][11][12][13]. Immobilized technology, fixing complete microbial cells to a certain extent, can contribute to improving the microbial cell density, degradation and tolerance ability of free cell to toxic substances and avoiding the loss of the microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Experiments on the Effects of Dissolved Oxygen on the Free and Immobilization Effective Microorganisms (EM) in Treating Polluted River Water

Ji¹,

Zhu²,

Zhu³

et al. 2014

Advances in Intelligent Systems Research

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Abstract-The immobilization of effective microorganisms (EM)on matrix supports is of great importance for improving microbes' density and avoiding microbes flooding so as to enhance removal efficiency in polluted river water. What's more, DO is of great importance in the wastewater treatment. Based on the previous study, the effects of dissolved oxygen (DO) on the activities of EM and IAEM (minerals adsorbing EM embedded by sodium alginate) were also investigated in polluted river water. The results showed that with abundant DO, removal efficiency of NH 3 -N, TP and COD for EM and IAEM were increased by (34.34%, 18.18%, 4.59%) and (5.71%, 5.06%, 2.79%), which demonstrated EM alone was more adaptive to survive and reproduce under aerobic conditions, while immobilized microbes could better overcome the limitation of DO.

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Section: Introductionmentioning

confidence: 99%

Experiments on the Effects of Dissolved Oxygen on the Free and Immobilization Effective Microorganisms (EM) in Treating Polluted River Water

Ji¹,

Zhu²,

Zhu³

et al. 2014

Advances in Intelligent Systems Research

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“…On the other hand, cyanophycin and its derivatives are good candidates as starting material for the production of nitrogen-containing bulk chemicals and are also a source of a polyaspartic acid-like polymer with a reduced arginine content that may find applications in different areas (17,18).…”

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confidence: 99%

Increased Lysine Content Is the Main Characteristic of the Soluble Form of the Polyamide Cyanophycin Synthesized by Recombinant Escherichia coli

Frommeyer¹,

Steinbüchel

2013

Appl Environ Microbiol

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bCyanophycin, a polyamide of cyanobacterial or noncyanobacterial origin consisting of aspartate, arginine, and lysine, was synthesized in different recombinant strains of Escherichia coli expressing cphA from Synechocystis sp. strain PCC 6308 or PCC 6803, Anabaena sp. strain PCC 7120, or Acinetobacter calcoaceticus ADP1. The molar aspartate/arginine/lysine ratio of the water-soluble form isolated from a recombinant strain expressing CphA 6308 was 1:0.5:0.5, with a lysine content higher than any ever described before. The water-insoluble form consisted instead of mainly aspartate and arginine residues and had a lower proportion of lysine, amounting to a maximum of only 5 mol%. It could be confirmed that the synthesis of soluble cyanobacterial granule polypeptide (CGP) is independent of the origin of cphA. Soluble CGP isolated from all recombinant strains contained a least 17 mol% lysine. The total CGP portion of cell dry matter synthesized by CphA 6308 from recombinant E. coli was about 30% (wt/ wt), including 23% (wt/wt) soluble CGP, by using terrific broth complex medium for cultivation at 30°C for 72 h. Enhanced production of soluble CGP instead of its insoluble form is interesting for further application and makes recombinant E. coli more attractive as a suitable source for the production of polyaspartic acid or dipeptides. In addition, a new low-cost, time-saving, effective, and common isolation procedure for mainly soluble CGP, suitable for large-scale application, was established in this study.C yanophycin, a highly branched polypeptide that is contained in the cyanobacterial granule, consists of a polyaspartic acid backbone to which arginine residues are linked by isopeptide bonds at free carboxylate groups, and the polymer is therefore called multi-L-arginyl-polyaspartic acid and cyanobacterial granule polypeptide (CGP) (1, 2). The polypeptide is synthesized via ATP-, MgCl 2 -, KCl-, and sulfhydryl reagent-dependent nonribosomal protein biosynthesis by the cphA-encoded cyanophycin synthetase CphA (1, 3). The incorporation of L-arginine is dependent on the presence of L-aspartic acid and results in a branched copolymer with molecular masses ranging from 25 to 100 kDa (1, 4). The accumulation of CGP in cyanobacteria is, for example, triggered by phosphate starvation (5) and serves the cells as a nitrogen and energy storage compound (3). The ability to synthesize CGP is not a unique feature of cyanobacteria but also occurs in other groups of bacteria (6, 7). The properties of CGP concerning solubility are interesting, because it is insoluble at neutral pH but soluble at pHs of Ͻ2 and Ͼ9 (8). This makes it easy to isolate CGP with 0.1 N HCl, because after the treatment of cyanobacterial cells with acid, the only polymer isolated from extracts is a polymer consisting of arginine and aspartic acid at a molar ratio of about 1:1 (3).Heterologously synthesized CGP, e.g., from bacterial hosts like Escherichia coli, Pseudomonas putida, and Ralstonia eutropha or from yeasts, is quite different (9, 10, 11). The water...

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“…Especially di‐alcohols might be produced from glycerol. Next to oils/fats, amino acids derived from proteins can also serve as building blocks for various chemicals . In the next two sub‐sections, it is estimated how much base chemicals reported in the Eurostat database can potentially be produced from either glycerol or amino acids.…”

Section: Resultsmentioning

confidence: 99%

Raw material demand and sourcing options for the development of a bio‐based chemical industry in Europe.

Bos

Sanders

2013

Biofuels Bioprod Bioref

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This perspective presents an estimation of the future demand for biomass of the chemical industry in Europe, provided that naphtha, the present feedstock of the petrochemical industry, is fully replaced by biomass. Data are based on the Eurostat data on manufactured goods for EU27 in 2007. Two different strategies of biomass application are studied: (i) biomass is used to produce the six platform chemicals that presently form the basis of the petrochemical industry, which would allow the subsequent production of all chemicals that are presently produced in the existing infrastructure, and (ii) the chemical functionality present in the various constituents of biomass is used to produce directly the functionalized molecules that are presently produced via the platform chemicals. Not only is the use of carbohydrates and lignin taken into account, also the use of biomass components, such as proteins and natural oils, is considered. It is shown that the second approach will require less biomass (and thus land) than the first approach and that the application of biorefinery technologies, in order to separate the biomass in its different constituents, which can subsequently be used to produce the functionalized molecules, can even further diminish the demand for land. Furthermore it is concluded that production of chemicals from biomass is more beneficial that production of transportation fuels or electricity. © 2013 Society of Chemical Industry and John Wiley & Sons, Ltd

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Stabilized and Immobilized Bacillus subtilis Arginase for the Biobased Production of Nitrogen‐Containing Chemicals

Cited by 37 publications

References 56 publications

Experiments on the Effects of Dissolved Oxygen on the Free and Immobilization Effective Microorganisms (EM) in Treating Polluted River Water

Experiments on the Effects of Dissolved Oxygen on the Free and Immobilization Effective Microorganisms (EM) in Treating Polluted River Water

Increased Lysine Content Is the Main Characteristic of the Soluble Form of the Polyamide Cyanophycin Synthesized by Recombinant Escherichia coli

Raw material demand and sourcing options for the development of a bio‐based chemical industry in Europe.

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