Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens

Rivero, Mercedes; Furman, Nicolás; Mencacci, Nicolás; Picca, Pablo; Toum, Laila; Lentz, Ezequiel Matías; Bravo-Almonacid, Fernando; Mentaberry, Alejandro

doi:10.1016/j.jbiotec.2011.11.005

Cited by 66 publications

(35 citation statements)

References 47 publications

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“…Indeed, peptides such as esculentin-1, dermaseptin SI and hCAP18/LL37 were used for the transformation of Nicotiana tabacum , Solanum tuberosum and Brassica rapa and conferred resistance to fungal and bacterial pathogens besides providing the plant with insecticidal properties [44], [45], [46]. Preliminary results indicate that G.max expressing the IAP gb|ABM17058.1|(213–231), a fragment of the enzyme D-myo-inositol 3-phosphate synthase, presented increased resistance to the Asian rust providing similar results to those obtained with natural antimicrobial peptides (Fig.…”

Section: Discussionmentioning

confidence: 99%

Probing Protein Sequences as Sources for Encrypted Antimicrobial Peptides

et al. 2012

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Starting from the premise that a wealth of potentially biologically active peptides may lurk within proteins, we describe here a methodology to identify putative antimicrobial peptides encrypted in protein sequences. Candidate peptides were identified using a new screening procedure based on physicochemical criteria to reveal matching peptides within protein databases. Fifteen such peptides, along with a range of natural antimicrobial peptides, were examined using DSC and CD to characterize their interaction with phospholipid membranes. Principal component analysis of DSC data shows that the investigated peptides group according to their effects on the main phase transition of phospholipid vesicles, and that these effects correlate both to antimicrobial activity and to the changes in peptide secondary structure. Consequently, we have been able to identify novel antimicrobial peptides from larger proteins not hitherto associated with such activity, mimicking endogenous and/or exogenous microorganism enzymatic processing of parent proteins to smaller bioactive molecules. A biotechnological application for this methodology is explored. Soybean (Glycine max) plants, transformed to include a putative antimicrobial protein fragment encoded in its own genome were tested for tolerance against Phakopsora pachyrhizi, the causative agent of the Asian soybean rust. This procedure may represent an inventive alternative to the transgenic technology, since the genetic material to be used belongs to the host organism and not to exogenous sources.

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Section: Discussionmentioning

confidence: 99%

Probing Protein Sequences as Sources for Encrypted Antimicrobial Peptides

et al. 2012

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“…The hemibiotrophic oomycete P. infestans causes potato blight and the fungus R. solani elicits brown canker on potato shoots and leaves (Osusky et al 2005;Rivero et al 2012). The hemibiotrophic oomycete P. infestans causes potato blight and the fungus R. solani elicits brown canker on potato shoots and leaves (Osusky et al 2005;Rivero et al 2012).…”

Section: Interaction Of S Tuberosum Varieties With Major Potato Pathmentioning

confidence: 99%

Profiling carbohydrate composition, biohydrogen capacity, and disease resistance in potato

Roldán¹,

Silva²,

Jeblick³

et al. 2013

Electron. J. Biotechnol.

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Background: Potato (Solanum tuberosum) is one of the most important sources of carbohydrates in human diet. Because of its high carbohydrate levels it recently has also received attention in biohydrogen production. To exploit the natural variation of potato with respect to resistance to major diseases, carbohydrate levels and composition, and capacity for biohydrogen production we analyzed tubers of native, improved, and genetically modified potatoes, and two other tuberous species for their glucose, fructose, sucrose, and starch content. Results: High-starch potato varieties were evaluated for their potential for Caldicellulosiruptor saccharolyticus-mediated biohydrogen production with Desirée and Rosita varieties delivering the highest biohydrogen amounts. Native line Vega1 and improved line Yagana were both immune to two isolates (A291, A287) of Phytophthora infestans. Conclusions: Our data demonstrate that native potato varieties might have great potential for further improving the multifaceted use of potato in worldwide food and biohydrogen production.

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“…The protein have been cloned from various plants such as Nicotiana tabaccum (Lui et al 1994, Evers et al 1999, Zuker et al 2001, Ouyang et al 2005, Qin et al 2006, Noori and Sokhansanj 2008, Parkhi et al 2009, Goel et al 2010, Husaini et al 2012, Subramanyam et al 2011, Solanum nigrum (Vasavirama and Kirti 2012), Capsicum annuum (Choi et al 2013), Solanum tuberosum (Rivero et al 2012), etc. The over-expression of osmotin have been reported to exhibit anti-fungal activity against diverse range of pathogens such as Puccinia triticina (Li et al 2015), Fusarium graminearum , Mackintosh et al 2007, Phytophthora capsici, Fusarium oxysporum (Zuker et al 2001, Mani et al 2012, Phytophthora infestans (Liu et al 1994, 1996, Li et al 1999, Rhizoctonia solani (Rivero et al 2012), etc. The anti-fungal property of the protein attributes to its capability to bind to domains present on the fungal plasma membrane (Thevissen et al 2005) which leads to dissipation of membrane integrity and can also inhibit spore germination through spore lysis (Koiwa et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of Osmotin (OsmSt) alleles from potato cultivar ‘Kufri Chipsona 1’

Kaur

Kumar

2020

Preprint

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The present study was focussed to clone and sequence characterise alleles of osmotin from cDNA of Solanum tuberosum L. cultivar 'Kufri Chipsona 1'. The genes vary in sizes as well as were found to have eleven point mutations throughout the coding sequence. One deletion of 7 bp was also found in smaller form of the gene having molecular weight of 19.91 KDa.Osmotin gene is known to impart resistance/tolerance to various fungal diseases in addition to its role as an osmoprotectant, thus, cloned osmotin alleles from important processing grade potato cultivar could become potential candidates for molecular breeding of potato.

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Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens

Cited by 66 publications

References 47 publications

Probing Protein Sequences as Sources for Encrypted Antimicrobial Peptides

Probing Protein Sequences as Sources for Encrypted Antimicrobial Peptides

Profiling carbohydrate composition, biohydrogen capacity, and disease resistance in potato

Cloning and characterization of Osmotin (OsmSt) alleles from potato cultivar ‘Kufri Chipsona 1’

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