Stable isotope-assisted metabolite profiling reveals new insights into L-tryptophan chemotrophic metabolism of Rubrivivax benzoatilyticus

Recent Advances in Current Uptake Situation, Metabolic and Nutritional Characteristics, Health, and Safety of Dietary Tryptophan

“…In vitro , the bacteria Rubrivivax benzoatilyticus JA2, which metabolizes Trp as the sole source of nitrogen, was found to have multiple catabolic pathways for l -Trp through chemotrophic catabolism, where isotope tracing test revealed the significant differences in the catabolism of l -Trp in the convergent state when compared to photocatabolism . The absorption of Trp in the intestine of organisms is primarily facilitated by B 0 AT1, which is encoded by SLC6A19 .…”

“…In vitro, the bacteria Rubrivivax benzoatilyticus JA2, which metabolizes Trp as the sole source of nitrogen, was found to have multiple catabolic pathways for L-Trp through chemotrophic catabolism, where isotope tracing test revealed the significant differences in the catabolism of L-Trp in the convergent state when compared to photocatabolism. 29 The absorption of Trp in the intestine of organisms is primarily facilitated by B 0 AT1, which is encoded by SLC6A19. 30 Trp also serves as a substrate for protein synthesis and can be metabolized through three main pathways, namely the kynurenine pathway (KP), the 5-hydroxytryptamine (5-HT) pathway, and the microbial indole pathway as illustrated in Figure 1a 3.1.…”

Indoles and the advances in their biotechnological production for industrial applications

“…Similarly, uniformly 13 C-labelled glucose-13 C 6 will allow access to indoles that only carry 13 C-labelled carbon atoms and the use of position-13 C-labelled glucose will yield indoles with certain carbon atoms fully 13 C-labelled, whilst others remain unlabelled. Thus, the fate of indole or of the indole core of l-Trp can be followed in subsequent biosynthesis reactions such as gramine biosynthesis in barley [113], the auxin network in plants [114] or in the anoxygenic photosynthetic bacterium Rubrivivax benzoatilyticus [115]. Since the fermentative production of brominated indoles was established [94], it is also possible to prepare radiolabelled 77 Br-indoles, e.g.…”