Stable isotope analysis of plant-derived nitrate – Novel method for discrimination between organically and conventionally grown vegetables

Mihailova, Alīna; Pedentchouk, Nikolai; Kelly, Simon

doi:10.1016/j.foodchem.2014.01.020

Cited by 37 publications

(37 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover we considered δ 15 N and δ 13 C values of bulk tissue wheat grain samples, taking the data 344 from the literature for DK1 and measuring the samples from IT to conduce a direct comparison with 345 the compound specific data.Figure 3shows the distribution of δ 15 N and δ13 C values of Danish and 346 Italian bulk tissue samples, while Figure 4 shows the same distribution for δ 15 N and δ 13 C values of 347 Glx. The bulk tissue analysis allowed separation between wheat plants grown with the use of animal 348 manure (OSA) from plants grown using synthetic fertilizers (CS), but it was impossible to 349 distinguish the agricultural system based on legumes as green manures (OSB) from the 350 conventional one, as discussed above.…”

mentioning

confidence: 83%

“…11 Thus, the use of legumes represents a common and 54 valuable strategy for improving soil fertility in organic agriculture. However, legumes have a 55 nitrogen isotopic composition similar to that of synthetic fertilisers 1 Also the stable isotope ratio of carbon 13 C/ 12 C (expressed as δ 13 C) has also been investigated for 66 organic authentication. In C3 plants, δ 13 C is affected by water availability and drought stress, but 67 also shows significant interactions with nutrient availability and fertilization 14,15 , which frequently 68 can be systematically different between organic and conventional systems.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Paolini

Ziller

Laursen

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

We present a study deploying compound-specific nitrogen and carbon isotope analysis of amino acids to discriminate between organically and conventionally grown plants. We focused on grain samples of common wheat and durum wheat grown using synthetic nitrogen fertilizers, animal manures, or green manures from nitrogen-fixing legumes. The measurement of amino acid δ(15)N and δ(13)C values, after protein hydrolysis and derivatization, was carried out using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Our results demonstrated that δ(13)C of glutamic acid and glutamine in particular, but also the combination of δ(15)N and δ(13)C of 10 amino acids, can improve the discrimination between conventional and organic wheat compared to stable isotope bulk tissue analysis. We concluded that compound-specific stable isotope analysis of amino acids represents a novel analytical tool with the potential to support and improve the certification and control procedures in the organic sector.

show abstract

mentioning

confidence: 83%

mentioning

confidence: 99%

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Paolini

Ziller

Laursen

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Since then, the application has been expanded widely to accommodate isotopic analysis of NO − 3 in fresh water (e.g., groundwater, stream water, precipitation), soil and sediment water, soil extracts, as well as dissolved organic N (DON) in seawater and DON bound to diatoms as described by Koba et al (2010a) and McIlvin and Casciotti (2011), respectively. This method has recently been used for measurements of NO − 3 in natural plants and crops (Liu et al, 2012a, 2013a; Laursen et al, 2013; Bloom et al, 2014; Mihailova et al, 2014). The established protocol facilitates the Δ 17 O (Δ 17 O = [1 + δ 17 O] / [1 + δ 18 O] 0.5247 − 1; Kaiser et al, 2007) analysis of leaf NO − 3 to diagnose atmosphere-derived NO − 3 in leaf uptake (Mukotaka, 2014).…”

Section: Denitrifier Methods For No−3 In Natural Plantsmentioning

confidence: 99%

Nitrate dynamics in natural plants: insights based on the concentration and natural isotope abundances of tissue nitrate

et al. 2014

View full text Add to dashboard Cite

The dynamics of nitrate (NO−3), a major nitrogen (N) source for natural plants, has been studied mostly through experimental N addition, enzymatic assay, isotope labeling, and genetic expression. However, artificial N supply may not reasonably reflect the N strategies in natural plants because NO−3 uptake and reduction may vary with external N availability. Due to abrupt application and short operation time, field N addition, and isotopic labeling hinder the elucidation of in situ NO−3-use mechanisms. The concentration and natural isotopes of tissue NO−3 can offer insights into the plant NO−3 sources and dynamics in a natural context. Furthermore, they facilitate the exploration of plant NO−3 utilization and its interaction with N pollution and ecosystem N cycles without disturbing the N pools. The present study was conducted to review the application of the denitrifier method for concentration and isotope analyses of NO−3 in plants. Moreover, this study highlights the utility and advantages of these parameters in interpreting NO−3 sources and dynamics in natural plants. We summarize the major sources and reduction processes of NO−3 in plants, and discuss the implications of NO−3 concentration in plant tissues based on existing data. Particular emphasis was laid on the regulation of soil NO−3 and plant ecophysiological functions in interspecific and intra-plant NO−3 variations. We introduce N and O isotope systematics of NO−3 in plants and discuss the principles and feasibilities of using isotopic enrichment and fractionation factors; the correlation between concentration and isotopes (N and O isotopes: δ18O and Δ17O); and isotope mass-balance calculations to constrain sources and reduction of NO−3 in possible scenarios for natural plants are deliberated. Finally, we offer a preliminary framework of intraplant δ18O-NO−3 variation, and summarize the uncertainties in using tissue NO−3 parameters to interpret plant NO−3 utilization.

show abstract

“…Stable nitrogen ratios have been used to represent nitrogen cycling efficiency within plants following land use and land cover changes (Robinson 2001, Perakis et al 2015, and has been used to both evaluate interspecific competition between tree species (Hagan & Jose 2011) and differentiate between organic and synthetic nitrogen uptake (Mihailova et al 2014). Decreases in annual rainfall have been linked to a concomitant increase in foliar δ 15 N in some tree species.…”

Section: Introductionmentioning

confidence: 99%

Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai’i

Lawson

Pike

2017

Ann. For. Res.

View full text Add to dashboard Cite

Lawson S. S., Pike C. C. 2017. Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai'i. Ann. For. Res. 60(2): 279-295.Abstract. Stable carbon and nitrogen isotopes can be influenced by a multitude of factors including elevation, precipitation rate, season, and temperature. This work examined variability in foliar stable carbon (δ13C) and nitrogen (δ15N) isotope ratios of koa (Acacia koa) across 17 sites on Hawai'i Island, delineated by elevation and precipitation gradients. Sites were identified and grouped with respect to mean annual precipitation (MAP), mean annual temperature (MAT) and position along three elevation transects. Analysis of resultant δ13C and δ15N isotope ratios from multiple individuals across ecoregions indicated certain regions exhibited a correlation between carbon and/or nitrogen content, isotope ratios, precipitation, and elevation however, many comparisons showed no correlation. We used publicly available temperature and moisture data to help eliminate confounding effects by climatic drivers and capture possible points of contention. At sites where the temperature, precipitation, and elevation data were not significantly different, we compared stable isotope information to determine if additional variables might have contributed to a lack of definitive data. Our results identified several areas within the Waiakea Forest Reserve and Volcanoes National Park where, based on isotope analyses, reforestation efforts could be most successfully initiated.

show abstract

Stable isotope analysis of plant-derived nitrate – Novel method for discrimination between organically and conventionally grown vegetables

Cited by 37 publications

References 22 publications

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Nitrate dynamics in natural plants: insights based on the concentration and natural isotope abundances of tissue nitrate

Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai’i

Contact Info

Product

Resources

About

Stable isotope analysis of plant-derived nitrate – Novel method for discrimination between organically and conventionally grown vegetables

Cited by 37 publications

References 22 publications

Compound-Specific δ15N and δ13C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Compound-Specific δ15N and δ13C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Nitrate dynamics in natural plants: insights based on the concentration and natural isotope abundances of tissue nitrate

Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai’i

Contact Info

Product

Resources

About

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat

Compound-Specific δ¹⁵N and δ¹³C Analyses of Amino Acids for Potential Discrimination between Organically and Conventionally Grown Wheat