Stable Isotopic Tracing of Nanomaterials<i>In Vivo</i>

Zhao, Yuliang; Chang, Xueling

doi:10.1002/9783527689125.ch3

Cited by 2 publications

(3 citation statements)

References 112 publications

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“…Stable isotopic tracing has shown great promise in investigating the environmental risks of CNMs to address questions regarding their uptake and accumulation in vivo . Stable isotope ( 13 C) labeling of the carbon skeleton is a well-established approach for quantifying CNMs in biological samples by analyzing the 13 C/ 12 C ratio with isotope ratio mass spectrometry (IRMS) .…”

Section: Introductionmentioning

confidence: 99%

“…Stable isotopic tracing has shown great promise in investigating the environmental risks of CNMs to address questions regarding their uptake and accumulation in vivo. 27 Stable isotope ( 13 C) labeling of the carbon skeleton is a wellestablished approach for quantifying CNMs in biological samples by analyzing the 13 C/ 12 C ratio with isotope ratio mass spectrometry (IRMS). 28 The 13 C-labeling of the carbon skeleton does not influence the stability or intrinsic structure of CNMs and allows the properties of CNMs to be traced in biological systems without producing radioactive wastes.…”

Section: ■ Introductionmentioning

confidence: 99%

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Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Feng

et al. 2023

Environ. Sci. Technol.

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While carbon dots (CDs) have the potential to support the agricultural revolution, it remains obscure about their environmental fate and bioavailability by plants. Fungal laccase-mediated biotransformation of carbon nanomaterials has received little attention despite its known capacity to eliminate recalcitrant contaminants. Herein, we presented the initial investigation into the transformation of CDs by fungal laccase. The degradation rates of CDs were determined to be firstorder in both substrate and enzyme. Computational docking studies showed that CDs preferentially bonded to the pocket of laccase on the basal plane rather than the edge through hydrogen bonds and hydrophobic interactions. Electrospray ionization-Fourier transform-ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS) and other characterizations revealed that the phenolic/amino lignins and tannins portions in CDs are susceptible to laccase transformation, resulting in graphitic structure damage and smaller-sized fragments. By using the 13 C stable isotope labeling technique, we quantified the uptake and translocation of 13 C-CDs by mung bean plants. 13 C-CDs (10 mg L −1 ) accumulated in the root, stem, and leaf were estimated to be 291, 239, and 152 μg g −1 at day 5. We also evidenced that laccase treatment alters the particle size and surface chemistry of CDs, which could facilitate the uptake of CDs by plants and reduce their nanotoxicity to plants.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Feng

et al. 2023

Environ. Sci. Technol.

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“…Before investigating the exposure route of fullerene, it is necessary to establish an effective quantification method for complex environmental or biological systems . Because of the high background level of carbon in the environment and organisms, it is extremely important to develop a method for high-precision quantification of ecosystem carbon nanomaterials. , Among the existing analytical methods, isotope labeling methods, including radioactive and stable isotope labeling, comprise a very powerful tool to trace exogenous carbon elements and quantify carbon nanomaterials in organisms. − Radioactive labeling has been widely applied in the evaluation of bioaccumulation of carbon nanomaterials in vivo ; however, there are significant drawbacks associated with this method, such as the strict conditions required for use of radioactivity, the generation of radioactive wastes, and the shorter half-lives of some radioactive elements. To overcome these drawbacks, stable isotope labeling has been successfully applied to quantify and trace metal/metal oxides and carbon nanoparticles in vivo.…”

Section: Introductionmentioning

confidence: 99%

Uptake and Transfer of ¹³C-Fullerenols from Scenedesmus obliquus to Daphnia magna in an Aquatic Environment

Wang

Chang

Shi

et al. 2018

Environ. Sci. Technol.

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Fullerenol, a water-soluble polyhydroxylated fullerene nanomaterial, enters aquatic organisms and ecosystems through different ingestion exposures and may pose environmental risks. The study of their uptake routes and transfer in aquatic systems is scarce. Herein, we quantitatively investigated the aquatic uptake and transfer of 13 C-fullerenols from Scenedesmus obliquus to Daphnia magna using 13 C-skeletonlabeling techniques. The bioaccumulation and depuration of fullerenol in Daphnia magna increased with exposure doses and time, reaching steady state within 16 h in aqueous and feeding-affected aqueous routes. The capacity of Daphnia magna to ingest fullerenol via the aqueous route was much higher than that via the dietary route. From the aqueous to feeding-affected aqueous, the kinetic analysis demonstrated the bioaccumulation factors decreases, which revealed that algae suppressed Daphnia magna uptake of fullerenols. The aqueous route was the primary fullerenols ingestion pathway for Daphnia magna. Kinetic analysis of the accumulation and transfer in Daphnia magna via the dietary route indicated low transfer efficiency of fullerenol along the Scenedesmus obliquus-Daphnia magna food chain. Using stable isotope labeling techniques, these quantitative data revealed that carbon nanomaterials underwent complex aquatic accumulation and transfer from primary producers to secondary consumers and algae inhibited their transfer in food chains.

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Stable Isotopic Tracing of NanomaterialsIn Vivo

Cited by 2 publications

References 112 publications

Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Uptake and Transfer of ¹³C-Fullerenols from Scenedesmus obliquus to Daphnia magna in an Aquatic Environment

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Stable Isotopic Tracing of NanomaterialsIn Vivo

Cited by 2 publications

References 112 publications

Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Mechanistic Insights into the Biodegradation of Carbon Dots by Fungal Laccase

Uptake and Transfer of 13C-Fullerenols from Scenedesmus obliquus to Daphnia magna in an Aquatic Environment

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Uptake and Transfer of ¹³C-Fullerenols from Scenedesmus obliquus to Daphnia magna in an Aquatic Environment