Stabilizer‐Free Poly(lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots as a Potential Carrier Applied in Human Mesenchymal Stem Cells

Kuo, Wen-Shuo; Hwang, Shiaw-Min; Sei, Hei-Tin; Ku, Yu-Cian; Hsu, Lee-Feng; Cheng, Fong‐Yu; Hsieh, Patrick C.H.; Yeh, Chen-Sheng

doi:10.1002/jccs.200900138

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…Attempts to combine the properties of NCs with biocompatible polymer particles are still restricted to very few examples. In two recent publications, a method was introduced to externally label PLGA nanoparticles with NCs as potential biological probe for bioimaging and MRI spectroscopy [28,29]. The authors show homogenous distribution of NCs covering PLGA particle surface by TEM and are able to detect fluorescent PLGA nanoparticles by fluorescence microscopy.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy

Schliehe

Thiry

et al. 2011

Journal of Controlled Release

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Biodegradable poly-(D,L-lactide-co-glycolide) microspheres (PLGA-MS) are approved as a drug delivery system in humans and represent a promising antigen delivery device for immunotherapy against cancer. Immune responses following PLGA-MS vaccination require cross-presentation of encapsulated antigen by professional antigen presenting cells (APCs). While the potential of PLGA-MS as vaccine formulations is well established, the intracellular pathway of cross-presentation following phagocytosis of PLGA-MS is still under debate. A part of the controversy stems from the difficulty in unambiguously identifying PLGA-MS within cells. Here we show a novel strategy for the efficient encapsulation of inorganic nanocrystals (NCs) into PLGA-MS as a tool to study their intracellular localization. We microencapsulated NCs as an electron dense marker to study the intracellular localization of PLGA-MS by transmission electron microscopy (TEM) and as fluorescent labels for confocal laser scanning microscopy. Using this method, we found PLGA-MS to be rapidly taken up by dendritic cells and macrophages. Co-localization with the lysosomal marker LAMP1 showed a lysosomal storage of PLGA-MS for over two days after uptake, long after the initiation of cross-presentation had occurred. Our data argue against an escape of PLGA-MS from the endosome as has previously been suggested as a mechanism to facilitate cross-presentation of PLGA-MS encapsulated antigen.

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Section: Discussionmentioning

confidence: 99%

Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy

Schliehe

Thiry

et al. 2011

Journal of Controlled Release

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“…However, the conjugation between theQDs and thepolymers is a complex procedure, difficult to characterize in comparison to the conjugation with organic dyes [31][32][33]. In this view, still afew cases report postformulation technology as an application for the covalent linkage of QDs to polymers on theNP surface (i.e., for monitoring the nuclear translocation of NPs [34] and for targeting and labeling human mesenchimal stem cells [35]).…”

Section: Introductionmentioning

confidence: 99%

Nanoimaging: photophysical and pharmaceutical characterization of poly-lactide-co-glycolide nanoparticles engineered with quantum dots

et al. 2015

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Quantum dots (QDs) and polymeric nanoparticles (NPs) are considered good binomials for the development of multifunctional nanomedicines for multimodal imaging. Fluorescent imaging of QDs can monitor the behavior of QD-labeled NPs in both cells and animals with high temporal and spatial resolutions. The comprehension of polymer interaction with the metallic QD surface must be considered to achieve a complete chemicophysical characterization of these systems and to describe the QD optical properties to be used for their unequivocal identification in the tissue. In this study, by comparing two different synthetic procedures to obtain polymeric nanoparticles labeled with QDs, we investigated whether their optical properties may change according to the formulation methods, as a consequence of the different polymeric environments. Atomic force microscopy, transmission electron microscopy, confocal and fluorescence lifetime imaging microscopy characterization demonstrated that NPs modified with QDs after the formulation process (post-NPs-QDs) conserved the photophysical features of the QD probe. In contrast, by using a polymer modified with QDs to formulate NPs (pre-NPs-QDs), a significant quenching of QD fluorescence and a blueshift in its emission spectra were observed. Our results suggest that the packaging of QDs into the polymeric matrix causes a modification of the QD optical properties: these effects must be characterized in depth and carefully considered when developing nanosystems for imaging and biological applications.

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“…quantum dots (QD) to obtain double imaging/drug release (theranostic aim). If compared with the encapsulation process, the conjugation and the exposure of QD on NPs surface is preferred in order to avoid the QD release and to ensure the optimal imaging [12,13]. …”

mentioning

confidence: 99%

AFM/TEM Complementary Structural Analysis of Surface-Functionalized Nanoparticles

Ruozi¹

2014

J Phys Chem Biophys

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HighlightsShort collection of bullet points of the core article 1. Nanomedicine deserves interesting perspective for real application in medicine and surface modified nanocarriers represent the cutting-edge of nanomedicine.2. Aiming to the maximum translatability, surface-modified nanocarriers must be properly characterized, in terms of surface properties. Advanced microscopies (Atomic Force Microscopy andTransmission Electron Microscopy) help in better understanding of the chemico-physical features of nanocarriers 4. AFM and TEM give high resolution outcomes and should be chosen depending on specific the nature of surface-ligands 5. AFM allows to discriminate on the qualitative evaluation of ligands of different nature (QDs vs antibody) , without any additional treatment and without operating in a vacuum environment AbstractIn the field of nanomedicine, the characterization of functionalized drug delivery systems, introduced on market as efficacious and selective therapeutics, represents a pivotal aspect of great importance. In particular, the morphology of polymeric nanoparticles, the most studied nanocarriers, is frequently assessed by transmission electron microscopy (TEM). Despite of TEM high resolution and versatility, this technology is frequently hampered by both the complicated procedure for sample preparation and the operative condition of analysis. Considering the scanning probe microscopies, atomic force microscopy (AFM) represents an extraordinary tool for the detailed characterization of submicron-size structure as the surface functionalization at the atomic scale. In this paper we discussed the advantage and limits of these microscopies applied to the characterization of PLGA nanoparticles functionalized with three different kinds of ligands (carbohydrate ligand, an antibody and quantum dots crystals) intentionally designed, created and tailored with specific physico-chemical properties to meet the needs of specific applications (targeting or imaging). quantum dots (QD) to obtain double imaging/drug release (theranostic aim). If compared with the encapsulation process, the conjugation and the exposure of QD on NPs surface is preferred in order to avoid the QD release and to ensure the optimal imaging [12,13]. Graphical Abstract PEG-QDs Materials and Methods Preparation of nanoparticlesAll NPs analysed were obtained according to the nanoprecipitation procedure [14]. The functionalization can be inserted during NPs formulation by using polymer previously modified (Neu5Ac-PLGA502) or after the NPs formation by activating the functional groups onto NPs surfaces by "in situ" reaction and subsequent conjugation with ligands (Rituximab-PLGA503 NP and QD-PLGA503 NPs).Sialic acid derivative conjugated nanoparticles (Neu5Ac-PLGA502 NPs) : Initially, PLGA502H (50% lactic/50% glycolic acid ratio, inherent viscosity 0.20 dl/g, MW 4900; Boehringer-Ingelheim, Ingelheim am Rhein, Germany), selected as polymer on the basis of the yield of sugar conjugation, was conjugated with Neu5Ac (Sigma Aldrich), a sialic acid...

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Stabilizer‐Free Poly(lactide‐co‐glycolide) Nanoparticles Conjugated with Quantum Dots as a Potential Carrier Applied in Human Mesenchymal Stem Cells

Cited by 7 publications

References 32 publications

Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy

Microencapsulation of inorganic nanocrystals into PLGA microsphere vaccines enables their intracellular localization in dendritic cells by electron and fluorescence microscopy

Nanoimaging: photophysical and pharmaceutical characterization of poly-lactide-co-glycolide nanoparticles engineered with quantum dots

AFM/TEM Complementary Structural Analysis of Surface-Functionalized Nanoparticles

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