Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

Teh, Shia-Yen; Khnouf, Ruba; Fan, Z. Hugh; Lee, Abraham P.

doi:10.1063/1.3665221

Cited by 137 publications

(143 citation statements)

References 39 publications

(43 reference statements)

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“…We evaluated the level of the surface hydrophilicity induced by two commercially available PVA solutions commonly used in PDMS surface modification [27][28][29][32][33][34] . We applied PVA solutions with 87-90% and 99+% hydrolysis degrees on PDMS surfaces that were plasma oxidized under the same conditions (50 W for 1 min) and concluded that the former had a more profound effect on the PDMS surface chemistry both in the short and the long term (Figure 1a).…”

Section: Resultsmentioning

confidence: 99%

“…Our experimental data show that the PVA-treated PDMS surfaces retain their hydrophilicity in the long term. We leveraged PVA solutions with two distinct hydrolysis degrees (87-90% and 99+%) because these are widely used for surface modification in PDMSbased microfluidics 33,34 . Based on a wettability study, we found that PVA with a hydrolysis degree of 87-90% was more efficient in both the short and long term compared with PVA with a 99+% hydrolysis degree.…”

Section: Discussionmentioning

confidence: 99%

“…There is one elegant example of using PVA-modified surfaces in droplet microfluidics for the controlled generation of lipid vesicles 34 . Although the aforementioned examples demonstrate the potential of the PVA deposition strategy for chemistry and biology, a systematic characterization and optimization of this technique for droplet microfluidics is lacking, specifically regarding the effects of the various process parameters on the surface properties.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition

et al. 2017

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Polydimethylsiloxane (PDMS) is a dominant material in the fabrication of microfluidic devices to generate water-in-oil droplets, particularly lipid-stabilized droplets, because of its highly hydrophobic nature. However, its key property of hydrophobicity has hindered its use in the microfluidic generation of oil-in-water droplets, which requires channels to have hydrophilic surface properties. In this article, we developed, optimized, and characterized a method to produce PDMS with a hydrophilic surface via the deposition of polyvinyl alcohol following plasma treatment and demonstrated its suitability for droplet generation. The proposed method is simple, quick, effective, and low cost and is versatile with respect to surfactants, with droplets being successfully generated using both anionic surfactants and more biologically relevant phospholipids. This method also allows the device to be selectively patterned with both hydrophilic and hydrophobic regions, leading to the generation of double emulsions and inverted double emulsions.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition

et al. 2017

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“…It has been reported that the use of F-68 in liposomes can greatly improve the stability of liposomes. 64 More than 80% percent of liposome are present after storage for two days. Consequently, we believe that the presence of F-68 outside the liposomes does not hinder the application of the liposomes, but provides more advantages, such as easier fabrication and better stability.…”

mentioning

confidence: 99%

Monodisperse Uni- and Multicompartment Liposomes

Deng¹,

Yelleswarapu²,

Huck³

2016

J. Am. Chem. Soc.

230

228

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Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great detail. As a result, high throughput, high-yielding routes to monodisperse liposomes with multiple compartments have not been demonstrated. Here, we report on a surfactant-assisted microfluidic route to uniform, single bilayer liposomes, ranging from 25 µm to 190 µm, and with or without multiple inner compartments. The key of our method is the precise control over the developing interfacial energies of complex W/O/W emulsion systems during liposome formation, which is achieved via an additional surfactant in the phase. The liposomes consist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluoresce microscopy, and they can act as compartments for cell-free gene expression. The microfluidic technique can be expanded to create liposomes with a multitude of coupled compartments, opening routes to networks of multistep microreactors.There has been a significant interest in the use of liposomes, self-assembled phospholipid vesicles composed of bilayer membranes, in fields as diverse as targeted drug delivery, 1,2 membrane protein science, 3-5 bioreactors 6-8 and biosensors. 9,10 Cell-sized liposomes that encapsulate biomolecules and incorporate biological functions provide a versatile mimic of certain aspects of living cells, as exemplified by work showing RNA replication, [11][12][13] in vitro transcription and translation of gene networks, 6,14-16 and organization of cell division machinery in liposomes. [17][18][19][20][21][22][23][24]

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“…1 A subcategory of microfluidics in which two or more immiscible fluids are introduced to create discrete volumes, these systems have been studied extensively in the past decade to form simple droplets (oil-in-water or water-in-oil), 2,3 double emulsions, 4-7 irregular particles (e.g., Janus particles), 8,9 and lipid vesicles. 10 Continuous emulsion streams are formed in microfluidic systems through specialized geometries-perhaps the most common of which are co-flowing streams, cross-flowing streams, and hydrodynamic flow-focusing 11 -with a primary advantage over conventional methods being precise control of the size and composition of the emulsion.…”

Section: Introductionmentioning

confidence: 99%

Parallel generation of uniform fine droplets at hundreds of kilohertz in a flow-focusing module

et al. 2013

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Droplet-based microfluidic systems enable a variety of biomedical applications from point-of-care diagnostics with third world implications, to targeted therapeutics alongside medical ultrasound, to molecular screening and genetic testing. Though these systems maintain the key advantage of precise control of the size and composition of the droplet as compared to conventional methods of production, the low rates at which droplets are produced limits translation beyond the laboratory setting. As well, previous attempts to scale up shear-based microfluidic systems focused on increasing the volumetric throughput and formed large droplets, negating many practical applications of emulsions such as site-specific therapeutics. We present the operation of a parallel module with eight flow-focusing orifices in the dripping regime of droplet formation for the generation of uniform fine droplets at rates in the hundreds of kilohertz. Elevating the capillary number to access dripping, generation of monodisperse droplets of liquid perfluoropentane in the parallel module exceeded 3.69 Â 10 5 droplets per second, or 1.33 Â 10 9 droplets per hour, at a mean diameter of 9.8 lm. Our microfluidic method offers a novel means to amass uniform fine droplets in practical amounts, for instance, to satisfy clinical needs, with the potential for modification to form massive amounts of more complex droplets. V C 2013 AIP Publishing LLC. [http://dx

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Stable, biocompatible lipid vesicle generation by solvent extraction-based droplet microfluidics

Cited by 137 publications

References 39 publications

Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition

Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition

Monodisperse Uni- and Multicompartment Liposomes

Parallel generation of uniform fine droplets at hundreds of kilohertz in a flow-focusing module

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