Stable, small, specific, low-valency quantum dots for single-molecule imaging

Lee, Jungmin; Feng, Xinyi; Chen, Ou; Bawendi, Moungi G.; Huang, Jun

doi:10.1039/c7nr08673c

Cited by 24 publications

(23 citation statements)

References 55 publications

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“…In parallel, synthetic efforts have been under way to reduce QD size; the primary focus is on replacing the bulky amphiphilic polymer shell with low molecular weight polymers [71] or displacing the native hydrophobic ligands with mono- and bidentate PEGylated alkanethiols [67,72]. Another alternative is the use of imidazole-based block copolymers [73]. Recently, Farlow et al utilized the affinity of phosphorothioate oligonucleotides for the semiconductor surface to prepare monovalent DNA-functionalized QDs [74].…”

Section: Challenges and Prospectsmentioning

confidence: 99%

“…Recently, Farlow et al utilized the affinity of phosphorothioate oligonucleotides for the semiconductor surface to prepare monovalent DNA-functionalized QDs [74]. However, the generation of compact QDs typically requires stripping of native organic ligands which renders compact QDs prone to aggregation and negatively affects optical properties [71,73]. Also, smaller size as a rule results in increased nonspecific binding.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

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Single quantum dot tracking illuminates neuroscience at the nanoscale

Kovtun

Tomlinson

Bailey

et al. 2018

Chemical Physics Letters

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The use of nanometer-sized semiconductor crystals, known as quantum dots, allows us to directly observe individual biomolecular transactions through a fluorescence microscope. Here, we review the evolution of single quantum dot tracking over the past two decades, highlight key biophysical discoveries facilitated by quantum dots, briefly discuss biochemical and optical implementation strategies for a single quantum dot tracking experiment, and report recent accomplishments of our group at the interface of molecular neuroscience and nanoscience.

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Section: Challenges and Prospectsmentioning

confidence: 99%

Section: Challenges and Prospectsmentioning

confidence: 99%

Single quantum dot tracking illuminates neuroscience at the nanoscale

Kovtun

Tomlinson

Bailey

et al. 2018

Chemical Physics Letters

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“…These limitations can be addressed by incorporating fluorescent inorganic nanoparticles, such as quantum dots (QDs), into VLNPs (16)(17)(18). Compared to conventional fluorophores, QDs exhibit enhanced photophysical properties that result in improved sensitivity for biosensing and bioimaging applications (13,(19)(20)(21). While initial concerns regarding toxicity and general biological stability have limited the broad adoption of QDs in the biomedical field, a robust body of work has emerged that shows excellent stability and specificity of biological probes incorporating QDs (22)(23)(24)(25)(26)(27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

A Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Dysregulation of the Neurovascular Unit Relevant to Brain Inflammation

Chiang

Stout

Yanchik-Slade

et al. 2022

Preprint

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Despite limited evidence for competent infection and viral replication of SARS-CoV-2 in the central nervous system (CNS), neurologic dysfunction is a common post-acute medical condition reported in “recovered” COVID-19 patients. To identify a potential noninfectious route for SARS-CoV-2-mediated neurological damage, we constructed colloidal nanocrystal quantum dots linked to micelles decorated with spike protein (COVID-QDs) as a biomimetic to interrogate how blood-brain barrier (BBB) dysregulation may subsequently induce neuroinflammation in the absence of infection. In transwell co-culture of endothelial bEnd.3 monolayers and primary neuroglia, we exposed only the bEnd.3 monolayers to COVID-QDs and examined by fluorescence microscopy whether such treatment led to (i) increased inflammation and leakage across the bEnd.3 monolayers, (ii) permeability of the COVID-QDs across the monolayers, and (iii) induction of neuroinflammation in neuroglial cultures. The results of our study provide evidence of neuroinflammatory hallmarks in cultured neurons and astrocytes without direct exposure to SARS-CoV-2-like nanoparticles. Additionally, we found that pre-treatment of our co-cultures with a small-molecule, broad-spectrum inhibitor of mixed lineage and leucine rich repeat kinases led to reversal of the observed dysregulation in endothelial monolayers and resulted in neuroglial protection. The results reported here may serve to guide future studies into the potential mechanisms by which SARS-CoV-2 mediates neurologic dysfunction.

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“…Along with their extreme brightness and resistance to photobleaching, a color-size dependent profile with distinct broad absorbance, and narrow emission curves, they have been used in live cell imaging over extended periods of time, which is an advantage for the analysis of multiple targeted molecules . In addition, their high quantum yield has gained importance as sensing probes over commonly used dyes, which is particularly helpful in single-particle detection required for biomedical assays. , …”

Section: Introductionmentioning

confidence: 99%

Imprinted Fluorescent Cellulose Membranes for the On-Site Detection of Myoglobin in Biological Media

Piloto

Ribeiro

Rodrigues

et al. 2021

ACS Appl. Bio Mater.

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In this work, the conjugation of molecularly imprinted polymers (MIPs) to quantum dots (QDs) was successfully applied in the assembly of an imprinted cellulose membrane [hydroxy ethyl cellulose (HEC)/MIP@QDs] for the specific recognition of the cardiac biomarker myoglobin (Myo) as a sensitive, user-friendly, and portable system with the potential for point-of-care (POC) applications. The concept is to use the MIPs as biorecognition elements, previously prepared on the surface of semiconductor cadmium telluride QDs as detection particles. The fluorescent quenching of the membrane occurred with increasing concentrations of Myo, showing linearity in the interval range of 7.39−291.3 pg/mL in a1000-fold diluted human serum. The best membrane showed a linear response below the cutoff values for myocardial infarction (23 ng/mL), a limit of detection of 3.08 pg/mL, and an imprinting factor of 1.65. The incorporation of the biorecognition element MIPs on the cellulose substrate brings an approach toward a portable and user-friendly device in a sustainable manner. Overall, the imprinted membranes display good stability and selectivity toward Myo when compared with the nonimprinted membranes (HEC/NIP@QDs) and have the potential to be applied as a sensitive system for Myo detection in the presence of other proteins. Moreover, the conjugation of MIPs to QDs increases the sensitivity of the system for an optical label-free detection method, reaching concentration levels with clinical significance.

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Stable, small, specific, low-valency quantum dots for single-molecule imaging

Cited by 24 publications

References 55 publications

Single quantum dot tracking illuminates neuroscience at the nanoscale

Single quantum dot tracking illuminates neuroscience at the nanoscale

A Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Dysregulation of the Neurovascular Unit Relevant to Brain Inflammation

Imprinted Fluorescent Cellulose Membranes for the On-Site Detection of Myoglobin in Biological Media

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