Stable Hydrogen Isotope Analysis of Bat Hair as Evidence for Seasonal Molt and Long-Distance Migration

Origins and migratory patterns of bats killed by wind turbines in southern Alberta: evidence from stable isotopes

“…Stable-isotope analysis of bat tissue has been used in numerous dietary studies (Fleming et al 1993, Voigt and Kelm 2006, Popa-Lisseanu et al 2007, Painter et al 2009, Cryan et al 2012, and also to investigate the origins and migratory movements of bats. (Cryan et al 2004, Cryan et al 2014. Combining this relationship with capture and museum records demonstrated that some hoary bats migrate long-distances (.2000 km; Cryan et al 2004).…”

“…(Cryan et al 2004, Cryan et al 2014. Combining this relationship with capture and museum records demonstrated that some hoary bats migrate long-distances (.2000 km; Cryan et al 2004). Further studies have attempted to evaluate the use of stable-isotope analysis to assign origins to individuals of various bat species across Europe , the eastern United States (Britzke et al 2009, Sullivan et al 2012, and Canada (Fraser et al 2012 (Britzke et al 2009).…”

Hair Sampling Techniques for River Otters

“…Hair samples from both carcasses and traps were rinsed 3 times with 2:1 chloroform : methanol solution to remove surface oils (Cryan et al 2004), dried for 72 h at 60uC, and homogenized with surgical scissors. Fecal samples were dried and ground to a fine powder.…”

“…Because dD values of precipitation (Bowen et al 2005;Hobson 1999) exhibit predictable latitudinal gradients, the 2 H signature of animal tissue can be assigned to a geographical location, allowing the identification of wintering grounds or the quantification of long-distance migration events. Measur-ing the dD values of animal tissue and relating it back to global isoclines of dD values has been successfully used for volant animals such as butterflies (Dockx et al 2004), passerines (Hobson and Wassenaar 1997), and microchiropterans (Cryan et al 2004).…”

Spatial and temporal expression of vegetation and atmospheric variability from stable carbon and nitrogen isotope analysis of bat guano in the southern United States

“…Migratory insects can bring vegetative information about other geographic locations (Wassenaar and Hobson, 1998;Lee and McCracken, 2005), sometimes in large numbers, and agricultural areas will bring insects containing vegetation information not directly related to the local climate (Lee and McCracken, 2005). Bats are known to capture insects over agricultural areas and at high altitudes, and bulk guano contains not only insect chitin, but bat hair that may not have formed contemporaneously with other components in the guano (Cryan et al, 2004). Moreover, bats are seasonal residents in Arizona and Texas, and year-round residents in California (Cockrum, 1969) and therefore collected guano will be influenced by the degree of seasonality in the vegetation d 13 C values.…”