2021
DOI: 10.3389/fcvm.2021.734364
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Stable Isotopes for Tracing Cardiac Metabolism in Diseases

Abstract: Although metabolic remodeling during cardiovascular diseases has been well-recognized for decades, the recent development of analytical platforms and mathematical tools has driven the emergence of assessing cardiac metabolism using tracers. Metabolism is a critical component of cellular functions and adaptation to stress. The pathogenesis of cardiovascular disease involves metabolic adaptation to maintain cardiac contractile function even in advanced disease stages. Stable-isotope tracer measurements are a pow… Show more

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Cited by 11 publications
(6 citation statements)
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References 109 publications
(121 reference statements)
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“…While traditional metabolic measurement techniques focus on net substrate concentrations at specific time points, isotope tracing can infer metabolic flux over a defined period, measuring substrate flow through a pathway per unit of time. 116 This technology, proven effective in various models and organ systems, 11 , 12 , 117 , 118 is crucial for characterizing the intricate chemical changes contributing to secondary brain injury (Figure 1 , Tables 2 and 3 ).…”
Section: Isotope Tracingmentioning
confidence: 99%
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“…While traditional metabolic measurement techniques focus on net substrate concentrations at specific time points, isotope tracing can infer metabolic flux over a defined period, measuring substrate flow through a pathway per unit of time. 116 This technology, proven effective in various models and organ systems, 11 , 12 , 117 , 118 is crucial for characterizing the intricate chemical changes contributing to secondary brain injury (Figure 1 , Tables 2 and 3 ).…”
Section: Isotope Tracingmentioning
confidence: 99%
“…NMR and LC/GC–MS are extensively used analytic techniques in isotope tracing studies in the current literature. 11 , 12 , 13 , 33 , 117 , 118 , 119 , 120 These established techniques provide detailed data on numerous substrates and metabolites in a sample. Recent computing advancements facilitate cross‐referencing metabolite readouts to libraries, aiding in identifying unexpected pathway alterations.…”
Section: Isotope Tracingmentioning
confidence: 99%
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“…Additional information on the flux through an enzymatic reaction or network is required to answer this question. Flux analysis encompasses a set of different tools to answer such questions, including tracer-based metabolomics, 12,126,127 measurements of enzymatic activities, or computational modeling [128][129][130] (Figure 3B). The most important tools for flux analysis are tracer-based studies, including stable-isotope tracers of common nutrients or radioactive probes.…”
Section: Metabolic Tracing and Flux Analysismentioning
confidence: 99%
“…Given the complexities of extracellular purine metabolism, future studies on the dynamics of this pathway might involve the application of isotopically labelled tracers combined with NMR and mass spectrometry, techniques that were successfully applied in the past to metabolic pathways in various organs [ 36 ]. Challenges persist as to the accurate estimation of substrate concentrations and flux rates from stable-isotope tracers.…”
Section: What Comes Next?mentioning
confidence: 99%