Stable-isotope probing coupled with high-throughput sequencing reveals bacterial taxa capable of degrading aniline at three contaminated sites with contrasting pH

Liu, Huaqing; Lin, Hanzhi; Song, Benru; Sun, Xiaoxu; Xu, Rui; Kong, Tianle; Xu, Fuqing; Li, Baoqin; Sun, Weimin

doi:10.1016/j.scitotenv.2020.144807

Cited by 14 publications

(6 citation statements)

References 44 publications

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“…Thus, the co-occurrence of the above microbial communities confirmed that a "small world" was established in the aniline-spiked microcosm, as the previous studies suggested [30]. In addition, some bacteria only had a high proportion in the heavy fractions at 45 or 69 h. This change was considered to be a natural succession in microbial communities involved in aniline degradation over time [18].…”

Section: Aniline-degrading Bacteria Identified In Aerobic Microcosmsupporting

confidence: 81%

“…Due to small change in the aniline concentration in the sterilization group, we concluded that the degradation in the aniline concentration in the experimental groups was mainly due to microbial degradation [12]. Moreover, the aniline-degradation rate increased with an increasing culture time, which may have been due to the gradual accumulation in functional bacteria [18]. Therefore, after the degradation in 13 C-aniline, a clear peak appeared in the heavy DNA fractions during isopycnic density gradient centrifugation.…”

Section: Degradation In Aniline and Uptake Of 13 C By The Microorganismsmentioning

confidence: 85%

“…After adding 10 mM 13 C-aniline or 12 C-aniline to the cultures, they were sealed with a breathable membrane and incubated at room temperature in the dark with shaking at 120 rpm. The concentration in aniline was determined in triplicate using an Agilent 1100 HPLC (Agilent, Santa Clara, CA, USA) with 0.1 mL supernatant liquid taken from the degradation system at intervals [18]. Then the degradation rate of the aniline in the microcosms was calculated [37].…”

Section: Soil Microcosms Incubationsmentioning

confidence: 99%

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Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Li,

Ghani,

Sun

et al. 2024

Catalysts

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Aniline, a vital component in various chemical industries, is known to be a hazardous persistent organic pollutant that can cause environmental pollution through its manufacturing, processing, and transportation. In this study, the microcosms were established using sediment with a history of aniline pollution as an inoculum to analyze the aniline biodegradation under aerobic conditions through stable isotope probing (SIP) and isopycnic density gradient centrifugation technology. During the degradation assay, aniline that was 13C-labeled in all six carbons was utilized to determine the phylogenetic identity of the aniline-degrading bacterial taxa that incorporate 13C into their DNA. The results revealed that aniline was completely degraded in the microcosm after 45 and 69 h respectively. The bacteria affiliated with Acinetobacter (up to 34.6 ± 6.0%), Zoogloea (up to 15.8 ± 2.2%), Comamonas (up to 2.6 ± 0.1%), and Hydrogenophaga (up to 5.1 ± 0.6%) genera, which are known to degrade aniline, were enriched in the heavy fractions (the DNA buoyant density was 1.74 mg L−1) of the 13C-aniline treatments. Moreover, some rarely reported aniline-degrading bacteria, such as Prosthecobacter (up to 16.0 ± 1.6%) and Curvibacter (up to 3.0 ± 1.6%), were found in the DNA-SIP experiment. Gene families affiliated with atd, tdn, and dan were speculated to be key genes for aniline degradation based on the abundance in functional genes and diversity in different treatments as estimated using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States version 2 (PICRUSt2) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). This study revealed the functional bacteria and possible degradation genes for aniline degradation in simulated polluted environments through SIP. These findings suggest that important degrading bacteria for the transformation of aniline and potential degradation pathways may be useful in the effective application of bioremediation technologies to remediate aniline-contaminated sites.

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Section: Aniline-degrading Bacteria Identified In Aerobic Microcosmsupporting

confidence: 81%

Section: Degradation In Aniline and Uptake Of 13 C By The Microorganismsmentioning

confidence: 85%

Section: Soil Microcosms Incubationsmentioning

confidence: 99%

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Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Li,

Ghani,

Sun

et al. 2024

Catalysts

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“…Tepidisphaerales is an aniline-degrading bacteria in an acidic environment [56]. We therefore speculate that the relative abundance of some beneficial microorganisms increased under the effect of seed coating, exerting a regulatory function in the soil ecology and plant health.…”

Section: Discussionmentioning

confidence: 87%

Microbiological and Mechanism Analysis of Novel Wheat Seed Coating Agents-Induced Growth Promotion of Wheat Seedlings

Chen,

Wang,

et al. 2024

Agronomy

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TFC (10% thifluzamide–fludioxonil–clothianidin) is a novel wheat seed-coating agent. In the field, we confirmed that 10% TFC plays a positive role in preventing soil-borne diseases and promoting wheat seedling growth. However, its effects on rhizosphere microecology and the underlying molecular mechanism are not fully understood. Field trials revealed a positive effect on the biomass, plant height, and root length of wheat sharp eyespots in a Yingshang field, with 95.3% control efficiency. The effects of 10% TFC on the rhizosphere soil microbiome of young wheat plants were evaluated using high throughput sequencing technology. The results demonstrated that seed-coating agents significantly changed bacterial and fungal communities, and reduced the number of bacteria but increased the number of fungi. Sequence analysis revealed that the abundance of Proteobacteria, Actinobacteria, and Patescibacteria in bacteria and Ascomycota, Mortierellomycota, and Basidiomycota in fungi were significantly enriched, which have been reported as being beneficial for plant growth and pathogen resistance. In contrast, the abundance of Mucoromycota in fungi was reduced, and most of the related genera identified were pathogenic to plants. In this study, 15-day-old wheat plant tissues treated with 10% TFC were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of 10% TFC on seedling growth. The comparative analysis of Triticum aestivum L. libraries identified 8286 differentially expressed genes (DEGs), of which 2290 and 5996 genes were up- and downregulated in seedling growth in the presence of 10% TFC, respectively. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses were performed for up- and downregulated DEGs separately, showing that these DEGs were enriched for terms related to the phenylpropanoid biosynthesis pathway, the protein products of which promote cell differentiation and seedling growth. This research provides comprehensive insights into its effects on wheat seedling growth and the rhizosphere microecology of seed coatings and provides important insights into their regulation and into understanding the potential benefits of seed coatings in disease management and plant growth promotion.

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“…Therefore, they can be absorbed by the human body through skin and tissue contact and accumulated in vivo, threatening human health, and have been included in a list of major toxicants [11,12]. At present, aniline derivatives in water could generally be removed or separated by catalytic degradation and adsorption of materials [13][14][15][16][17], but they are difficult to remove from soil and rock surfaces.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cucurbit[7]uril on Adsorption of Aniline Derivatives at Quartz

et al. 2022

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The adsorption behavior of small molecules at solid–liquid interfaces have become an important research topic in recent years. For example, small molecules of aniline pollutants will adsorb on solid surfaces with a massive discharge of industrial wastewater and are difficult to separate. Therefore, their adsorption and desorption on solid surfaces have become an important scientific problem. In this study, the interactions of cucurbit[7]uril (Q[7]) with 4,4′-diaminodiphenylmethane (MDA) and benzidine (AN) are studied using 1H NMR, UV-Vis spectrometry and fluorescence spectroscopy. The results show that Q[7] forms an inclusion complex with MDA and AN. According to the results of Quartz Crystal Microbalance with Dissipation (QCM-D), MDA adsorbs onto a quartz surface and forms a viscous adsorption layer on it. The AN adsorbs on the quartz surface and forms a rigid adsorption film on it. Q[7] can reduce the adsorption of MDA on the quartz surface and increases the adsorption of AN on it. Through the dynamic adsorption experiments, we find that Q[7] has a desorption effect on MDA molecules adsorbed on the quartz surface. An Atomic Force Microscope (AFM) is used to measure the morphological changes in the adsorption film before and after Q[7] makes contact with the quartz surface, and it proves that Q[7] has a desorption effect on MDA molecules adsorbed on the surface.

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Stable-isotope probing coupled with high-throughput sequencing reveals bacterial taxa capable of degrading aniline at three contaminated sites with contrasting pH

Cited by 14 publications

References 44 publications

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Identification of Aniline-Degrading Bacteria Using Stable Isotope Probing Technology and Prediction of Functional Genes in Aerobic Microcosms

Microbiological and Mechanism Analysis of Novel Wheat Seed Coating Agents-Induced Growth Promotion of Wheat Seedlings

Effect of Cucurbit[7]uril on Adsorption of Aniline Derivatives at Quartz

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