Stable silencing of TIPE2 reduced the Poly I:C-induced apoptosis in THP-1 cells

Jiang, Jieshu; Wang, Shanshan; Jingjing, Fang; Xu, Yi; Li, Tong; Ye, Xiaolei; Weiyuan, Zhou

doi:10.3892/mmr.2017.7364

Cited by 5 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The result was consistent with down-regulated expression of TIPE2 in M1 macrophages that can release neutrophilic inflammation factors and aggravate neutrophilic inflammation in asthma ( 3 ). TIPE2 was shown to inhibit macrophage apoptosis and M1 phenotype polarization in vitro by decreasing the levels of monocyte chemoattractant protein (MCP)-1, TNF-α, IL-6, IL-1β, and IL-12 ( 19 , 37 , 38 ). TIPE2 may inhibit neutrophilic inflammation in asthma.…”

Section: Discussionmentioning

confidence: 99%

TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage–Related Neutrophilic Inflammation in Asthma

Shi

Hao

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

PurposeAlthough recent studies have highlighted the link of TIPE2 and asthma airway inflammation, its roles and molecular mechanisms in different asthma inflammatory phenotypes remain largely unknown. We evaluated sputum TIPE2 expression level and its correlation with different asthma phenotypes. Additionally, we explored the roles and mechanism of TIPE2 in M1 polarization of macrophages.MethodsA total of 102 asthma patients who underwent sputum induction were enrolled to evaluate the expression level of TIPE2 and its association with different asthma phenotypes. To explore the roles and mechanism of TIPE2 in M1 polarization of macrophages, THP-1 monocytes stimulated with phorbol-12-myristate-13-acetate, were used as a model of undifferentiated (M0) macrophages, and M0 macrophages were treated with lipopolysaccharide to induce M1 macrophages.ResultsThe sputum TIPE2 level was significantly lower in patients with neutrophilic asthma (NA) and higher in patients with eosinophilic asthma (EA) compared with patients with paucigranulocytic asthma. The levels of IL-1β, TNF-α and IL-6 were highest in NA compared with other groups. TIPE2 levels in sputum negatively correlated with IL-1β and TNF-α levels but positively correlated with IL-4, IL-5, IL-13, and IL-10 levels (P < 0.05). In vitro, TIPE2 enhanced Nrf2/HO-1 pathway activation in macrophages and inhibited LPS-induced M1 macrophage differentiation and related cytokine release. Further analysis showed that the Nrf2 inhibitor ML385 weakened TIPE2-induced activation of the Nrf2/HO-1 pathway, as well as TIPE2-induced suppression in M1 polarization of macrophage and inflammatory cytokines secretion.ConclusionsTIPE2 expression level was highly down-regulated in NA and was negatively correlated with inflammatory factors (IL-1β and TNF-α). Aberrant expression of TIPE2 may target the Nrf2/HO-1 pathway to inhibit M1 macrophage–related neutrophilic inflammation in asthma.

show abstract

Section: Discussionmentioning

confidence: 99%

TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage–Related Neutrophilic Inflammation in Asthma

Shi

Hao

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Certain clinical studies have observed that, during the development of GC, the expression profiles of specific biomolecules change. Such changes in expression may serve a key role in tumor progression, including the processes of cell proliferation (٦,١٠,١٨,٢٢,23), motility ( 24 ), adhesion ( 25 ), apoptosis and tissue inflammation. As an inflammatory inhibitor, TIPE2 not only induces cell death, but also inhibits Ras-induced tumor formation, providing a molecular bridge from inflammation to cancer.…”

Section: Discussionmentioning

confidence: 99%

TIPE2 inhibits GC via regulation of cell proliferation, apoptosis and inflammation

et al. 2018

View full text Add to dashboard Cite

Gastric cancer (GC), a type of gastric mucosal epithelium disease caused by common malignant tumors, has become a major threat to human health and survival. Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2) is a negative immune regulatory factor that is selectively expressed in immune organs, immune cells and various epithelial cells and serves an important role in the maintenance of human physiological immune homeostasis. In our preliminary study, we found that the expression of TIPE2 was downregulated or absent in GC tissues compared with normal gastric mucosa tissues, indicating that TIPE2 may play a significant role in the development of GC. To clarify the role of TIPE2 in the progression of human GC and to elucidate the underlying mechanism, the association between TIPE2 and phosphatidylinositol 3-kinase (PI3K)/AKT, the cell cycle, the caspase-related apoptosis pathway and the NF-κB signaling pathway were investigated through western blot and flow cytometric analysis. It was determined that TIPE2 inhibited GC cell proliferation mainly by reducing the expression of phosphorylated AKT and ERK, which caused subsequent inhibition of the PI3K-AKT and Ras-Raf-MEK-ERK1/2 signaling pathways. Additionally, we investigated the relationship between TIPE2 and GC and discovered that TIPE2 inhibited tumor progression via growth, apoptosis and inflammatory pathways. The results of the present study provided a theoretical basis for the development and application of TIPE2 as an antitumor agent.

show abstract

“…45,46 In TIPE2-deficient myeloid cells, polyinosinic-polycytidylic acid (Poly(I:C))mediated dsRNA signaling pathway was overactive, while Poly(I:C) was capable of downregulating TIPE2 expression mediated by downstream cytokines. 47 Furthermore, TIPE2 was found to regulate Rac1-signal transducer and activator of transcription 3 (STAT3) and ERK1/2 signaling pathways and downregulate the expression levels of cyclin D1 and cyclin D3 in mice model of injury-induced restenosis disease 48 (Figure 2).…”

Section: Downstream Effect Factors Of Tipe2mentioning

confidence: 99%

<p>Regulatory Roles of Tumor Necrosis Factor-α-Induced Protein 8 Like-Protein 2 in Inflammation, Immunity and Cancers: A Review</p>

Gu¹,

Chen²,

Sun³

et al. 2020

CMAR

View full text Add to dashboard Cite

Tumor necrosis factor-alpha (TNF-α)-induced protein 8 (TNFAIP8/TIPE) family, including TNFAIP8 (TIPE), TNFAIP8 like-protein 1 (TNFAIP8L1/TIPE1), TNFAIP8 like-protein 2 (TNFAIP8L2/TIPE2), and TNFAIP8 like-protein 3 (TNFAIP8L3/TIPE3), plays a vital role in regulating inflammatory responses, immune homeostasis, and cancer development. Over the last decade, studies have shown that TIPE2 protein is differentially expressed in diverse cells and tissues. The dysregulation of TIPE2 protein can lead to dysregulation of inflammatory responses and immune homeostasis, and change the basic characteristics of cancers. In consideration of the immeasurable values of TIPE2 in diagnosis, treatment, and prognosis of various human diseases, this review will focus on the expression pattern, structure, and regulatory roles of TIPE2 in inflammation, immunity, and cancers.

show abstract

Stable silencing of TIPE2 reduced the Poly I:C-induced apoptosis in THP-1 cells

Cited by 5 publications

References 30 publications

TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage–Related Neutrophilic Inflammation in Asthma

TIPE2 May Target the Nrf2/HO-1 Pathway to Inhibit M1 Macrophage–Related Neutrophilic Inflammation in Asthma

TIPE2 inhibits GC via regulation of cell proliferation, apoptosis and inflammation

<p>Regulatory Roles of Tumor Necrosis Factor-α-Induced Protein 8 Like-Protein 2 in Inflammation, Immunity and Cancers: A Review</p>

Contact Info

Product

Resources

About