Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs

Hood‐Nowotny, Rebecca; Harari, Ally R.; Seth, Rakesh; Wee, Suk‐Ling; Conlong, Des E.; Suckling, D. M.; Woods, Bill; Lebdi-Grissa, Kaouthar; Simmons, Gregory S.; Carpenter, James E.

doi:10.1653/024.099.sp120

Cited by 16 publications

(12 citation statements)

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“…This usefulness is reinforced by the fact that the δ 13 C values of males did not differ from females, neither in flies reared on different larval diets nor in wild flies (Tables 1 and 3). This finding is in line with Hood-Nowotny et al [22] that also found no significant differences in isotope signatures between males and females of the cactus moth. However, it seems that sex differences depend on the species.…”

Section: Discussionsupporting

confidence: 92%

“…As the isotopic signature of an animal reflects the isotopic composition of its food [15, 16], the carbon and nitrogen isotopic signature can be used to track feeding patterns of insects, for instance. Many studies have demonstrated that stable isotopes can be suitable intrinsic markers for mosquitoes [17, 18, 19], tsetse flies [20], moths [21, 22] and fruit flies [23].…”

Section: Introductionmentioning

confidence: 99%

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Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies?

et al. 2018

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The availability of accurate techniques to discriminate between marked laboratory-reared flies and unmarked wild flies captured in monitoring traps is essential for programs that integrate the Sterile Insect Technique (SIT) to manage fruit flies. In this study, the feasibility of using a stable isotope marking technique for the South American fruit fly, Anastrepha fraterculus (Wiedemann), was assessed. Wild flies were collected from apple orchards, which are a target of a SIT project in southern Brazil. To verify if adult flies could be labelled by the stable isotopes from larval diets, larvae were reared on two different C4-based diets and fruits in laboratory. To evaluate the influence of the two most common attractants applied to capture A. fraterculus (grape juice and CeraTrapTM) and the most common preservation method in fruit fly collections (ethanol), laboratory-reared flies were immersed in McPhail traps containing the respective treatments for two periods of time. Samples were analyzed in an elemental analyzer coupled to a Continuous Flow Isotope Ratio Mass Spectrometer (CF-IRMS) at CENA/USP. The δ13C signatures of flies reared on artificial diets differed significantly from the δ13C of flies whose larvae were reared on fruits and from wild flies. In contrast, the δ15N values were less conclusive and the technique could not rely solely on them. In all cases considered, the δ13C and δ15N signatures from males did not differ from females. Despite the alterations caused by the attractants tested and ethanol, laboratory-flies could be distinguished from the wild ones based on δ13C signatures. This is the first comprehensive study to demonstrate that it is possible to distinguish wild A. fraterculus from flies reared on larval diets containing C4 sugar. The first experimentally derived trophic discrimination factors were also obtained for this species. Thus, intrinsic isotope labelling can serve as a backup to conventional dye marking.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies?

et al. 2018

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“…Insects undergo a behaviour-altering chain of processes during mass-rearing in an artificial environment and during handling and irradiation procedures before being released in the target area. The importance of mating compatibility between the released strain and the wild target population cannot be overemphasised and was already assessed with some fruit fly [ 31 ], tsetse fly [ 32 ] and Lepidoptera species [ 33 , 34 ]. Studies with the Mediterranean fruit fly C .…”

Section: Discussionmentioning

confidence: 99%

One for all: Mating compatibility among various populations of olive fruit fly (Diptera: Tephritidae) for application of the sterile insect technique

Ahmad

Haq

Cáceres³

et al. 2018

PLoS ONE

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The olive fruit fly, Bactrocera oleae (Rossi), is the most important insect pest for the cultivation of olives worldwide. Considerable research efforts have been invested in the past decades to develop eradication or suppression tactics for use within an area-wide integrated pest management (AW-IPM) approach that includes a sterile insect technique (SIT) component. One of the major obstacles encountered in the development of SIT for olive fruit fly was the inferior quality of the mass-reared flies, expressed among others evident primarily by sterile males having a different timing of peak mating and a lower mating propensity in comparison with their wild counterparts. In this study we assessed the mating behaviour and mating compatibility of olive fruit flies originating from four countries of the Mediterranean region (Croatia, France, Italy, Spain) in walk-in field cages and post zygotic compatibility (expressed as % egg hatch) under laboratory conditions. Furthermore, we tested the hypothesis whether a hybrid strain (Greece (domesticated)/Israel (wild)) adapted to laboratory rearing conditions showed any mating barriers with all the four “wild” populations. Finally, we examined the effect of colonization on the mating compatibility of the four newly established populations over three consecutive generations. The results showed no pre-zygotic (mating barriers) or post-zygotic isolations (measured by egg hatch%) among the olive fruit fly populations from the four countries tested. Also, there was no evidence of mating barriers between the hybrid strain and the wild populations of the Mediterranean region.

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“…By enriching larval habitats with either 15 N or 13 C, we are elevating this rare form of element well above natural abundance levels which can serve as a label to differentiate the enriched mosquitoes from those that develop in unenriched environments. Stable isotopes have been widely used for identification purposes of different insects such as moths 12 , tsetse flies 13 , fruit flies 14 and mosquitoes [15][16][17] , and studies have shown minimal impacts on their physiology, behavior and ecology 18,19 . Stable isotopic marking of Anopheles gambiae s. l., Culex quinquefasciatus and Aedes spp.…”

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confidence: 99%

Dispersal of female and male Aedes aegypti from discarded container habitats using a stable isotope mark-capture study design in South Texas

Juarez

Garcia-Luna

Chaves

et al. 2020

Sci Rep

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Aedes aegypti is the main vector of arboviral diseases such as dengue, chikungunya and Zika. A key feature for disease transmission modeling and vector control planning is adult mosquito dispersal. We studied Ae aegypti adult dispersal by conducting a mark-capture study of naturally occurring Ae. aegypti from discarded containers found along a canal that divided two residential communities in Donna, texas, USA. Stable isotopes were used to enrich containers with either 13 c or 15 n. Adult mosquitoes were collected outdoors in the yards of households throughout the communities with BG Sentinel 2 traps during a 12-week period. Marked mosquito pools with stable isotopes were used to estimate the mean distance travelled using three different approaches (Net, Strip or Circular) and the probability of detecting an isotopically marked adult at different distances from the larval habitat of origin. We consistently observed, using the three approaches that male (Net: 220 m, Strip: 255 m, Circular: 250 m) Ae. aegypti dispersed further in comparison to gravid (Net: 135 m, Strip: 176 m, Circular: 189 m) and unfed females (Net: 192 m, Strip: 213 m, Circular: 198 m). We also observed that marked male capture probability slightly increased with distance, while, for both unfed and gravid females, such probability decreased with distance. Using a unique study design documenting adult dispersal from natural larval habitat, our results suggest that Ae. aegypti adults disperse longer distances than previously reported. These results may help guide local vector control authorities in their fight against Ae. aegypti and the diseases it transmits, suggesting coverage of 200 m for the use of insecticides and innovative vector control tools.

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Stable Isotope Markers Differentiate between Mass-Reared and Wild Lepidoptera in Sterile Insect Technique Programs

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 16 publications

References 40 publications

Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies?

Can stable isotope markers be used to distinguish wild and mass-reared Anastrepha fraterculus flies?

One for all: Mating compatibility among various populations of olive fruit fly (Diptera: Tephritidae) for application of the sterile insect technique

Dispersal of female and male Aedes aegypti from discarded container habitats using a stable isotope mark-capture study design in South Texas

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