2024
DOI: 10.1101/2024.01.24.576656
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Stable Isotope Probing-nanoFTIR for Quantitation of Cellular Metabolism and Observation of Growth-dependent Spectral Features

David J. Burr,
Janina Drauschke,
Katerina Kanevche
et al.

Abstract: This study utilizes nanoscale Fourier transform infrared spectroscopy (nanoFTIR) to perform stable isotope probing (SIP) on individual bacteria cells cultured in the presence of13C-labelled glucose. SIP-nanoFTIR simultaneously quantifies single-cell metabolism through IR spectroscopy and acquires cellular morphological information via atomic force microscopy. The redshift of the amide I peak corresponds to the isotopic enrichment of newly synthesized proteins. These observations of single-cell translational ac… Show more

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