Stabilizing Effect of Four Types of Disaccharide on the Enzymatic Activity of Freeze-dried Lactate Dehydrogenase: Step by Step Evaluation from Freezing to Storage

Abstract

Taking the storage stability of freeze-dried proteins under the various conditions (temperature and RH) into consideration, trehalose is better suited as the stabilizer than other disaccharides.

Docking Studies of Stress Tolerant Proteins With Protective Molecules

“…The increase concentration above 25-30% of trehalose cause to increase water-water interactions lead to cause more stable and widely hydrogen bonded water clusters, these concentrations of sugars called as structure makers [50]. Trehalose, mannitol and lactose these combined sugars can preserve some enzyme proteins namely lactate dehydrogenase or phosphofructokinase through water replacements [51]. The present study revealed the interactions of stress tolerant proteins with trehalose sugar and these three stress tolerant enzymes possess weak interaction with trehalose sugar which was proven by inslico method using GOLD software.…”

Oligosaccharide-based Surfactant/Citric Acid Buffer System Stabilizes Lactate Dehydrogenase during Freeze-drying and Storage without the Addition of Natural Sugar

“…Meanwhile, storage under a humidifying condition of 33 RH accelerated the loss of activity. The water moisture plasticized the lyophilized matrix and increased the mobility of the components, thus accelerating the chemical reaction or degradation 4,35,36 .…”

“…It was assumed that owing to the low glass forming ability of alkyl β-D-glucosides T g , 11.4-16.3 25 , the amount of amorphous water remaining after ice freezing decreased, and an acidification in the non-frozen matrix or the formation of strong interaction between sugar-moiety and buffer components occurred. Hence, the alkyl β-Dglucosides might be hydrolyzed under freeze-concentrated state, and formed hemiacetal structure, which induces the Maillard reaction that loss the LDH activity even in the lyophilized condition 35 . Alternatively, the formation of specific interaction between alkyl β-D-glucoside and citric acid buffer can be assumed, as observed for the lyophilized system consisted of natural sugar and phosphate buffer 38 .…”

Stabilizing Effect of β-Cyclodextrin on Limaprost, a PGE₁ Derivative, in Limaprost Alfadex Tablets (Opalmon®) in Highly Humid Conditions

“…15) However, maltose, trehalose and mannitol had no stabilizing effect under high humidity conditions, whereas dextran suppressed the degradation, i.e., the appearances of 11-deoxy-Δ 10 after stored at 25°C, 75% relative humidity (R.H.) for two weeks were 3.4, 3.7, 5.5, 17.7 and 1.2% for Limaprost alfadex alone and its composites with maltose, trehalose, mannitol and dextran, respectively. Trehalose did not improve the chemical stability of Limaprost, probably because trehalose is reported to stabilize mainly thermal degradations, [16][17][18][19][20][21] but not water-catalyzed degradations, and its stabilizing ability decreases under higher humidity conditions, as demonstrated in the insulin/ trehalose system. 22) On the other hand, dextran improved the stability of Limaprost alfadex, [23][24][25][26][27] which is utilized for the new formulation of Opalmon ® tablets since 2006.…”