Stage and Urinary Catecholamine Metabolite Excretion in Neuroblastoma

Pritchard, J. Frederick; Barnes, Janet M.; Germond, S.M.; Hartman, Oliver; Kraker, Jan de; Lewis, Ian C.; Lockwood, L.N.; Wallendszus, Karl

doi:10.1016/s0140-6736(89)92135-1

Cited by 21 publications

(5 citation statements)

References 5 publications

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“…To investigate the biochemical similarity of POB CM and POB UN series cell lines to human neuroblastoma tumors, we subsequently profiled patterns of catecholamine production within these tumors. The production of large quantities of catecholamines is a common feature of the majority of human neuroblastoma tumors, and an assay of catecholamine levels in the serum (norepinephrine, epinephrine, dopamine) and/or urine (homovanillic acid-HVA, vanillymandelic acid-VMA) of patients with neuroblastoma is used both for diagnostic purposes at the time of presentation and as a tumor marker that provides an indirect measure of disease burden during therapy (35,36). Looking at patterns of catecholamine production directly within the tumor microenvironment, we observed a marked overproduction of NE, EPI, and dopamine both within spontaneous tumors from MYCN transgenic mice and the respective POB CM and POB UN series tumors.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Molecular and Histopathologic Profiling of Tumor Tissue in a Novel Transplantable Model of Murine Neuroblastoma: New Tools for Pediatric Drug Discovery

Stauffer

Orentas

Lincoln

et al. 2012

Cancer Investigation

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Using two MYCN transgenic mouse strains, we established 10 transplantable neuroblastoma cell lines via serial orthotopic passage in the adrenal gland. Tissue arrays demonstrate that by histochemistry, vascularity, immunohistochemical staining for neuroblastoma markers, catecholamine analysis, and concurrent cDNA microarray analysis, there is a close correspondence between the transplantable lines and the spontaneous tumors. Several genes closely associated with the pathobiology and immune evasion of neuroblastoma, novel targets that warrant evaluation, and decreased expression of tumor suppressor genes are demonstrated. These studies describe a unique and generalizable approach to expand the utility of transgenic models of spontaneous tumor, providing new tools for preclinical investigation.

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Section: Discussionmentioning

confidence: 99%

High-Throughput Molecular and Histopathologic Profiling of Tumor Tissue in a Novel Transplantable Model of Murine Neuroblastoma: New Tools for Pediatric Drug Discovery

Stauffer

Orentas

Lincoln

et al. 2012

Cancer Investigation

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“…As a result, the timely and effective diagnosis of NB is very significant. Urinary homovanillic acid (HVA) and vanillylmandelic acid (VMA), which are terminal metabolites of catecholamines, serve as biomarkers to support clinical diagnosis of NB (Brodeur et al, ; Pritchard et al, ). Traditionally, the measurement of HVA and VMA was performed on high‐performance liquid chromatography coupled with electrochemical detection (HPLC‐ECD) or fluorescence detection (FLD) (Davidson, ; Manickum, ; Mercolini, Gerra, Consorti, Somanini, & Raggi, ; Barco et al, ), and gas chromatography–mass spectrometry (GC–MS) (Cole et al, ; Fauler et al, ; Park, Hong, Shin, & Hong, ; Tran et al, ).…”

Section: Introductionmentioning

confidence: 99%

Analytical validation and clinical application of urinary vanillylmandelic acid and homovanillic acid by LC–MS/MS for diagnosis of neuroblastoma

Shen

Lü

et al. 2019

Biomedical Chromatography

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Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are clinical biomarkers for diagnosis of neuroblastoma (NB), which commonly occurs in the childhood. Development and application of a robust LC-MS/MS method for fast determination of these biomarkers for optimal laboratory testing of NB is essential in clinical laboratories. In present study, we developed and validated a simple liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quick clinical testing of VMA and HVA for diagnosis of NB. The method was validated according to the current CLSI C62-A and FDA guidelines. The age-adjusted pediatric reference intervals and diagnostic performance were evaluated in both 24 h urine and random urine. Injection-toinjection time was 3.5 min. Inter-and intra-assay coefficients of variation (CVs) were ≤3.88%. The lower limit of quantification and the limit of detection were 0.50 and 0.25 μmol/L for both VMA and HVA. Recoveries of VMA and HVA were in the ranges of 85-109% and 86-100% with CVs ≤5.76%. This method was free from significant matrix effect, carryover and interference. The establishment of age-adjusted pediatric reference intervals by this LC-MS/MS method was favorable for the improvement in diagnostic performance, which was crucial for correct interpretation of test results from children in both 24 h and random urine.

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“…Patients diagnosed with NB usually have elevated hematic and urinary concentration of catecholamines or their metabolites. Both urinary homovanillic and vanillylmandelic acid (HVA and VMA) are catecholamine metabolites that have been used as biomarkers in the diagnosis and follow-up of patients with NB [ 49 , 50 ]. Elevated levels of these metabolites suggest, therefore, that the adrenergic system could play an important role in regulating NB biology; indeed, recent findings have demonstrated that β -ARs modulation, thorough the use of β -blockers, affects NB tumor growth and progression.…”

Section: Beta-adrenergic Receptors In Nbmentioning

confidence: 99%

Beta-Blockers and Berberine: A Possible Dual Approach to Contrast Neuroblastoma Growth and Progression

Calvani

Subbiani

Bruno

et al. 2020

Oxidative Medicine and Cellular Longevity

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The use of nutraceuticals during cancer treatment is a long-lasting debate. Berberine (BBR) is an isoquinoline quaternary alkaloid extracted from a variety of medicinal plants. BBR has been shown to have therapeutic effects in different pathologies, particularly in cancer, where it affects pathways involved in tumor progression. In neuroblastoma, the most common extracranial childhood solid tumor, BBR, reduces tumor growth by regulating both stemness and differentiation features and by inducing apoptosis. At the same time, the inhibition of β-adrenergic signaling leads to a reduction in growth and increase of differentiation of neuroblastoma. In this review, we summarize the possible beneficial effects of BBR in counteracting tumor growth and progression in various types of cancer and, in particular, in neuroblastoma. However, BBR administration, besides its numerous beneficial effects, presents a few side effects due to inhibition of MAO A enzyme in neuroblastoma cells. Therefore, herein, we proposed a novel therapeutic strategy to overcome side effects of BBR administration consisting of concomitant administration of BBR together with β-blockers in neuroblastoma.

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Stage and Urinary Catecholamine Metabolite Excretion in Neuroblastoma

Cited by 21 publications

References 5 publications

High-Throughput Molecular and Histopathologic Profiling of Tumor Tissue in a Novel Transplantable Model of Murine Neuroblastoma: New Tools for Pediatric Drug Discovery

High-Throughput Molecular and Histopathologic Profiling of Tumor Tissue in a Novel Transplantable Model of Murine Neuroblastoma: New Tools for Pediatric Drug Discovery

Analytical validation and clinical application of urinary vanillylmandelic acid and homovanillic acid by LC–MS/MS for diagnosis of neuroblastoma

Beta-Blockers and Berberine: A Possible Dual Approach to Contrast Neuroblastoma Growth and Progression

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