Stable low molecular weight RNA profiling showed variations within Sinorhizobium meliloti and Sinorhizobium medicae nodulating different legumes from the alfalfa cross-inoculation group

Abstract

Four different low molecular weight (LMW) RNA profiles, designated I-IV, among 179 isolates from Medicago, Melilotus and Trigonella species growing in a field site in Northern Spain were identified. From sequence analysis of the 16S rRNA, atpD and recA genes as well as DNA-DNA hybridization analysis with representatives of each LMW RNA profile it was evident that isolates with LMW RNA profiles I and II belonged to Sinorhizobium meliloti and those displaying profiles III and IV to Sinorhizobium medicae. Therefore, two distinct LMW RNA electrophoretic mobility profiles were found within each of these two species. Collectively, LMW RNA profiles I and II (identified as S. meliloti) were predominant in Melilotus alba, Melilotus officinalis and Medicago sativa. Profiles III and IV (identified as S. medicae) were predominant in Melilotus parviflora, Medicago sphaerocarpa, Medicago lupulina and Trigonella foenum-graecum. All the four LMW RNA profiles were identified among isolates from Trigonella monspelliaca nodules. These results revealed a different specificity by the hosts of the alfalfa cross-inoculation group towards the two bacterial species found in this study.

MALDI-TOF Mass Spectrometry Is a Fast and Reliable Platform for Identification and Ecological Studies of Species from Family Rhizobiaceae

“…In genus Ensifer , E. meliloti RPA13 and RTM17 and E. medicae RMP01, RMP05, RPA08, RPA11 and RPA20 belong to the same biovar (meliloti) [49]. However, in agreement with rrs and housekeeping gene analyses (figures 2, 3 and 4), the strains of these both species were clearly distinguished by MALDI-TOF MS (Table 2F).…”

Diversity of rhizobia and interactions among the host legumes and rhizobial genotypes in an agricultural-forestry ecosystem

“…Only S. meliloti was isolated from nodules of Medicago lupulina in the present study, while this legume mainly nodulated with S. medicae in Northern Spain (Del Villar et al 2008) and with diverse populations related to Rhizobium spp., S. fredii and S. meliloti in Qinghai-Tibet plateau (Kan et al 2007;Hou et al 2008). Similar situations were also found in rhizobia associated with G. max, G. soja, K. stipulacea P. vulgaris, and L. bicolor (Table 3).…”

Rhizobia from Lanzarote, the Canary Islands, That Nodulate Phaseolus vulgaris Have Characteristics in Common with Sinorhizobium meliloti Isolates from Mainland Spain

“…Isolation and characterization of rhizobia from Medicago, Melilotus, and Trigonella spp. originating from León, Spain, have been described by del Villar et al (5).…”

“…The class 1 and class 2 tRNA patterns of RTM17 and the bean isolate were identical, while the two tRNA patterns produced in the lanes with the type strains for S. fredii, S. medicae, and S. meliloti were dissimilar. Therefore, the bean isolates from Lanzarote were placed in LMW RNA group II of S. meliloti as described recently by del Villar et al (5).…”