Stable isotope labeling combined with liquid chromatography-tandem mass spectrometry for comprehensive analysis of short-chain fatty acids

Zheng, Jie; Zheng, Shu-Jian; Cai, Wen-Jing; Yu, Lei; Yuan, Bi-Feng; Feng, Yu‐Qi

doi:10.1016/j.aca.2019.04.021

Cited by 53 publications

(32 citation statements)

References 41 publications

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“…Lactic acid accumulation was observed in the model group with memory impairment, and this accumulation might have been observed due to decreased pyruvate dehydrogenase activity, which results in decreased catabolism of lactic acid and reduced mitochondrial respiration and thereby mitochondrial failure. Mitochondrial failure results in intracellular pH changes, synaptic dysfunction, amyloid deposition, and ultimately apoptosis [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Wang

Guo

et al. 2021

BioMed Research International

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Aging affects the brain function in elderly individuals, and Dushen Tang (DST) is widely used for the treatment of senile diseases. In this study, the protective effect of DST against memory impairment was evaluated through the Morris water maze (MWM) test and transmission electron microscopy (TEM). A joint analysis was also performed using LC-MS metabolomics and the microbiome. The MWM test showed that DST could significantly improve the spatial memory and learning abilities of rats with memory impairment, and the TEM analysis showed that DST could reduce neuronal damage in the hippocampus of rats with memory impairment. Ten potential biomarkers involving pyruvate metabolism, the synthesis and degradation of ketone bodies, and other metabolic pathways were identified by the metabolomic analysis, and it was found that 3-hydroxybutyric acid and lactic acid were involved in the activation of cAMP signaling pathways. The 16S rDNA sequencing results showed that DST could regulate the structure of the gut microbiota in rats with memory impairment, and these effects were manifested as changes in energy metabolism. These findings suggest that DST exerts a good therapeutic effect on rats with memory impairment and that this effect might be mainly achieved by improving energy metabolism. These findings might lead to the potential development of DST as a drug for the treatment of rats with memory impairment.

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Section: Discussionmentioning

confidence: 99%

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Wang

Guo

et al. 2021

BioMed Research International

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“…Additionally, over 30 metabolic pathways possibly related to amyloid deposition and ultrastructural anomalies were detected in intestine samples of the AD group [95]. Zheng et al [96] have introduced a method of stable isotope labelling and liquid chromatography-tandem mass spectrometry to sensitively detect 21 SCFAs in mice faecal samples of AD and WT mice. In an AD mouse model, decreased levels of PA, isobutyric acid, 3-hydroxybutyric acid, and 3hydroxyisovaleric acid were detected while increased levels of lactic acid, 2-hydroxybutyric acid, 2-hydroxyisobutyric acid, levulinic acid and valproic acid were found.…”

Section: Scfas and Alzheimer's Diseasementioning

confidence: 99%

The Role of Gut Bacterial Metabolites in Brain Development, Aging and Disease

2021

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In the last decade, emerging evidence has reported correlations between the gut microbiome and human health and disease, including those affecting the brain. We performed a systematic assessment of the available literature focusing on gut bacterial metabolites and their associations with diseases of the central nervous system (CNS). The bacterial metabolites short-chain fatty acids (SCFAs) as well as non-SCFAs like amino acid metabolites (AAMs) and bacterial amyloids are described in particular. We found significantly altered SCFA levels in patients with autism spectrum disorder (ASD), affective disorders, multiple sclerosis (MS) and Parkinson’s disease (PD). Non-SCFAs yielded less significantly distinct changes in faecal levels of patients and healthy controls, with the majority of findings were derived from urinary and blood samples. Preclinical studies have implicated different bacterial metabolites with potentially beneficial as well as detrimental mechanisms in brain diseases. Examples include immunomodulation and changes in catecholamine production by histone deacetylase inhibition, anti-inflammatory effects through activity on the aryl hydrocarbon receptor and involvement in protein misfolding. Overall, our findings highlight the existence of altered bacterial metabolites in patients across various brain diseases, as well as potential neuroactive effects by which gut-derived SCFAs, p-cresol, indole derivatives and bacterial amyloids could impact disease development and progression. The findings summarized in this review could lead to further insights into the gut–brain–axis and thus into potential diagnostic, therapeutic or preventive strategies in brain diseases.

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“…By comparing the AD mouse model and wild-type mouse model of different ages, the perturbed a-diversity of microbiota and decreased level of SCFAs were found to be associated with the bacterial amyloid deposition and ultrastructural alteration in the gut (133). In a similar trial by Zheng et al (134) on the content of SCFAs in fecal samples, the AD mouse model showed a significant difference in the levels of propionic acid, isobutyric acid, 3-hydroxy butyric acid, and 3hydroxisopropyl acid as well as the decreased levels of lactic acid, 2-hydroxy butyric acid, 2-hydroxy isobutyric acid, levulinic acid, and valproic acid compared with wild-type mice. In addition, an in vitro study suggested that sodium butyrate could protect the neuron cells from Ab-induced neurotoxic effects (135).…”

Section: Modifications Of Microbial Molecules In Alzheimer's Diseasementioning

confidence: 95%

Implications of Gut Microbiota in Neurodegenerative Diseases

et al. 2022

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The morbidity associated with neurodegenerative diseases (NDs) is increasing, posing a threat to the mental and physical quality of life of humans. The crucial effect of microbiota on brain physiological processes is mediated through a bidirectional interaction, termed as the gut–brain axis (GBA), which is being investigated in studies. Many clinical and laboratory trials have indicated the importance of microbiota in the development of NDs via various microbial molecules that transmit from the gut to the brain across the GBA or nervous system. In this review, we summarize the implications of gut microbiota in ND, which will be beneficial for understanding the etiology and progression of NDs that may in turn help in developing ND interventions and clinical treatments for these diseases.

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Stable isotope labeling combined with liquid chromatography-tandem mass spectrometry for comprehensive analysis of short-chain fatty acids

Cited by 53 publications

References 41 publications

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

The Role of Gut Bacterial Metabolites in Brain Development, Aging and Disease

Implications of Gut Microbiota in Neurodegenerative Diseases

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