Stable transformation of Eustoma grandiflorum by particle bombardment

Takahashi, Misa; Nishihara, Masahiro; Yamamura, Saburo; Nishizawa, Shuji; Irifune, Kohei; Morikawa, Hiromichi

doi:10.1007/s002990050432

Cited by 15 publications

(7 citation statements)

References 17 publications

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“…The combination of BA with NAA is better for bud regeneration in lisianthus, as described previously by Takahashi et al 1998 andThiruvengadam andYang 2009. Crowded smaller shoots (\1 mm in height) derived from the explants were noticed at higher BA concentrations ([4.4 lM) (data not shown).…”

Section: Discussionmentioning

confidence: 54%

“…In the case of particle bombardment, 2 mg l -1 of bialaphos was used as a selective agent, because 3 mg l -1 of bialaphos appeared to inhibit lisianthus regeneration (Takahashi et al 1998). In this study, the non-transgenic leaf explants of lisianthus were sensitive to glufosinate ammonium.…”

Section: Discussionmentioning

confidence: 97%

“…It contains glufosinate and ensures excellent crop safety with a relatively low hazard to the operator. The bar gene was also used as a selection marker for the lisianthus genetic transformation by particle bombardment and the transgenic shoots were regenerated from the selection medium with 2 mg l -1 bialaphos (Takahashi et al 1998). The transgenic lisianthus plant was not evaluated of their herbicide-resistance in vitro or under greenhouse conditions.…”

Section: Discussionmentioning

confidence: 99%

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Transgenic Eustoma grandiflorum expressing the bar gene are resistant to the herbicide Basta®

Chen

Fang

Chiang

et al. 2010

Plant Cell Tiss Organ Cult

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Lisianthus (Eustoma grandiflorum) is a cut or ornamental flower that is popular all over the world. This ornamental crop, however, lacks an effective weed control method due to its susceptibility to herbicide. In this study, transgenic plants of a lisianthus cultivar were produced using Agrobacterium-mediated delivery of the plasmid pCAMBIA3300, which carried the bialaphos resistance (bar) gene under driven by the CaMV 35S promoter. The transgenic calli were derived from wounded edges of the leaves grown on a shoot regeneration medium containing 100 mg l -1 cefotaxime and 2 mg l -1 glufosinate ammonium for 4 weeks. The callus that was detached from the wounded edge of the leaf was transferred to the shoot regeneration medium with 100 mg l -1 cefotaxime and 5 mg l -1 glufosinate ammonium for 4 weeks for shoot regeneration. The bar gene integration and expression in the transgenic plants were confirmed by Southern and Northern blot analyses, respectively. Subsequently, the transgenic lines were assessed in vitro and under greenhouse conditions for their resistance to the commercial herbicide Basta Ò , which contains glufosinate ammonium as the active component. Six transgenic lines showed high percentages (67-80%) of survival in vitro under the selection condition with glufosinate ammonium (up to 216 mg l -1 ). Under greenhouse conditions, the plants from these six lines remained healthy and exhibited a normal phenotype after spraying with glufosinate ammonium (up to 1,350 mg l -1 ). This is the first paper to provide a detailed survey of transgenic lisianthus expressing the bar gene and exhibiting herbicide-resistance under greenhouse conditions.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Transgenic Eustoma grandiflorum expressing the bar gene are resistant to the herbicide Basta®

Chen

Fang

Chiang

et al. 2010

Plant Cell Tiss Organ Cult

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“…The particle bombardment of seedling explants of E. grandiflorum (Takahashi et al 1998) and cell suspension of G. trifora 9 G. scabra was successfully used (Hosokawa et al 2000). The electroporation of G. kurroo protoplasts derived from embryogenic cell suspension resulted in transformant regeneration via somatic embryogenesis (Wójcik and Rybczyński 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Polano-White and cv. Albireo (Takahashi et al 1998;Chen et al 2010;Semeria et al 1996;Hosokawa et al 2000;Nishihara et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation of gentian Gentiana tibetica (King) leaf explants with Agrobacterium tumefaciens strain C58C1

Wójcik

Rybczyński

2016

Acta Physiol Plant

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Experiments were carried out on transformation of leaf explants derived from the axenic culture of Gentiana tibetica (King) using a co-culture of Agrobacterium tumefaciens. A. tumefaciens octopine strain C58C1 carried neomycine phosphotransferase (nptII) and b-glucuronidase (uidA) genes. The influence of plasmid helper pCH32 on transformation efficiency was stressed. After co-cultivation, explant was cultured on three different regeneration media (RM1-3) at the presence of the timentin and callus formation and plants regeneration occurred. The transgenic character of the selected tissue and transformants T0 in the presence of kanamycin has been confirmed by the histochemical analysis of reporter enzyme activity (ß-glucuronidase), polymerase chain reaction, and southern hybridization detecting uidA and nptII genes. L-glutamine used during inoculation period had a significant influence on the transformation efficiency in relation to its concentration and time of treatment.

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Plant Transformation Technology: Particle Bombardment

Twyman

Christou

2004

Handbook of Plant Biotechnology

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Stable gene transfer to plants can be achieved by two general strategies— Agrobacterium ‐mediated transformation and the direct introduction of DNA into the plant cell. Agrobacterium ‐mediated transformation is dependent on complex biological interactions between the bacterium and host plant, while direct DNA transfer is based on chemical or physical principles. Techniques included in this category, however, require particular cell types as transformation targets, and it is not always possible to regenerate plants from such cells. Only particle bombardment, the introduction of DNA into plants using high‐velocity microprojectiles, has the potential to transform any cell type from any plant, providing a truly universal transformation strategy. In this chapter, we discuss the principles of particle bombardment and variables that must be optimised for efficient transient and stable transformation. We also discuss the fate of exogenous DNA once it has entered the plant cell, and how this influences transgene expression. A literature survey is presented, listing all the plant species that have been transformed using this method, up to the end of the year 2001.

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Stable transformation of Eustoma grandiflorum by particle bombardment

Cited by 15 publications

References 17 publications

Transgenic Eustoma grandiflorum expressing the bar gene are resistant to the herbicide Basta®

Transgenic Eustoma grandiflorum expressing the bar gene are resistant to the herbicide Basta®

Genetic transformation of gentian Gentiana tibetica (King) leaf explants with Agrobacterium tumefaciens strain C58C1

Plant Transformation Technology: Particle Bombardment

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