Stable disruption of ethanol production by deletion of the genes encoding alcohol dehydrogenase isozymes in Saccharomyces cerevisiae

Ida, Yoshihiro; Furusawa, Chikara; Hirasawa, Takashi; Shimizu, Hiroshi

doi:10.1016/j.jbiosc.2011.09.019

Cited by 48 publications

(40 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To that end, we analyzed the ADH1-disrupted strain of S. cerevisiae and examined the culture characteristics of the constructed strain. 6 As expected, ethanol yield in the ADH1-disrupted strain was lower than that in the parental strain. Moreover, glycerol yield in the ADH1-disrupted strain was higher than that in the parent strain, suggesting that oxidation of NADH produced by glycolysis was oxidized by glycerol biosynthesis reactions, as a substitute to ethanol biosynthesis pathways.…”

Section: Characteristics Of S Cerevisiae Recombinant Strains Incapabsupporting

confidence: 69%

“…6 The ethanol production levels became low at the initial steady-state by 100 h. However, ethanol production levels gradually increased after reaching the initial steady-state. The ethanol production yield was increased, while the glycerol production yield was decreased, indicating that NADH produced via glycolysis was oxidized by the remaining ethanol biosynthesis reactions in the ADH1-disrupted strain under oxygen-limited conditions rather than by glycerol biosynthesis reactions.…”

Section: Characteristics Of S Cerevisiae Recombinant Strains Incapabmentioning

confidence: 97%

“…6 However, complete loss of ethanol production ability in this recombinant strain could not be achieved, and glycerol production Potential of a Saccharomyces cerevisiae recombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction was enhanced under anaerobic conditions. Therefore, all NADH-dependent ethanol biosynthesis pathways and glycerol biosynthesis pathways were disrupted in S. cerevisiae.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Potential of aSaccharomyces cerevisiaerecombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction

et al. 2013

Self Cite

View full text Add to dashboard Cite

Saccharomyces cerevisiae shows high growth activity under low pH conditions and can be used for producing acidic chemicals such as organic acids as well as fuel ethanol. However, ethanol can also be a problematic by-product in the production of chemicals except for ethanol. We have reported that a stable low-ethanol production phenotype was achieved by disrupting 6 NADHdependent alcohol dehydrogenase genes of S. cerevisiae. Moreover, the genes encoding the NADH-dependent glycerol biosynthesis enzymes were further disrupted because the ADH-disrupted recombinant strain showed high glycerol production to maintain intracellular redox balance. The recombinant strain incapable producing ethanol and glycerol could have the potential to be a host for producing metabolite(s) whose biosynthesis is coupled with NADH oxidation. Indeed, we successfully achieved almost 100% yield for L-lactate production using this recombinant strain as a host. In addition, the potential of our constructed recombinant strain for efficient bioproduction, particularly under anaerobic conditions, is also discussed.

show abstract

Section: Characteristics Of S Cerevisiae Recombinant Strains Incapabsupporting

confidence: 69%

Section: Characteristics Of S Cerevisiae Recombinant Strains Incapabmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Potential of aSaccharomyces cerevisiaerecombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have reported that disruption of genes encoding NADH-dependent alcohol dehydrogenases, which convert acetaldehyde to ethanol, is important for elimination of the ethanol biosynthesis pathways. 50,51) Hence, we examined the effect of disrupting the ADH1-5 genes on the production of succinic acid by S. cerevisiae in which the SDH1 and SDH2 genes were disrupted ( Fig. 2 and Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…2 and Table 2). The SDH1 and SDH2 genes were disrupted in ADH1-5-disrupted strain S149 50) and the resulting strain was named S149sdh12.…”

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling

Ito

Hirasawa

Shimizu

2014

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.

show abstract

Synthetic Saccharomyces cerevisiae‐Shewanella oneidensis consortium enables glucose‐fed high‐performance microbial fuel cell

Lin

Bai

et al. 2016

AIChE Journal

View full text Add to dashboard Cite

Microbial fuel cells (MFCs) were green and sustainable bio‐electrochemical reactors for simultaneous wastewater treatment and electricity harvest from organic wastes. However, exoelectrogens, such as Shewanella and Geobacter being widely studied in MFCs, could only use a limited spectrum of carbon sources. To expand the carbon source range being used in MFCs, we herein rationally designed a glucose‐fed fungus‐bacteria microbial consortium including a fermenter (Saccharomyces cerevisiae) in which the ethanol pathway was knocked out and the lactic acid biosynthesis pathway from Bovin was introduced into S. cerevisiae, and an exoelectrogen (Shewanella oneidensis MR‐1). We optimized the co‐culturing conditions of the microbial consortium to achieve an optimal coordination between carbon source metabolism of the fermenter and extracellular electron transfer of the exoelectrogen, such that lactate, the metabolic product of glucose by the recombinant S. cerevisiae, was continuously supplied to S. oneidensis in a constant level until glucose exhaustion. This metabolic coordination between the fermenter and the exoelectrogen enabled bioelectricity production in a glucose‐fed MFC. Furthermore, a porin protein encoded by oprF gene from Pseudomonas aeruginosa was incorporated into the outer membrane of S. oneidensis to enhance membrane permeability and its hydrophobicity, which in turn facilitated its biofilm formation and power generation. The glucose‐fed MFC inoculated with the recombinant S. cerevisiae‐recombinant S. oneidensis generated a maximum power density of 123.4 mW/m2, significantly higher than that of recombinant S. cerevisiae‐wild‐type S. oneidensis (71.5 mW/m2). Our design strategy of synthetic microbial consortia was highly scalable to empower the possibility of a wide range of carbon sources being used in MFCs, e.g., xylose, cellulosic biomass, and recalcitrant wastes. © 2016 American Institute of Chemical Engineers AIChE J, 63: 1830–1838, 2017

show abstract

Stable disruption of ethanol production by deletion of the genes encoding alcohol dehydrogenase isozymes in Saccharomyces cerevisiae

Cited by 48 publications

References 20 publications

Potential of aSaccharomyces cerevisiaerecombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction

Potential of aSaccharomyces cerevisiaerecombinant strain lacking ethanol and glycerol biosynthesis pathways in efficient anaerobic bioproduction

Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling

Synthetic Saccharomyces cerevisiae‐Shewanella oneidensis consortium enables glucose‐fed high‐performance microbial fuel cell

Contact Info

Product

Resources

About