Stable Immobilization of an Oligonucleotide Probe on a Gold Substrate Using Tripodal Thiol Derivatives

Sakata, Toshiya; Maruyama, Sumio; Ueda, Aiko; Otsuka, Hidenori; Miyahara, Yuji

doi:10.1021/la0616193

Cited by 62 publications

(66 citation statements)

References 19 publications

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“…We have already confirmed the usefulness of the oligoamine anchor 15,16,26,27,56,[72][73][74][75][76] instead of the sulfanyl group anchor [77][78][79] for molecular immobilization on gold surfaces and developed polyamineended PEG-modified gold surfaces using PEG-block-poly [2- Densely PEG-chain-tethered biointerface Y Nagasaki platform for ssDNA immobilization and detection; 73 in our scheme, PEG-b-PAMA was immobilized onto the gold surface followed by treatment with a ssDNA-SH, resulting in the formation of ssDNA-SH/ PEG-b-PAMA co-immobilized gold surface.…”

Section: Construction Of Oligodna/peg Hybrid Surfaces and Their Perfomentioning

confidence: 59%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

116

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Non-biofouling surfaces constitute one of the most important subjects in sensitively and selectively detecting biomolecular events. Poly(ethylene glycol) (PEG) chains tethered on substrate surfaces are well known to reduce non-biofouling characteristics. Protein adsorption onto a PEG-chain-tethered surface is strongly influenced by the density of the PEG chain and is almost completely suppressed by the successive treatment of longer PEG chains (5 kDa) followed by the treatment of PEG (2 kDa; mixed-PEG-chain-tethered surface) because of a significant increase in PEG chain density. To modify versatile substrate surface, PEG possessing pentaethylenehexamine at one end (N6-PEG) was prepared via a reductive amination reaction of aldehyde-ended PEG with pentaethylenehexamine. Using N6-PEG, antibody/PEG co-immobilization was conducted on a substrate possessing active ester groups. After the antibody was immobilized on the surface, PEG tethered chains were constructed surrounding the immobilized antibody. It is interesting to note that the PEG-chain-tethered functions not only as a non-fouling agent but also improves immune response. The hybrid surface was also applied to oligo DNA immobilization. The oligo DNA/PEG hybrid surface improved hybridization, retaining its non-fouling ability. Densely packed PEG tethered chains surrounding antibodies and/or oligo DNA improved their orientation on the surface. Thus, this material is promising as a high-performance biointerface for versatile applications. Keywords: antibody; biosensing; DNA; end-functionalized poly(ethylene glycol); enzyme-linked immunosorbent assay; hybrid biointerface; soft interface INTRODUCTION Specific biorecognition on substrate surfaces has long been utilized in many applications such as biosensing, 1 immunodiagnostics, 2 enzyme immunoassay, 3 western blotting 4 and protein microarrays. 5 Important factors for the improvement in specific biorecognition on surfaces are the suppression of nonspecific biofouling and the suitable orientation of immobilized biomolecules. The former decreases nonspecific noise and the background level, and the latter increases the specific recognition level. To improve the non-biofouling character of surfaces, numerous efforts have been undertaken. In general, natural polymers such as albumin, casein and dextran have been used for blocking on substrate surfaces. 6 These treatments work well and suppress protein biofouling extensively. If extremely high performance is required, however, these blocking treatments are not enough. In addition, natural products, especially those derived from animals, may have undesired contents such as bacteria and viruses, which may affect the user. 7 Under these circumstances, synthetic polymers have been recently suggested as suitable surface blocking agents. Numerous types of water-soluble polymers, such as poly(acrylamide), poly(vinyl alcohol), poly(vinyl pyrrolidone) and poly(ethylene glycol) (PEG), have been investigated so far. Recently, new types of synthetic polymers such as poly(methoxy ...

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Section: Construction Of Oligodna/peg Hybrid Surfaces and Their Perfomentioning

confidence: 59%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

116

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“…7 This is most commonly accomplished by employing mixed monolayers with short-chain spacer molecules. 5,6,[8][9][10][11][12][13][14][15][16][17] More modern approaches to enforce a sufficient free volume employ specially designed molecules, which feature additional bulky spacer groups 6,[18][19][20][21] or molecular tripods [22][23][24][25] as basis. Nevertheless, these types of adlayers also exhibit intrinsic disadvantages such as a comparable low structural order or lack of temporal stability due to phase separation.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of triazatriangulenium-based functional adlayers on Au(111) surfaces

Kühn

Baisch

Jung

et al. 2010

Phys. Chem. Chem. Phys.

104

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EditorialInterfacial systems chemistry: out of the vacuum-through the liquid-into the cell Phys. Chem Detailed scanning tunneling microscopy studies of the attachment of freestanding molecular functions to Au(111) surfaces via self-assembly of functional molecules based on triazatriangulenium platforms are presented. As shown for molecules with side chains of different length and phenyl, azobenzyl, or azobenzyl derivatives with different terminal groups (iodo, cyano, or dimethyl) as functional units, this approach allows the preparation of very stable, hexagonally ordered adlayers. The intermolecular spacings in these adlayers are independent of the attached functions with the latter being orientated perpendicular to the Au surface. Due to their open structure, adlayers of platforms with attached functional groups exhibit a tendency towards bilayer formation, which can be suppressed by derivatization with appropriate terminal groups.

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“…In order to solve this issue, several approaches, such as an introduction of more than two thiol-anchoring groups at the polymer chain ends or the shell crosslinking of polymer micelles surrounding the Au NPs, have been proposed previously. [19][20][21][22] Nonetheless, these methods were limited by complicated fabrication steps or insufficient thermal stability of resulting NPs.…”

Section: Introduction Research On Inorganic Nanoparticles (Nps)mentioning

confidence: 99%

Design and fabrication of thermally stable nanoparticles for well‐defined nanocomposites

Yoo

Kim

Bang

2013

J Polym Sci B Polym Phys

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The nanocomposites consisted of polymer and nanoparticles (NPs) have been regarded as one of core materials in the nanotechnology. From the practical viewpoint, the heat treatment is often required in many nanocomposite fabrication processes. However, some NPs such as gold NPs exhibit the low thermal stability due to the dissociation of ligands from the nanoparticle surface at elevated temperature, limiting their use in many applications. Herein, we provide an overview of the recent efforts in strategies for the design and fabrication of inorganic NPs which have enhanced thermal stability. The recent investigation on the phase behavior of thermally stable NPs within the polymer matrices (polymer blends and block copolymer), morphologies of nanocomposites induced by NPs, and examples of their applications are also discussed. These approaches may provide useful strategy to employ the NPs for the fabrication of nanocomposites in diverse applications especially where heat treatment are required.

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Stable Immobilization of an Oligonucleotide Probe on a Gold Substrate Using Tripodal Thiol Derivatives

Cited by 62 publications

References 19 publications

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Self-assembly of triazatriangulenium-based functional adlayers on Au(111) surfaces

Design and fabrication of thermally stable nanoparticles for well‐defined nanocomposites

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