2003
DOI: 10.5897/ajb2003.000-1044
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Stable gene transformation in cowpea (Vigna unguiculata L. walp.) using particle gun method

Abstract: We investigated the possibility of transforming and obtaining transgenic cowpea (Vigna unguiculata L Walp) plants using the particle bombardment process. Meristematic explants that could give rise to whole fertile plants were used in transformation experiments with reporter and selectable marker genes driven by a 35S CaMV promoter. Conditions for optimal delivery of DNA to explants were established based on transient gus expression assays two days after bombardment. The size of microcarriers, microflight dista… Show more

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Cited by 41 publications
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