Stage D1 Prostatic Adenocarcinoma: Significance of Nuclear DNA Ploidy Patterns Studied by Flow Cytometry

Winkler, Harry; Rainwater, Leslie M.; Myers, Robert P.; Farrow, George M.; Therneau, Terry M.; Zincke, Horst; Lieber, Michael M.

doi:10.1016/s0025-6196(12)64942-8

Mayo Clinic Proceedings

1988

DOI: 10.1016/s0025-6196(12)64942-8

|View full text |Cite

Stage D1 Prostatic Adenocarcinoma: Significance of Nuclear DNA Ploidy Patterns Studied by Flow Cytometry

Harry Winkler

Leslie M. Rainwater

Robert P. Myers

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1990

2001

Publication Types

Select...

Article9

Relationship

Self Cite0

Independent9

Authors

Journals

Cited by 161 publications

(42 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the majority of the investigated PC specimens (73%) demonstrate a surprising lack of both structural and numerical chromosomal changes, resulting in essentially normal diploid karyotypes (3,8,141. In contrast, using DNA flow cytometry (FCM) > 50% of the PCs were shown to have one or more DNA aneuploid cell populations (unpublished results; 11,15,16,22). The causes for this observed discrepancy between cytogenetic and FCM results have not been identified until now.…”

mentioning

confidence: 99%

Preferential loss of abnormal prostate carcinoma cells by collagenase treatment

König¹,

Dongen²,

Schröder³

1993

Cytometry

View full text Add to dashboard Cite

Effects of two different methods of tumor disaggregation on flow cytometric ploidy distribution and intact cell yield were investigated. Either mechanical disaggregation or collagenase digestion was applied to 35 prostate tumor specimens. Seven collagenase-treated samples failed to yield any intact cells, whereas with mechanical disaggregation in all cases a sufficient number of intact cells were obtained. No differences in the FCM ploidy distribution of tumors with a DNA diploid stemline were observed comparing both techniques. In DNA aneuploid tumors, however, collagenase treatment had an adverse effect on the abnormal cell populations. In 14/17 of such tumors, the abnormal cell populations were significantly reduced; in eight of these the percentage of DNA aneuploid cells declined even below the minimum percentage (10%) that was defined for DNA aneuploidy. Since collagenase is a widely used enzyme for tissue disaggregation, especially in tumor cytogenetics, the presented data will have consequences for the interpretation of results obtained by methods involving the use of this enzyme.

show abstract

mentioning

confidence: 99%

Preferential loss of abnormal prostate carcinoma cells by collagenase treatment

König¹,

Dongen²,

Schröder³

1993

Cytometry

View full text Add to dashboard Cite

show abstract

“…DNA ploidy (1)(2)(3)(4)(5)(6) and proliferation (7,8) have been shown to provide prognostic information for prostate cancer. Studies examining the relationship between ploidy and proliferation and patient outcome are often based upon flow cytometry (FCM) data using formalin-fixed, paraffin-embedded tissue from radical retropubic prostatectomy (RRP) specimens.…”

mentioning

confidence: 99%

Factors That Influence the Measurement of Prostate Cancer DNA Ploidy and Proliferation in Paraffin Embedded Tissue Evaluated by Flow Cytometry

Cheville

Katzmann

et al. 2001

Modern Pathology

View full text Add to dashboard Cite

DNA ploidy (1-6) and proliferation (7,8) have been shown to provide prognostic information for prostate cancer. Studies examining the relationship between ploidy and proliferation and patient outcome are often based upon flow cytometry (FCM) data using formalin-fixed, paraffin-embedded tissue from radical retropubic prostatectomy (RRP) specimens. However, FCM cannot readily distinguish among benign epithelial, stromal, and inflammatory cells, high grade prostatic intraepithelial neoplasia (HGPIN), and cancer cells. Furthermore, studies that do not show DNA ploidy and proliferation as independent prognostic markers emphasize the strong correlation of cancer Gleason score with DNA ploidy and proliferation (9 -11).To the best of our knowledge, there are no published reports directly assessing the degree to which non-invasive tumor components in paraffinembedded tissue blocks are associated with prostate cancer ploidy and proliferation results determined by FCM. In this study, we evaluated hematoxylin and eosin (H&E) histologic features from 322 RRP formalin-fixed, paraffin-embedded tissue specimens used for determining DNA ploidy,

show abstract

“…Several studies indicate that prostate cancer patients with DNA-aneuploid tumours have shorter disease-free and overall survival than those with DNA-diploid tumours (Fordham et al, 1986;Lee et al, 1988;Montgomery et al, 1990;Nativ et al, 1989;Stephenson et al, 1987;Winkler et al, 1988). However, it is still controversial whether aneuploidy is an independent prognostic indicator.…”

mentioning

confidence: 99%

Flow cytometric analysis of DNA ploidy and S-phase fraction from prostatic carcinomas: implications for prognosis and response to endocrine therapy

Visakorpi

Kallioniemi²,

Paronen³

et al. 1991

Br J Cancer

View full text Add to dashboard Cite

Summary We analysed ploidy and S-phase fraction (SPF) from 78 paraffin-embedded primary prostatic carcinomas by DNA flow cytometry. DNA aneuploidy and above median (4.2%) SPF were both associated with high tumour grade, large size of prostate and presence of distant metastases. Both aneuploidy and high SPF (>4.2%) indicated short 10-year progression-free (P = 0.01 for ploidy and P = 0.0002 for SPF), overall (P = 0.004 and P <0.0001) as well as prostate cancer survival (P = 0.002 and P <0.0001). Ten-year overall survival rate was 45% in cases with SPF below 4.2% and 0% in those with higher values, whereas the corresponding prostate cancer-specific survival rates were 80% and 11%, respectively. None of the seven tumours with SPF above 12% showed an objective response to endocrine therapy, whereas 26/49 (52%) of those with lower SPF values responded (P = 0.01). DNA ploidy, tumour grade, T-stage or M-stage did not significantly correlate with endocrine responsiveness. SPF was also the best predictor of progression free survival in patients treated hormonally. In conclusion, dletection of high SPF in prostate cancer may indicate lack of hormonal responsiveness and poor prognosis.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Stage D1 Prostatic Adenocarcinoma: Significance of Nuclear DNA Ploidy Patterns Studied by Flow Cytometry

Cited by 161 publications

References 24 publications

Preferential loss of abnormal prostate carcinoma cells by collagenase treatment

Preferential loss of abnormal prostate carcinoma cells by collagenase treatment

Factors That Influence the Measurement of Prostate Cancer DNA Ploidy and Proliferation in Paraffin Embedded Tissue Evaluated by Flow Cytometry

Flow cytometric analysis of DNA ploidy and S-phase fraction from prostatic carcinomas: implications for prognosis and response to endocrine therapy

Contact Info

Product

Resources

About