Stable isotope probing of acetate fed anaerobic batch incubations shows a partial resistance of acetoclastic methanogenesis catalyzed by Methanosarcina to sudden increase of ammonia level

Hao, Liping; Lü, Fan; Mazéas, Laurent; Quéméner, Elie Desmond-Le; Madigou, Céline; Guenne, Angéline; Shao, Liming; Bouchez, Théodore; He, Pinjing

doi:10.1016/j.watres.2014.11.010

Cited by 80 publications

(37 citation statements)

References 29 publications

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“…However, the increasing addition of ammonium chloride did not influence the carbon isotope composition of the biogas, and no major shift in the methanogenic pathways can be assumed. This is in agreement with the results of previous studies where high TAN concentrations were adjusted by ammonium chloride, which had no major impact on the relative contribution of aceticlastic methanogenic activity , . The disagreement found between the chicken manure and the ammonium chloride results warrants that ammonium chloride or other ammonium salts are not appropriate to study ammonia inhibition during manure digestion.…”

Section: Resultsmentioning

confidence: 99%

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Jiang

Liebetrau

et al. 2018

Chem Eng & Technol

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Anaerobic digestion of poultry manure poses many challenges due to ammonia inhibition by nitrogen‐containing compounds. Here, the effect of NH4Cl or urea‐induced inhibition was investigated in bioreactors fed with maize silage or cellulose and compared to previous experiments with chicken manure as ammonia source. The hydrogen and carbon stable isotope compositions of biogas samples were evaluated to assess the shifts in predominant methanogenic pathways during increasing ammonia inhibition. Due to the higher free ammonia concentrations, stronger inhibition was observed in the urea added reactors compared to the NH4Cl treated ones. Collectively, the results suggested that ammonium salts are not really appropriate to investigate ammonia inhibition during anaerobic digestion of manure.

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Section: Resultsmentioning

confidence: 99%

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Jiang

Liebetrau

et al. 2018

Chem Eng & Technol

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“…However, although Methanosarcina spp. are reported as highly tolerant to others toxicants, such as ammonia and salts (De Vrieze et al ., ; Hao et al ., ), M. mazei was more vulnerable to palmitate than M. concilii . Palmitate concentrations of 2 mmol L −1 caused a 64% decrease of methane production by M. mazei , while for the same concentration the methanogenic inhibition of M. concilii was only 11% (Table ).…”

Section: Resultsmentioning

confidence: 99%

Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei

Silva

Salvador

Cavaleiro

et al. 2016

Microbial Biotechnology

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SummaryLong‐chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures of Methanosaeta concilii and Methanosarcina mazei was assessed by comparing methane production rates from acetate before and after LCFA addition. For both methanogens, a sharp decrease in methane production (> 50%) was observed at 0.5 mmol L−1 oleate, and no methane was formed at concentrations higher than 2 mmol L−1 oleate. Palmitate was less inhibitory than oleate, and M. concilii was more tolerant to palmitate than M. mazei, with 2 mmol L−1 palmitate causing 11% and 64% methanogenic inhibition respectively. This study indicates that M. concilii and M. mazei tolerate LCFA concentrations similar to those previously described for hydrogenotrophic methanogens. In particular, the robustness of M. concilii might contribute to the observed prevalence of Methanosaeta species in anaerobic bioreactors used to treat LCFA‐rich wastewater.

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“…The primers 349F and 806R can effectively detected archaeal rDNA (Takai and Horikoshi 2000), and it was widely used in many studies to target archaeal communities by highthroughput 16S rRNA gene pyrosequencing (Cortés-Lorenzo et al 2014;Frank et al 2013;Sato et al 2013;Hao et al 2015;Rago et al 2015). But, among them, one study showed that archaeal sequences comprised only a low proportion (1-49 %) when this primer was used (Sato et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…But, among them, one study showed that archaeal sequences comprised only a low proportion (1-49 %) when this primer was used (Sato et al 2013). Another research also mentioned that OTUs identified as bacteria and eukarya among sequences obtained by using archaea-specific primers were discarded in data analysis part (Hao et al 2015). Such reports indicated the existence of non-archaeal sequences, though their abundance not shown.…”

Section: Discussionmentioning

confidence: 99%

Archaeal communities in the sediments of different mangrove stands at Dongzhaigang, China

Guan

Chen

et al. 2016

J Soils Sediments

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Purpose Knowledge of archaeal communities is essential for understanding of the mechanism of carbon and nitrogen cycle in the mangrove sediment ecosystem. Presently, little is known about archaeal communities in the Dongzhaigang mangrove sediments. This study aimed to characterize the archaeal communities in sediments of different mangrove stands and to find out the correlations between archaeal communities and the environmental factors of sediments.Materials and methods Sediment samples were collected from the Dongzhaigang mangrove forest for analysis of soil properties and archaeal communities, by national standard methods and Illumina Miseq archaeal 16S ribosomal RNA (rRNA) gene sequencing, respectively. Results and discussion The archaeal community in the Dongzhaigang mangrove forest was constituted by some phyla from BTACK^and BDPANN^supergroups, and dominated by Euryarchaeota. Among sediments of the four mangroves in Dongzhaigang, principal coordinates analysis (PCoA) scatter plot showed a trend of difference in the archaeal community structure in the Bruguiera gymnoihiza and Kandelia candel stands from that in the Laguncularia racemosa and Sonneratia apetala stands. The abundance of the order Methanosarcinales was the highest in the sediments of K. candel mangroves, whereas the order of Methanobacteriales dominated in B. gymnoihiza sediments. The highest richness and diversity values of Archaea occurred in K. candel sediments, while the lowest in B. gymnoihiza. Pearson correlation showed the significant relationships between sediment properties and some dominant genera, with a positive and significant correlation between sediment properties and genus Methanobacterium, coinciding with the maximum values of sediment properties and abundance of Methanobacterium in the sediment of B. gymnoihiza. Such results indicated that the difference of archaeal community structure among mangrove sediments may be caused by the different sediment characteristics. Methanogenic communities in the Dongzhaigang mangrove forest sediments were, at the order level, constituted by Methanobacteriales, Methanomicrobiales, Methanosarcinales, and Methanomassiliicoccales. Conclusions The investigation indicated that the Dongzhaigang mangrove sediment ecosystems support (2016( ) 16:1995( -2004( DOI 10.1007( /s11368-016-1427 diverse archaeal communities and methanogenic communities, and that there was a general trend of difference in the archaeal community structure in the B. gymnoihiza and K. candel mangrove sediments from that in the L. racemosa and S. apetala sediments. Such difference may be caused by the difference in sediment characteristics.

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Stable isotope probing of acetate fed anaerobic batch incubations shows a partial resistance of acetoclastic methanogenesis catalyzed by Methanosarcina to sudden increase of ammonia level

Cited by 80 publications

References 29 publications

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Ammonium Chloride vs Urea‐Induced Ammonia Inhibition of the Biogas Process Assessed by Stable Isotope Analysis

Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei

Archaeal communities in the sediments of different mangrove stands at Dongzhaigang, China

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